Auxin production by endophytic bacteria isolated from banana trees

Detalhes bibliográficos
Autor(a) principal: Izabela Cristina Pires Gomes
Data de Publicação: 2017
Outros Autores: Amanda Dayane Malta Matos, Silvia Nietsche, Adelica Aparecida Xavier, Márcia Regina Costa, Wellington Silva Gomes, Marlon Cristian Toledo Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: http://hdl.handle.net/1843/46093
Resumo: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
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spelling 2022-10-07T15:22:07Z2022-10-07T15:22:07Z201760e1716048410.1590/1678-4324-20171604841678-4324http://hdl.handle.net/1843/46093CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorThis study aimed to evaluate the capacity for in vitro indole compound synthesis by endophytic bacteria and tocompare the Salkowski colorimetric and high-performance liquid chromatography (HPLC) quantification methods. Initial screenings were performed using 10% tryptic soy agar (TSA) medium with and without L-tryptophan via theSalkowski colorimetric method in a completely randomized design with 40 treatments and three replications. In thesecond experiment, the five most promising isolates were used for the detection and quantification of indoles by HPLC in a completely randomized design with five treatments and three replications. Of the 40 isolates, 16synthesized indole-3-acetic acid (IAA) in the presence of L-tryptophan, and only three synthesized IAA in theabsence of this amino acid. The concentration of IAA determined by absorbance indicated that Agrobacteriumtumefaciens isolate EB.07 showed a higher concentration of IAA in the presence of L-tryptophan (16.61 μ g mL -1 ). HPLC analysis revealed the production of the following four indole compounds by these bacteria: IAA, indole pyruvic acid (IPA), indole lactic acid (ILA), and indole-3-acetaldehyde (IAAld). Significant differences between thetwo estimation methods were observed for two of the five tested isolates: A. tumefaciens (EB.07) and Bacillus pumilus (EB.12). For both isolates, the Salkowski method indicated higher concentrations than those obtained with HPLC. Considering the obtained results, the Salkowski colorimetric method should be used in initial screenings,and HPLC should be used as a complementary method in the selection of isolates with the highest potential tosynthesize indoles.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASBrazilian Archives of Biology and TechnologyPlantas - Efeito da auxinaAlcalóides indólicosAIABacillus spp.HPLCcolorimetriaAuxin production by endophytic bacteria isolated from banana treesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleIzabela Cristina Pires GomesAmanda Dayane Malta MatosSilvia NietscheAdelica Aparecida XavierMárcia Regina CostaWellington Silva GomesMarlon Cristian Toledo Pereirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/46093/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALAuxin production by endophytic bacteria isolated from banana trees.pdfAuxin production by endophytic bacteria isolated from banana trees.pdfapplication/pdf1172458https://repositorio.ufmg.br/bitstream/1843/46093/2/Auxin%20production%20by%20endophytic%20bacteria%20isolated%20from%20banana%20trees.pdf4ed400ede3d4e801b54ba525603f23f9MD521843/460932022-10-07 12:22:07.3oai:repositorio.ufmg.br:1843/46093TElDRU7vv71BIERFIERJU1RSSUJVSe+/ve+/vU8gTu+/vU8tRVhDTFVTSVZBIERPIFJFUE9TSVTvv71SSU8gSU5TVElUVUNJT05BTCBEQSBVRk1HCiAKCkNvbSBhIGFwcmVzZW50Ye+/ve+/vW8gZGVzdGEgbGljZW7vv71hLCB2b2Pvv70gKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIGFvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIChSSS1VRk1HKSBvIGRpcmVpdG8gbu+/vW8gZXhjbHVzaXZvIGUgaXJyZXZvZ++/vXZlbCBkZSByZXByb2R1emlyIGUvb3UgZGlzdHJpYnVpciBhIHN1YSBwdWJsaWNh77+977+9byAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0cu+/vW5pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mg77+9dWRpbyBvdSB277+9ZGVvLgoKVm9j77+9IGRlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zvv710aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRvIHNldSBkb2N1bWVudG8gZSBxdWUgY29uaGVjZSBlIGFjZWl0YSBhcyBEaXJldHJpemVzIGRvIFJJLVVGTUcuCgpWb2Pvv70gY29uY29yZGEgcXVlIG8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVGTUcgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250Ze+/vWRvLCB0cmFuc3BvciBhIHN1YSBwdWJsaWNh77+977+9byBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHvv73vv71vLgoKVm9j77+9IHRhbWLvv71tIGNvbmNvcmRhIHF1ZSBvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPvv71waWEgZGUgc3VhIHB1YmxpY2Hvv73vv71vIHBhcmEgZmlucyBkZSBzZWd1cmFu77+9YSwgYmFjay11cCBlIHByZXNlcnZh77+977+9by4KClZvY++/vSBkZWNsYXJhIHF1ZSBhIHN1YSBwdWJsaWNh77+977+9byDvv70gb3JpZ2luYWwgZSBxdWUgdm9j77+9IHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vu77+9YS4gVm9j77+9IHRhbWLvv71tIGRlY2xhcmEgcXVlIG8gZGVw77+9c2l0byBkZSBzdWEgcHVibGljYe+/ve+/vW8gbu+/vW8sIHF1ZSBzZWphIGRlIHNldSBjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd177+9bS4KCkNhc28gYSBzdWEgcHVibGljYe+/ve+/vW8gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY++/vSBu77+9byBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2Pvv70gZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc++/vW8gaXJyZXN0cml0YSBkbyBkZXRlbnRvciBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgcGFyYSBjb25jZWRlciBhbyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7vv71hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Tvv70gY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250Ze+/vWRvIGRhIHB1YmxpY2Hvv73vv71vIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFBVQkxJQ0Hvv73vv71PIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ++/vU5JTyBPVSBBUE9JTyBERSBVTUEgQUfvv71OQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PLCBWT0Pvv70gREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklT77+9TyBDT01PIFRBTULvv71NIEFTIERFTUFJUyBPQlJJR0Hvv73vv71FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKTyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lKHMpIG91IG8ocykgbm9tZXMocykgZG8ocykgZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSBwdWJsaWNh77+977+9bywgZSBu77+9byBmYXLvv70gcXVhbHF1ZXIgYWx0ZXJh77+977+9bywgYWzvv71tIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7vv71hLgo=Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-10-07T15:22:07Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv Auxin production by endophytic bacteria isolated from banana trees
title Auxin production by endophytic bacteria isolated from banana trees
spellingShingle Auxin production by endophytic bacteria isolated from banana trees
Izabela Cristina Pires Gomes
AIA
Bacillus spp.
HPLC
colorimetria
Plantas - Efeito da auxina
Alcalóides indólicos
title_short Auxin production by endophytic bacteria isolated from banana trees
title_full Auxin production by endophytic bacteria isolated from banana trees
title_fullStr Auxin production by endophytic bacteria isolated from banana trees
title_full_unstemmed Auxin production by endophytic bacteria isolated from banana trees
title_sort Auxin production by endophytic bacteria isolated from banana trees
author Izabela Cristina Pires Gomes
author_facet Izabela Cristina Pires Gomes
Amanda Dayane Malta Matos
Silvia Nietsche
Adelica Aparecida Xavier
Márcia Regina Costa
Wellington Silva Gomes
Marlon Cristian Toledo Pereira
author_role author
author2 Amanda Dayane Malta Matos
Silvia Nietsche
Adelica Aparecida Xavier
Márcia Regina Costa
Wellington Silva Gomes
Marlon Cristian Toledo Pereira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Izabela Cristina Pires Gomes
Amanda Dayane Malta Matos
Silvia Nietsche
Adelica Aparecida Xavier
Márcia Regina Costa
Wellington Silva Gomes
Marlon Cristian Toledo Pereira
dc.subject.por.fl_str_mv AIA
Bacillus spp.
HPLC
colorimetria
topic AIA
Bacillus spp.
HPLC
colorimetria
Plantas - Efeito da auxina
Alcalóides indólicos
dc.subject.other.pt_BR.fl_str_mv Plantas - Efeito da auxina
Alcalóides indólicos
description CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2022-10-07T15:22:07Z
dc.date.available.fl_str_mv 2022-10-07T15:22:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/46093
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/1678-4324-2017160484
dc.identifier.issn.pt_BR.fl_str_mv 1678-4324
identifier_str_mv 10.1590/1678-4324-2017160484
1678-4324
url http://hdl.handle.net/1843/46093
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Brazilian Archives of Biology and Technology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
bitstream.url.fl_str_mv https://repositorio.ufmg.br/bitstream/1843/46093/1/License.txt
https://repositorio.ufmg.br/bitstream/1843/46093/2/Auxin%20production%20by%20endophytic%20bacteria%20isolated%20from%20banana%20trees.pdf
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repository.name.fl_str_mv Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv
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