Epithelial–mesenchymal transition inhibition by metformin reduces melanoma lung metastasis in a murine model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | https://doi.org/10.1038/s41598-022-22235-8 http://hdl.handle.net/1843/60398 https://orcid.org/0000-0002-1835-0303 |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2023-10-31T21:36:30Z2023-10-31T21:36:30Z2022-10-221211777613https://doi.org/10.1038/s41598-022-22235-82045-2322http://hdl.handle.net/1843/60398https://orcid.org/0000-0002-1835-0303CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorMelanoma is an aggressive cancer with fast metastatic spread and reduced survival time. One common event during the neoplastic progression is the epithelial–mesenchymal transition (EMT), which enhances invasiveness, cell migration, and metastasis. In this study, we investigated the effects of metformin at EMT in melanoma cell lines B16-F10 and A-375, in vitro, and the impact of EMT downregulation on melanoma progression in vivo. The metformin cells treatment reduces the migration potential in vitro and reduced the development of pulmonary metastases and the expressions of N-cadherin, vimentin, ZEB1, and ZEB2 at the metastases site, in vivo. These results indicate that metformin can promote EMT downregulation impairing the metastatic potential of melanoma cells.engUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE PATOLOGIAICB - INSTITUTO DE CIÊNCIAS BIOLOGICASScientific ReportsMetástaseTumoresMelanomaMetástaseTumoresMelanomaEpithelial–mesenchymal transition inhibition by metformin reduces melanoma lung metastasis in a murine modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.nature.com/articles/s41598-022-22235-8#rightslinkEmerson Soares VelosoHelen Lima Del PuertoEnio FerreiraBárbara Andrade de CarvalhoFelipe Henrique de Souza SilvaThaís Salviana RibeiroBruna Mendes LimaCamila Pereira AlmeidaVítor Henrique Soares Romão da SilvaSara Aparecida RochaMarina Rios de Araújo Camposapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/60398/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALEpithelial–mesenchymal transition inhibition by metformin reduces melanoma lung metastasis in a murine model.pdfEpithelial–mesenchymal transition inhibition by metformin reduces melanoma lung metastasis in a murine model.pdfapplication/pdf3054399https://repositorio.ufmg.br/bitstream/1843/60398/2/Epithelial%e2%80%93mesenchymal%20transition%20inhibition%20by%20metformin%20reduces%20melanoma%20lung%20metastasis%20in%20a%20murine%20model.pdfc7afa07fdfd0363ce31ef765a34372bdMD521843/603982023-10-31 18:36:30.955oai:repositorio.ufmg.br:1843/60398TElDRU7vv71BIERFIERJU1RSSUJVSe+/ve+/vU8gTu+/vU8tRVhDTFVTSVZBIERPIFJFUE9TSVTvv71SSU8gSU5TVElUVUNJT05BTCBEQSBVRk1HCiAKCkNvbSBhIGFwcmVzZW50Ye+/ve+/vW8gZGVzdGEgbGljZW7vv71hLCB2b2Pvv70gKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIGFvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIChSSS1VRk1HKSBvIGRpcmVpdG8gbu+/vW8gZXhjbHVzaXZvIGUgaXJyZXZvZ++/vXZlbCBkZSByZXByb2R1emlyIGUvb3UgZGlzdHJpYnVpciBhIHN1YSBwdWJsaWNh77+977+9byAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0cu+/vW5pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mg77+9dWRpbyBvdSB277+9ZGVvLgoKVm9j77+9IGRlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zvv710aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRvIHNldSBkb2N1bWVudG8gZSBxdWUgY29uaGVjZSBlIGFjZWl0YSBhcyBEaXJldHJpemVzIGRvIFJJLVVGTUcuCgpWb2Pvv70gY29uY29yZGEgcXVlIG8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVGTUcgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250Ze+/vWRvLCB0cmFuc3BvciBhIHN1YSBwdWJsaWNh77+977+9byBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHvv73vv71vLgoKVm9j77+9IHRhbWLvv71tIGNvbmNvcmRhIHF1ZSBvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPvv71waWEgZGUgc3VhIHB1YmxpY2Hvv73vv71vIHBhcmEgZmlucyBkZSBzZWd1cmFu77+9YSwgYmFjay11cCBlIHByZXNlcnZh77+977+9by4KClZvY++/vSBkZWNsYXJhIHF1ZSBhIHN1YSBwdWJsaWNh77+977+9byDvv70gb3JpZ2luYWwgZSBxdWUgdm9j77+9IHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vu77+9YS4gVm9j77+9IHRhbWLvv71tIGRlY2xhcmEgcXVlIG8gZGVw77+9c2l0byBkZSBzdWEgcHVibGljYe+/ve+/vW8gbu+/vW8sIHF1ZSBzZWphIGRlIHNldSBjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd177+9bS4KCkNhc28gYSBzdWEgcHVibGljYe+/ve+/vW8gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY++/vSBu77+9byBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2Pvv70gZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc++/vW8gaXJyZXN0cml0YSBkbyBkZXRlbnRvciBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgcGFyYSBjb25jZWRlciBhbyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7vv71hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Tvv70gY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250Ze+/vWRvIGRhIHB1YmxpY2Hvv73vv71vIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFBVQkxJQ0Hvv73vv71PIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ++/vU5JTyBPVSBBUE9JTyBERSBVTUEgQUfvv71OQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PLCBWT0Pvv70gREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklT77+9TyBDT01PIFRBTULvv71NIEFTIERFTUFJUyBPQlJJR0Hvv73vv71FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKTyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lKHMpIG91IG8ocykgbm9tZXMocykgZG8ocykgZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSBwdWJsaWNh77+977+9bywgZSBu77+9byBmYXLvv70gcXVhbHF1ZXIgYWx0ZXJh77+977+9bywgYWzvv71tIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7vv71hLgo=Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-10-31T21:36:30Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
Epithelial–mesenchymal transition inhibition by metformin reduces melanoma lung metastasis in a murine model |
| title |
Epithelial–mesenchymal transition inhibition by metformin reduces melanoma lung metastasis in a murine model |
| spellingShingle |
Epithelial–mesenchymal transition inhibition by metformin reduces melanoma lung metastasis in a murine model Emerson Soares Veloso Metástase Tumores Melanoma Metástase Tumores Melanoma |
| title_short |
Epithelial–mesenchymal transition inhibition by metformin reduces melanoma lung metastasis in a murine model |
| title_full |
Epithelial–mesenchymal transition inhibition by metformin reduces melanoma lung metastasis in a murine model |
| title_fullStr |
Epithelial–mesenchymal transition inhibition by metformin reduces melanoma lung metastasis in a murine model |
| title_full_unstemmed |
Epithelial–mesenchymal transition inhibition by metformin reduces melanoma lung metastasis in a murine model |
| title_sort |
Epithelial–mesenchymal transition inhibition by metformin reduces melanoma lung metastasis in a murine model |
| author |
Emerson Soares Veloso |
| author_facet |
Emerson Soares Veloso Helen Lima Del Puerto Enio Ferreira Bárbara Andrade de Carvalho Felipe Henrique de Souza Silva Thaís Salviana Ribeiro Bruna Mendes Lima Camila Pereira Almeida Vítor Henrique Soares Romão da Silva Sara Aparecida Rocha Marina Rios de Araújo Campos |
| author_role |
author |
| author2 |
Helen Lima Del Puerto Enio Ferreira Bárbara Andrade de Carvalho Felipe Henrique de Souza Silva Thaís Salviana Ribeiro Bruna Mendes Lima Camila Pereira Almeida Vítor Henrique Soares Romão da Silva Sara Aparecida Rocha Marina Rios de Araújo Campos |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Emerson Soares Veloso Helen Lima Del Puerto Enio Ferreira Bárbara Andrade de Carvalho Felipe Henrique de Souza Silva Thaís Salviana Ribeiro Bruna Mendes Lima Camila Pereira Almeida Vítor Henrique Soares Romão da Silva Sara Aparecida Rocha Marina Rios de Araújo Campos |
| dc.subject.por.fl_str_mv |
Metástase Tumores Melanoma |
| topic |
Metástase Tumores Melanoma Metástase Tumores Melanoma |
| dc.subject.other.pt_BR.fl_str_mv |
Metástase Tumores Melanoma |
| description |
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| publishDate |
2022 |
| dc.date.issued.fl_str_mv |
2022-10-22 |
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2023-10-31T21:36:30Z |
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2023-10-31T21:36:30Z |
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info:eu-repo/semantics/publishedVersion |
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http://hdl.handle.net/1843/60398 |
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https://doi.org/10.1038/s41598-022-22235-8 |
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2045-2322 |
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https://orcid.org/0000-0002-1835-0303 |
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Universidade Federal de Minas Gerais |
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Brasil |
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ICB - DEPARTAMENTO DE PATOLOGIA ICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS |
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Universidade Federal de Minas Gerais |
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