Characterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functions
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.3389/fmicb.2019.02008 http://hdl.handle.net/1843/56331 https://orcid.org/0000-0003-3783-5717 https://orcid.org/0000-0002-6944-3008 https://orcid.org/0000-0003-4720-6746 https://orcid.org/0000-0002-4255-3047 https://orcid.org/0000-0001-9152-8756 https://orcid.org/0000-0001-8186-8714 https://orcid.org/0000-0001-9799-773X https://orcid.org/0000-0002-1108-5666 |
Resumo: | Extracellular vesicles (EVs) has been considered an alternative process for intercellular communication. EVs release by filamentous fungi and the role of vesicular secretion during fungus-host cells interaction remain unknown. Here, we identified the secretion of EVs from the pathogenic filamentous fungus, Aspergillus fumigatus. Analysis of the structure of EVs demonstrated that A. fumigatus produces round shaped bilayer structures ranging from 100 to 200 nm size, containing ergosterol and a myriad of proteins involved in REDOX, cell wall remodeling and metabolic functions of the fungus. We demonstrated that macrophages can phagocytose A. fumigatus EVs. Phagocytic cells, stimulated with EVs, increased fungal clearance after A. fumigatus conidia challenge. EVs were also able to induce the production of TNF-α and CCL2 by macrophages and a synergistic effect was observed in the production of these mediators when the cells were challenged with the conidia. In bone marrow-derived neutrophils (BMDN) treated with EVs, there was enhancement of the production of TNF-α and IL-1β in response to conidia. Together, our results demonstrate, for the first time, that A. fumigatus produces EVs containing a diverse set of proteins involved in fungal physiology and virulence. Moreover, EVs are biologically active and stimulate production of inflammatory mediators and fungal clearance. |
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2023-07-14T22:45:34Z2023-07-14T22:45:34Z201910113https://doi.org/10.3389/fmicb.2019.020081664-302Xhttp://hdl.handle.net/1843/56331https://orcid.org/0000-0003-3783-5717https://orcid.org/0000-0002-6944-3008https://orcid.org/0000-0003-4720-6746https://orcid.org/0000-0002-4255-3047https://orcid.org/0000-0001-9152-8756https://orcid.org/0000-0001-8186-8714https://orcid.org/0000-0001-9799-773Xhttps://orcid.org/0000-0002-1108-5666Extracellular vesicles (EVs) has been considered an alternative process for intercellular communication. EVs release by filamentous fungi and the role of vesicular secretion during fungus-host cells interaction remain unknown. Here, we identified the secretion of EVs from the pathogenic filamentous fungus, Aspergillus fumigatus. Analysis of the structure of EVs demonstrated that A. fumigatus produces round shaped bilayer structures ranging from 100 to 200 nm size, containing ergosterol and a myriad of proteins involved in REDOX, cell wall remodeling and metabolic functions of the fungus. We demonstrated that macrophages can phagocytose A. fumigatus EVs. Phagocytic cells, stimulated with EVs, increased fungal clearance after A. fumigatus conidia challenge. EVs were also able to induce the production of TNF-α and CCL2 by macrophages and a synergistic effect was observed in the production of these mediators when the cells were challenged with the conidia. In bone marrow-derived neutrophils (BMDN) treated with EVs, there was enhancement of the production of TNF-α and IL-1β in response to conidia. Together, our results demonstrate, for the first time, that A. fumigatus produces EVs containing a diverse set of proteins involved in fungal physiology and virulence. Moreover, EVs are biologically active and stimulate production of inflammatory mediators and fungal clearance.porUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIAICB - DEPARTAMENTO DE FARMACOLOGIAICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICAICB - DEPARTAMENTO DE MICROBIOLOGIAFrontiers in MicrobiologyAspergillus fumigatusMacrófagosNeutrofilosExtracellular vesiclesFlamentous fungusAspergillus fumigatusHost-pathogen interactionsMacrophagesNeutrophilsCharacterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.frontiersin.org/articles/10.3389/fmicb.2019.02008/fullJéssica Amanda Marques SouzaFrédéric Jean Georges FrézardDaniele da Glória de SouzaMauro Martins TeixeiraFrederico Marianetti SorianiLudmila de Matos BaltazarVirgínia Mendes CarregalLudmila Gouveia EufrasioAndré Gustavo de OliveiraWendell Girard DiasKildare Rocha de MirandaIran MalavaziDaniel de Assis Santosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/56331/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALCharacterization of Aspergillus fumigatus Extracellular Vesicles and Their Effects on Macrophages and Neutrophils Functions.pdfCharacterization of Aspergillus fumigatus Extracellular Vesicles and Their Effects on Macrophages and Neutrophils Functions.pdfapplication/pdf2118243https://repositorio.ufmg.br/bitstream/1843/56331/2/Characterization%20of%20Aspergillus%20fumigatus%20Extracellular%20Vesicles%20and%20Their%20Effects%20on%20Macrophages%20and%20Neutrophils%20Functions.pdf0c885454269667cacd42ec117249664bMD521843/563312023-07-14 19:45:34.662oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-07-14T22:45:34Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Characterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functions |
