Bovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone loss
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.3389/fbioe.2020.00891 http://hdl.handle.net/1843/53961 https://orcid.org/0000-0002-7423-9802 https://orcid.org/0000-0001-7171-3145 https://orcid.org/0000-0002-2839-8757 https://orcid.org/0000-0003-1052-0854 https://orcid.org/0000-0002-6944-3008 https://orcid.org/0000-0003-2256-8652 https://orcid.org/0000-0002-5851-099X |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2023-05-25T20:15:57Z2023-05-25T20:15:57Z20208https://doi.org/10.3389/fbioe.2020.008912296-4185http://hdl.handle.net/1843/53961https://orcid.org/0000-0002-7423-9802https://orcid.org/0000-0001-7171-3145https://orcid.org/0000-0002-2839-8757https://orcid.org/0000-0003-1052-0854https://orcid.org/0000-0002-6944-3008https://orcid.org/0000-0003-2256-8652https://orcid.org/0000-0002-5851-099XCNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorStudying effects of milk components on bone may have a clinical impact as milk is highly associated with bone maintenance, and clinical studies provided controversial associations with dairy consumption. We aimed to evaluate the impact of milk extracellular vesicles (mEVs) on the dynamics of bone loss in mice. MEVs are nanoparticles containing proteins, mRNA and microRNA, and were supplemented into the drinking water of mice, either receiving diet-induced obesity or ovariectomy (OVX). Mice receiving mEVs were protected from the bone loss caused by diet-induced obesity. In a more severe model of bone loss, OVX, higher osteoclast numbers in the femur were found, which were lowered by mEV treatment. Additionally, the osteoclastogenic potential of bone marrow-derived precursor cells was lowered in mEV-treated mice. The reduced stiffness in the femur of OVX mice was consequently reversed by mEV treatment, accompanied by improvement in the bone microarchitecture. In general, the RANKL/OPG ratio increased systemically and locally in both models and was rescued by mEV treatment. The number of osteocytes, as primary regulators of the RANKL/OPG system, raised in the femur of the OVX mEVs-treated group compared to OVX nontreated mice. Also, the osteocyte cell line treated with mEVs demonstrated a lowered RANKL/OPG ratio. Thus, mEVs showed systemic and local osteoprotective properties in two mouse models of bone loss reflected in reduced osteoclast presence. Data reveal mEV potential in bone modulation, acting via osteocyte enhancement and RANKL/OPG regulation. We suggest that mEVs could be a therapeutic candidate for the treatment of bone loss.porUniversidade Federal de Minas GeraisUFMGBrasilENF - DEPARTAMENTO DE NUTRIÇÃOFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORAICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIAICB - DEPARTAMENTO DE FARMACOLOGIAFrontiers in bioengineering and biotechnologyVesículas extracelularesLeiteOsteoporoseObesidadeOsteócitosextracellular vesiclesmilkbone lossosteoporosisobesityosteocyteBovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone lossinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.frontiersin.org/articles/10.3389/fbioe.2020.00891/fullMarina Chaves de OliveiraBartijn PietersPolianna B. GuimarãesLetícia F. DufflesJoyce Elisa HerediaAna Letícia Malheiros SilveiraAmanda C. C. OliveiraMauro Martins TeixeiraAdaliene Versiani Matos FerreiraTarcilia A SilvaFons Van De LooSoraia Macariapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/53961/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALBovine Milk Extracellular Vesicles Are Osteoprotective by Increasing Osteocyte Numbers and Targeting RANKL_OPG System in Experimental Models of Bone Loss.pdfBovine Milk Extracellular Vesicles Are Osteoprotective by Increasing Osteocyte Numbers and Targeting RANKL_OPG System in Experimental Models of Bone Loss.pdfapplication/pdf26149010https://repositorio.ufmg.br/bitstream/1843/53961/2/Bovine%20Milk%20Extracellular%20Vesicles%20Are%20Osteoprotective%20by%20Increasing%20Osteocyte%20Numbers%20and%20Targeting%20RANKL_OPG%20System%20in%20Experimental%20Models%20of%20Bone%20Loss.pdffe1721a00b2ae0c6a050981dfc427e3eMD521843/539612023-05-25 17:15:57.611oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-05-25T20:15:57Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Bovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone loss |
| title |
Bovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone loss |
| spellingShingle |
Bovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone loss Marina Chaves de Oliveira extracellular vesicles milk bone loss osteoporosis obesity osteocyte Vesículas extracelulares Leite Osteoporose Obesidade Osteócitos |
| title_short |
Bovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone loss |
| title_full |
Bovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone loss |
| title_fullStr |
Bovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone loss |
| title_full_unstemmed |
Bovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone loss |
| title_sort |
Bovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone loss |
| author |
Marina Chaves de Oliveira |
| author_facet |
Marina Chaves de Oliveira Bartijn Pieters Polianna B. Guimarães Letícia F. Duffles Joyce Elisa Heredia Ana Letícia Malheiros Silveira Amanda C. C. Oliveira Mauro Martins Teixeira Adaliene Versiani Matos Ferreira Tarcilia A Silva Fons Van De Loo Soraia Macari |
| author_role |
author |
| author2 |
Bartijn Pieters Polianna B. Guimarães Letícia F. Duffles Joyce Elisa Heredia Ana Letícia Malheiros Silveira Amanda C. C. Oliveira Mauro Martins Teixeira Adaliene Versiani Matos Ferreira Tarcilia A Silva Fons Van De Loo Soraia Macari |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Marina Chaves de Oliveira Bartijn Pieters Polianna B. Guimarães Letícia F. Duffles Joyce Elisa Heredia Ana Letícia Malheiros Silveira Amanda C. C. Oliveira Mauro Martins Teixeira Adaliene Versiani Matos Ferreira Tarcilia A Silva Fons Van De Loo Soraia Macari |
| dc.subject.por.fl_str_mv |
extracellular vesicles milk bone loss osteoporosis obesity osteocyte |
| topic |
extracellular vesicles milk bone loss osteoporosis obesity osteocyte Vesículas extracelulares Leite Osteoporose Obesidade Osteócitos |
| dc.subject.other.pt_BR.fl_str_mv |
Vesículas extracelulares Leite Osteoporose Obesidade Osteócitos |
| description |
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| publishDate |
2020 |
| dc.date.issued.fl_str_mv |
2020 |
| dc.date.accessioned.fl_str_mv |
2023-05-25T20:15:57Z |
| dc.date.available.fl_str_mv |
2023-05-25T20:15:57Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/1843/53961 |
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https://doi.org/10.3389/fbioe.2020.00891 |
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2296-4185 |
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https://orcid.org/0000-0002-7423-9802 https://orcid.org/0000-0001-7171-3145 https://orcid.org/0000-0002-2839-8757 https://orcid.org/0000-0003-1052-0854 https://orcid.org/0000-0002-6944-3008 https://orcid.org/0000-0003-2256-8652 https://orcid.org/0000-0002-5851-099X |
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https://doi.org/10.3389/fbioe.2020.00891 http://hdl.handle.net/1843/53961 https://orcid.org/0000-0002-7423-9802 https://orcid.org/0000-0001-7171-3145 https://orcid.org/0000-0002-2839-8757 https://orcid.org/0000-0003-1052-0854 https://orcid.org/0000-0002-6944-3008 https://orcid.org/0000-0003-2256-8652 https://orcid.org/0000-0002-5851-099X |
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2296-4185 |
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por |
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por |
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Frontiers in bioengineering and biotechnology |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ENF - DEPARTAMENTO DE NUTRIÇÃO FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICB - DEPARTAMENTO DE FARMACOLOGIA |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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