Comparison of three laboratorial tests for diagnosis of canine parvovirus infection
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Publication Date: | 2013 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng |
Source: | Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100023 |
Summary: | The aim of this study was to evaluate the rapid tests currently used for canine parvovirus (CPV) diagnosis: hemagglutination test (HA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). A total of 112 fecal samples collected from diarrheic puppies up to one year of age were tested. The EIA was able to detect CPV antigen in 44 samples. By HA, 32 samples tested highly positive with titers >128, eight tested weakly positive (titers 32 and 64) and 72 were negative (titers <16). Using PCR, 57 samples were found positive including 13 EIA-negative and 19 HA-negative samples. The best correlation was observed between EIA and PCR (88.4%). These tests were able to detect all types of CPV, including CPV-2c. Considering that 23%-33% of dogs presenting enteritis did not show infection by EIA nor HA, negative results from the antigen detection tests should be confirmed through molecular methods. |
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Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
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Comparison of three laboratorial tests for diagnosis of canine parvovirus infectioncanine parvovirusenteritisHAEIAPCRThe aim of this study was to evaluate the rapid tests currently used for canine parvovirus (CPV) diagnosis: hemagglutination test (HA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). A total of 112 fecal samples collected from diarrheic puppies up to one year of age were tested. The EIA was able to detect CPV antigen in 44 samples. By HA, 32 samples tested highly positive with titers >128, eight tested weakly positive (titers 32 and 64) and 72 were negative (titers <16). Using PCR, 57 samples were found positive including 13 EIA-negative and 19 HA-negative samples. The best correlation was observed between EIA and PCR (88.4%). These tests were able to detect all types of CPV, including CPV-2c. Considering that 23%-33% of dogs presenting enteritis did not show infection by EIA nor HA, negative results from the antigen detection tests should be confirmed through molecular methods.Universidade Federal de Minas Gerais, Escola de Veterinária2013-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100023Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.65 n.1 2013reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352013000100023info:eu-repo/semantics/openAccessSilva,M.M.O.Castro,T.X.Costa,E.M.Trancoso,T.A.L.Mendes-de-Almeida,F.Labarthe,N.V.Cubel Garcia,R.C.N.eng2013-03-05T00:00:00Zoai:scielo:S0102-09352013000100023Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2013-03-05T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Comparison of three laboratorial tests for diagnosis of canine parvovirus infection |
title |
Comparison of three laboratorial tests for diagnosis of canine parvovirus infection |
spellingShingle |
Comparison of three laboratorial tests for diagnosis of canine parvovirus infection Silva,M.M.O. canine parvovirus enteritis HA EIA PCR |
title_short |
Comparison of three laboratorial tests for diagnosis of canine parvovirus infection |
title_full |
Comparison of three laboratorial tests for diagnosis of canine parvovirus infection |
title_fullStr |
Comparison of three laboratorial tests for diagnosis of canine parvovirus infection |
title_full_unstemmed |
Comparison of three laboratorial tests for diagnosis of canine parvovirus infection |
title_sort |
Comparison of three laboratorial tests for diagnosis of canine parvovirus infection |
author |
Silva,M.M.O. |
author_facet |
Silva,M.M.O. Castro,T.X. Costa,E.M. Trancoso,T.A.L. Mendes-de-Almeida,F. Labarthe,N.V. Cubel Garcia,R.C.N. |
author_role |
author |
author2 |
Castro,T.X. Costa,E.M. Trancoso,T.A.L. Mendes-de-Almeida,F. Labarthe,N.V. Cubel Garcia,R.C.N. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Silva,M.M.O. Castro,T.X. Costa,E.M. Trancoso,T.A.L. Mendes-de-Almeida,F. Labarthe,N.V. Cubel Garcia,R.C.N. |
dc.subject.por.fl_str_mv |
canine parvovirus enteritis HA EIA PCR |
topic |
canine parvovirus enteritis HA EIA PCR |
description |
The aim of this study was to evaluate the rapid tests currently used for canine parvovirus (CPV) diagnosis: hemagglutination test (HA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). A total of 112 fecal samples collected from diarrheic puppies up to one year of age were tested. The EIA was able to detect CPV antigen in 44 samples. By HA, 32 samples tested highly positive with titers >128, eight tested weakly positive (titers 32 and 64) and 72 were negative (titers <16). Using PCR, 57 samples were found positive including 13 EIA-negative and 19 HA-negative samples. The best correlation was observed between EIA and PCR (88.4%). These tests were able to detect all types of CPV, including CPV-2c. Considering that 23%-33% of dogs presenting enteritis did not show infection by EIA nor HA, negative results from the antigen detection tests should be confirmed through molecular methods. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100023 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-09352013000100023 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.65 n.1 2013 reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online) instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
collection |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository.name.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br |
_version_ |
1750220886271590400 |