title |
Characterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functions |
spellingShingle |
Characterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functions Jéssica Amanda Marques Souza Extracellular vesicles Flamentous fungus Aspergillus fumigatus Host-pathogen interactions Macrophages Neutrophils Aspergillus fumigatus Macrófagos Neutrofilos |
title_short |
Characterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functions |
title_full |
Characterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functions |
title_fullStr |
Characterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functions |
title_full_unstemmed |
Characterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functions |
title_sort |
Characterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functions |
author |
Jéssica Amanda Marques Souza |
author_facet |
Jéssica Amanda Marques Souza Frédéric Jean Georges Frézard Daniele da Glória de Souza Mauro Martins Teixeira Frederico Marianetti Soriani Ludmila de Matos Baltazar Virgínia Mendes Carregal Ludmila Gouveia Eufrasio André Gustavo de Oliveira Wendell Girard Dias Kildare Rocha de Miranda Iran Malavazi Daniel de Assis Santos |
author_role |
author |
author2 |
Frédéric Jean Georges Frézard Daniele da Glória de Souza Mauro Martins Teixeira Frederico Marianetti Soriani Ludmila de Matos Baltazar Virgínia Mendes Carregal Ludmila Gouveia Eufrasio André Gustavo de Oliveira Wendell Girard Dias Kildare Rocha de Miranda Iran Malavazi Daniel de Assis Santos |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Jéssica Amanda Marques Souza Frédéric Jean Georges Frézard Daniele da Glória de Souza Mauro Martins Teixeira Frederico Marianetti Soriani Ludmila de Matos Baltazar Virgínia Mendes Carregal Ludmila Gouveia Eufrasio André Gustavo de Oliveira Wendell Girard Dias Kildare Rocha de Miranda Iran Malavazi Daniel de Assis Santos |
dc.subject.por.fl_str_mv |
Extracellular vesicles Flamentous fungus Aspergillus fumigatus Host-pathogen interactions Macrophages Neutrophils |
topic |
Extracellular vesicles Flamentous fungus Aspergillus fumigatus Host-pathogen interactions Macrophages Neutrophils Aspergillus fumigatus Macrófagos Neutrofilos |
dc.subject.other.pt_BR.fl_str_mv |
Aspergillus fumigatus Macrófagos Neutrofilos |
description |
Extracellular vesicles (EVs) has been considered an alternative process for intercellular communication. EVs release by filamentous fungi and the role of vesicular secretion during fungus-host cells interaction remain unknown. Here, we identified the secretion of EVs from the pathogenic filamentous fungus, Aspergillus fumigatus. Analysis of the structure of EVs demonstrated that A. fumigatus produces round shaped bilayer structures ranging from 100 to 200 nm size, containing ergosterol and a myriad of proteins involved in REDOX, cell wall remodeling and metabolic functions of the fungus. We demonstrated that macrophages can phagocytose A. fumigatus EVs. Phagocytic cells, stimulated with EVs, increased fungal clearance after A. fumigatus conidia challenge. EVs were also able to induce the production of TNF-α and CCL2 by macrophages and a synergistic effect was observed in the production of these mediators when the cells were challenged with the conidia. In bone marrow-derived neutrophils (BMDN) treated with EVs, there was enhancement of the production of TNF-α and IL-1β in response to conidia. Together, our results demonstrate, for the first time, that A. fumigatus produces EVs containing a diverse set of proteins involved in fungal physiology and virulence. Moreover, EVs are biologically active and stimulate production of inflammatory mediators and fungal clearance. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2023-07-14T22:45:34Z |
dc.date.available.fl_str_mv |
2023-07-14T22:45:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/56331 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.3389/fmicb.2019.02008 |
dc.identifier.issn.pt_BR.fl_str_mv |
1664-302X |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0003-3783-5717 https://orcid.org/0000-0002-6944-3008 https://orcid.org/0000-0003-4720-6746 https://orcid.org/0000-0002-4255-3047 https://orcid.org/0000-0001-9152-8756 https://orcid.org/0000-0001-8186-8714 https://orcid.org/0000-0001-9799-773X https://orcid.org/0000-0002-1108-5666 |
url |
https://doi.org/10.3389/fmicb.2019.02008 http://hdl.handle.net/1843/56331 https://orcid.org/0000-0003-3783-5717 https://orcid.org/0000-0002-6944-3008 https://orcid.org/0000-0003-4720-6746 https://orcid.org/0000-0002-4255-3047 https://orcid.org/0000-0001-9152-8756 https://orcid.org/0000-0001-8186-8714 https://orcid.org/0000-0001-9799-773X https://orcid.org/0000-0002-1108-5666 |
identifier_str_mv |
1664-302X |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Frontiers in Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICB - DEPARTAMENTO DE FARMACOLOGIA ICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA ICB - DEPARTAMENTO DE MICROBIOLOGIA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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