Comparison of three laboratorial tests for diagnosis of canine parvovirus infection

Bibliographic Details
Main Author: Silva,M.M.O.
Publication Date: 2013
Other Authors: Castro,T.X., Costa,E.M., Trancoso,T.A.L., Mendes-de-Almeida,F., Labarthe,N.V., Cubel Garcia,R.C.N.
Format: Article
Language: eng
Source: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100023
Summary: The aim of this study was to evaluate the rapid tests currently used for canine parvovirus (CPV) diagnosis: hemagglutination test (HA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). A total of 112 fecal samples collected from diarrheic puppies up to one year of age were tested. The EIA was able to detect CPV antigen in 44 samples. By HA, 32 samples tested highly positive with titers &gt;128, eight tested weakly positive (titers 32 and 64) and 72 were negative (titers <16). Using PCR, 57 samples were found positive including 13 EIA-negative and 19 HA-negative samples. The best correlation was observed between EIA and PCR (88.4%). These tests were able to detect all types of CPV, including CPV-2c. Considering that 23%-33% of dogs presenting enteritis did not show infection by EIA nor HA, negative results from the antigen detection tests should be confirmed through molecular methods.
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spelling Comparison of three laboratorial tests for diagnosis of canine parvovirus infectioncanine parvovirusenteritisHAEIAPCRThe aim of this study was to evaluate the rapid tests currently used for canine parvovirus (CPV) diagnosis: hemagglutination test (HA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). A total of 112 fecal samples collected from diarrheic puppies up to one year of age were tested. The EIA was able to detect CPV antigen in 44 samples. By HA, 32 samples tested highly positive with titers &gt;128, eight tested weakly positive (titers 32 and 64) and 72 were negative (titers <16). Using PCR, 57 samples were found positive including 13 EIA-negative and 19 HA-negative samples. The best correlation was observed between EIA and PCR (88.4%). These tests were able to detect all types of CPV, including CPV-2c. Considering that 23%-33% of dogs presenting enteritis did not show infection by EIA nor HA, negative results from the antigen detection tests should be confirmed through molecular methods.Universidade Federal de Minas Gerais, Escola de Veterinária2013-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100023Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.65 n.1 2013reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352013000100023info:eu-repo/semantics/openAccessSilva,M.M.O.Castro,T.X.Costa,E.M.Trancoso,T.A.L.Mendes-de-Almeida,F.Labarthe,N.V.Cubel Garcia,R.C.N.eng2013-03-05T00:00:00Zoai:scielo:S0102-09352013000100023Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2013-03-05T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Comparison of three laboratorial tests for diagnosis of canine parvovirus infection
title Comparison of three laboratorial tests for diagnosis of canine parvovirus infection
spellingShingle Comparison of three laboratorial tests for diagnosis of canine parvovirus infection
Silva,M.M.O.
canine parvovirus
enteritis
HA
EIA
PCR
title_short Comparison of three laboratorial tests for diagnosis of canine parvovirus infection
title_full Comparison of three laboratorial tests for diagnosis of canine parvovirus infection
title_fullStr Comparison of three laboratorial tests for diagnosis of canine parvovirus infection
title_full_unstemmed Comparison of three laboratorial tests for diagnosis of canine parvovirus infection
title_sort Comparison of three laboratorial tests for diagnosis of canine parvovirus infection
author Silva,M.M.O.
author_facet Silva,M.M.O.
Castro,T.X.
Costa,E.M.
Trancoso,T.A.L.
Mendes-de-Almeida,F.
Labarthe,N.V.
Cubel Garcia,R.C.N.
author_role author
author2 Castro,T.X.
Costa,E.M.
Trancoso,T.A.L.
Mendes-de-Almeida,F.
Labarthe,N.V.
Cubel Garcia,R.C.N.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,M.M.O.
Castro,T.X.
Costa,E.M.
Trancoso,T.A.L.
Mendes-de-Almeida,F.
Labarthe,N.V.
Cubel Garcia,R.C.N.
dc.subject.por.fl_str_mv canine parvovirus
enteritis
HA
EIA
PCR
topic canine parvovirus
enteritis
HA
EIA
PCR
description The aim of this study was to evaluate the rapid tests currently used for canine parvovirus (CPV) diagnosis: hemagglutination test (HA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). A total of 112 fecal samples collected from diarrheic puppies up to one year of age were tested. The EIA was able to detect CPV antigen in 44 samples. By HA, 32 samples tested highly positive with titers &gt;128, eight tested weakly positive (titers 32 and 64) and 72 were negative (titers <16). Using PCR, 57 samples were found positive including 13 EIA-negative and 19 HA-negative samples. The best correlation was observed between EIA and PCR (88.4%). These tests were able to detect all types of CPV, including CPV-2c. Considering that 23%-33% of dogs presenting enteritis did not show infection by EIA nor HA, negative results from the antigen detection tests should be confirmed through molecular methods.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100023
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-09352013000100023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.65 n.1 2013
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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