Addition of chlorogenic acid and caffeine during the processing of cooled boar semen
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200489 |
Resumo: | ABSTRACT A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P <0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P <0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P<0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P <0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P <0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition. |
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Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
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Addition of chlorogenic acid and caffeine during the processing of cooled boar semenantioxidantboar semen storagemetabolic activatormethylxanthinepolyphenolABSTRACT A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P <0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P <0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P<0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P <0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P <0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.Universidade Federal de Minas Gerais, Escola de Veterinária2019-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200489Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.71 n.2 2019reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/1678-4162-10415info:eu-repo/semantics/openAccessPereira,B.A.Rocha,L.G.P.Teles,M.C.Silva,W.E.Barbosa,J.A.Rabelo,S.S.Uchoa,A.S.Rodrıguez-Gil,J. E.Pereira,L.J.Zangeronimo,M.G.eng2019-06-03T00:00:00Zoai:scielo:S0102-09352019000200489Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2019-06-03T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Addition of chlorogenic acid and caffeine during the processing of cooled boar semen |
title |
Addition of chlorogenic acid and caffeine during the processing of cooled boar semen |
spellingShingle |
Addition of chlorogenic acid and caffeine during the processing of cooled boar semen Pereira,B.A. antioxidant boar semen storage metabolic activator methylxanthine polyphenol |
title_short |
Addition of chlorogenic acid and caffeine during the processing of cooled boar semen |
title_full |
Addition of chlorogenic acid and caffeine during the processing of cooled boar semen |
title_fullStr |
Addition of chlorogenic acid and caffeine during the processing of cooled boar semen |
title_full_unstemmed |
Addition of chlorogenic acid and caffeine during the processing of cooled boar semen |
title_sort |
Addition of chlorogenic acid and caffeine during the processing of cooled boar semen |
author |
Pereira,B.A. |
author_facet |
Pereira,B.A. Rocha,L.G.P. Teles,M.C. Silva,W.E. Barbosa,J.A. Rabelo,S.S. Uchoa,A.S. Rodrıguez-Gil,J. E. Pereira,L.J. Zangeronimo,M.G. |
author_role |
author |
author2 |
Rocha,L.G.P. Teles,M.C. Silva,W.E. Barbosa,J.A. Rabelo,S.S. Uchoa,A.S. Rodrıguez-Gil,J. E. Pereira,L.J. Zangeronimo,M.G. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pereira,B.A. Rocha,L.G.P. Teles,M.C. Silva,W.E. Barbosa,J.A. Rabelo,S.S. Uchoa,A.S. Rodrıguez-Gil,J. E. Pereira,L.J. Zangeronimo,M.G. |
dc.subject.por.fl_str_mv |
antioxidant boar semen storage metabolic activator methylxanthine polyphenol |
topic |
antioxidant boar semen storage metabolic activator methylxanthine polyphenol |
description |
ABSTRACT A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P <0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P <0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P<0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P <0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P <0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200489 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200489 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4162-10415 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.71 n.2 2019 reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online) instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
collection |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository.name.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br |
_version_ |
1750220892823093248 |