Estudo comparativo de diferentes técnicas de avaliação da concentração espermática em suínos

• Autor(a) principal: Murgas, L. D. S.
• Data de Publicação: 2010
• Outros Autores: Lima, D., Alvarenga, A. L. N., Zangeronimo, M. G., Oberlender, G., Oliveira, S. L.
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UFLA
• Texto Completo: http://repositorio.ufla.br/jspui/handle/1/39167
• topic: Espectrofotômetro; Espermiodensímetro; Câmara de Neubauer; Spectrofotometer; Spermdensimeter; Neubauer chamber

The precise and accurate determination of the spermatic concentration of the ejaculate is important in the production of insemination doses with a correct number of spermatozoa. The objective of this work was to compare the efficiency of some measurement techniques for spermatic concentration in the semen and to determine prediction equations, establishing a correlation among them. It were collected 93 ejaculations, its spermatic concentration was determined by direct (Neubauer chamber, NC) or indirect (spermdensimeter, SD and spectro-photometer, SP) methods. A randomized block design was utilized, with 93 repetitions and two analysis per experimental unit. Data were submitted to variance analysis, the SNK test was applied to means and the treatments were interrelated by means od adjustment of linear models among results (prediction equation). It was not difference in the determination of the spermatic concentration obtained with SD (361.7 x 106 sptz/ml) and SP (343.7 x 106 sptz/ml), but these values were higher than the NC (245.3 x 106 sptz/ml). The equations obtained were SD= -0.30 [ejaculate volume (in ml)] + 0.61 SP + 213.47 (R2= 0.72) and SD= -0.50 [ejaculate volume (in ml)] + 0.47 NC (R2= 0.7). The SD and SP measurement techniques of the spermatic concentration overestimate values with regard to CN. New research is need to determine the true efficiency of these techniques in farms.