Proteomic profile of Brucella abortus-infected bovine chorioallantoic membrane explants

• Autor(a) principal: Juliana Pinto da Silva Mol
• Data de Publicação: 2016
• Outros Autores: Simone da Fonseca Pires, Donat Alexander de Chapeaurouge, Jonas Enrique Aguilar Perales, Renato de Lima Santos, Hélida Monteiro de Andrade, Andrey Pereira Lage
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: https://doi.org/10.1371/journal.pone.0154209; http://hdl.handle.net/1843/56287; https://orcid.org/0000-0002-6946-8213; https://orcid.org/0000-0001-8706-9243; https://orcid.org/0000-0002-0157-1783; https://orcid.org/0000-0002-4830-0470; https://orcid.org/0000-0003-2823-4530; https://orcid.org/0000-0002-9325-5728
• topic: Brucella abortus; Bovine brucellosis; Bovine chorioallantoic membrane; Inflammation; Brucella abortus; Brucelose bovina; Bovino - Infecções; Bovino - Doenças; Brucelose em bovino; Aborto nos animais

Brucella abortus is the etiological agent of bovine brucellosis, a zoonotic disease that causes significant economic losses worldwide. The differential proteomic profile of bovine chorioallantoic membrane (CAM) explants at early stages of infection with B. abortus (0.5, 2, 4, and 8 h) was determined. Analysis of CAM explants at 0.5 and 4 h showed the highest differences between uninfected and infected CAM explants, and therefore were used for the Differential Gel Electrophoresis (DIGE). A total of 103 spots were present in only one experimental group and were selected for identification by mass spectrometry (MALDI/ToFToF). Proteins only identified in extracts of CAM explants infected with B. abortus were related to recognition of PAMPs by TLR, production of reactive oxygen species, intracellular trafficking, and inflammation.