Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.1016/j.msec.2016.09.009 http://hdl.handle.net/1843/40170 |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2022-03-17T12:50:58Z2022-03-17T12:50:58Z2017-0170535543https://doi.org/10.1016/j.msec.2016.09.00909284931http://hdl.handle.net/1843/40170CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOutra AgênciaBovine serum albumin (BSA) has been recognized as a marker of the cow's health, milk quality, an allergenic protein and as a carrier. Its detection is important in the food, pharmaceutical and medical industries. However, traditional techniques used to detect BSA are often time-consuming, expensive, and show limited sensitivity. This paper describes properties of polydiacetylene-triblock copolymer (L64) nanosensors, synthesized to easily detect BSA. Sensor efficiency was studied as a function of nanosensor composition, polydiacetylene chemical structures, BSA conformation and hydrophobic domain availability, using spectroscopic, calorimetric, light scattering, and electrokinetic analyses. Nanosensors were sensitive to detect the average BSA concentration of milk and dairy products and discriminated between native and denatured protein through naked-eye detectable blue-to-red transition. The standard Gibbs free energy (− 10.44 < ΔG° < − 49.52 kJ M), stoichiometry complex (1 < “n” < 3), and binding constant (6.7 × 102 < Ka < 4.79 × 108 M− 1) of BSA-nanosensor complex formation established a direct relationship between nanosensor response and BSA-nanosensor interaction. BSA-nanosensor interaction was entropically (without cholesterol), and enthalpically driven (with cholesterol). Eugenol-BSA complex did not induce colorimetric transition. Polydiacetylene-L64 nanosensors are potential low-cost sensors for rapid detection of BSA, discriminating between native/denatured and free/bound protein.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASMaterials Science and Engineering: CAlbuminaCopolímerosLeite - ProteínasCalorimetriaPolydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.sciencedirect.com/science/article/pii/S0928493116311821?via%3Dihub#!Jaqueline de Paula RezendeGuilherme Max Dias FerreiraGabriel Max Dias FerreiraLuis Henrique Mendes da SilvaMaria do Carmo Hespanhol da SilvaMaximiliano Soares PintoAna Clarissa dos Santos Piresinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGORIGINALPolydiacetylenetriblock copolymer nanosensor for the detection of native and free bovine serum albumin.pdfPolydiacetylenetriblock copolymer nanosensor for the detection of native and free bovine serum albumin.pdfapplication/pdf653339https://repositorio.ufmg.br/bitstream/1843/40170/2/Polydiacetylenetriblock%20copolymer%20nanosensor%20for%20the%20detection%20of%20native%20and%20free%20bovine%20serum%20albumin.pdfc5fbd7e1c6c7209ad4be5b5a16b62556MD52LICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/40170/1/License.txtfa505098d172de0bc8864fc1287ffe22MD511843/401702022-03-17 09:50:58.424oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-03-17T12:50:58Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin |
| title |
Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin |
| spellingShingle |
Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin Jaqueline de Paula Rezende Albumina Copolímeros Leite - Proteínas Calorimetria |
| title_short |
Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin |
| title_full |
Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin |
| title_fullStr |
Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin |
| title_full_unstemmed |
Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin |
| title_sort |
Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin |
| author |
Jaqueline de Paula Rezende |
| author_facet |
Jaqueline de Paula Rezende Guilherme Max Dias Ferreira Gabriel Max Dias Ferreira Luis Henrique Mendes da Silva Maria do Carmo Hespanhol da Silva Maximiliano Soares Pinto Ana Clarissa dos Santos Pires |
| author_role |
author |
| author2 |
Guilherme Max Dias Ferreira Gabriel Max Dias Ferreira Luis Henrique Mendes da Silva Maria do Carmo Hespanhol da Silva Maximiliano Soares Pinto Ana Clarissa dos Santos Pires |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Jaqueline de Paula Rezende Guilherme Max Dias Ferreira Gabriel Max Dias Ferreira Luis Henrique Mendes da Silva Maria do Carmo Hespanhol da Silva Maximiliano Soares Pinto Ana Clarissa dos Santos Pires |
| dc.subject.other.pt_BR.fl_str_mv |
Albumina Copolímeros Leite - Proteínas Calorimetria |
| topic |
Albumina Copolímeros Leite - Proteínas Calorimetria |
| description |
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| publishDate |
2017 |
| dc.date.issued.fl_str_mv |
2017-01 |
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2022-03-17T12:50:58Z |
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2022-03-17T12:50:58Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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http://hdl.handle.net/1843/40170 |
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https://doi.org/10.1016/j.msec.2016.09.009 |
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09284931 |
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https://doi.org/10.1016/j.msec.2016.09.009 http://hdl.handle.net/1843/40170 |
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eng |
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Materials Science and Engineering: C |
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openAccess |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
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Universidade Federal de Minas Gerais |
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