Antagonistic effect of trichoderma isolates and its metabolites against fusarium solani and f. oxysporum in chickpea
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Publication Date: | 2020 |
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Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UFMG |
Download full: | http://hdl.handle.net/1843/41678 |
Summary: | The aim of this work was to study and select Trichoderma sp. strains with biocontrol potential against Fusarium solani and F. oxysporum, the causal agents of root rot and wilt in chickpea. Antagonism against pathogenic isolates of F. solani and F. oxysporum of twenty-one isolates of Trichoderma sp. obtained from the rhizosphere of chickpea plants were evaluated in vitro through competition in dual culture tests as well as the production of volatile and non-volatile organic compounds exhibiting fungicidal and/or fungistatic activity. In the in vivo experiment, four isolates of Trichoderma sp. were selected and their antagonism was evaluated separately and combined with a commercial product based on T. asperellum under greenhouse conditions. Trichoderma sp. isolates were efficient competitors and produced metabolites capable of inhibiting mycelial growth of both species of Fusarium. Our results show the great versatility of the mechanisms of action from Trichoderma isolates, mainly associated with the production of volatile organic compounds. Despite the antagonistic effect of Trichoderma isolates observed in vitro, these isolates did not control Fusarium neither promote chickpea growth in in vivo conditions. |
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2022-05-13T22:19:24Z2022-05-13T22:19:24Z202066363443636110.34117/bjdv6n6-2512525-8761http://hdl.handle.net/1843/41678The aim of this work was to study and select Trichoderma sp. strains with biocontrol potential against Fusarium solani and F. oxysporum, the causal agents of root rot and wilt in chickpea. Antagonism against pathogenic isolates of F. solani and F. oxysporum of twenty-one isolates of Trichoderma sp. obtained from the rhizosphere of chickpea plants were evaluated in vitro through competition in dual culture tests as well as the production of volatile and non-volatile organic compounds exhibiting fungicidal and/or fungistatic activity. In the in vivo experiment, four isolates of Trichoderma sp. were selected and their antagonism was evaluated separately and combined with a commercial product based on T. asperellum under greenhouse conditions. Trichoderma sp. isolates were efficient competitors and produced metabolites capable of inhibiting mycelial growth of both species of Fusarium. Our results show the great versatility of the mechanisms of action from Trichoderma isolates, mainly associated with the production of volatile organic compounds. Despite the antagonistic effect of Trichoderma isolates observed in vitro, these isolates did not control Fusarium neither promote chickpea growth in in vivo conditions.O objetivo deste trabalho foi estudar e selecionar Trichoderma sp. cepas com potencial de biocontrole contra Fusarium solani e F. oxysporum, agentes causadores da podridão radicular e murcha em grão-de-bico. Antagonismo contra isolados patogênicos de F. solani e F. oxysporum de vinte e um isolados de Trichoderma sp. obtidos da rizosfera de plantas de grão-de-bico foram avaliados in vitro por meio de testes de competição em dupla cultura, bem como a produção de compostos orgânicos voláteis e não voláteis com atividade fungicida e/ou fungistática. No experimento in vivo, quatro isolados de Trichoderma sp. foram selecionados e seu antagonismo foi avaliado separadamente e combinado com um produto comercial à base de T. asperellum em casa de vegetação. Trichoderma sp. isolados foram competidores eficientes e produziram metabólitos capazes de inibir o crescimento micelial de ambas as espécies de Fusarium. Nossos resultados mostram a grande versatilidade dos mecanismos de ação dos isolados de Trichoderma, principalmente associados à produção de compostos orgânicos voláteis. Apesar do efeito antagônico dos isolados de Trichoderma observado in vitro, esses isolados não controlaram Fusarium nem promoveram o crescimento do grão de bico em condições in vivo.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASBrazilian journal of developmentCicer arietinumControle biológicoMetabólitos secundáriosFusarioseCicer arietinumBiological controlFusariosisSecondary metabolitesAntagonistic effect of trichoderma isolates and its metabolites against fusarium solani and f. oxysporum in chickpeaEfeito antagônico de isolados de tricoderma e seus metabólitos contra Fusarium solani e f. oxysporum em grão de bicoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.brazilianjournals.com/index.php/BRJD/article/view/11481Daiana Maria QueirozazevedoFernando da Silva RochaMaria de Fátima Gonçalves FernandesCândido Alves CostaMaria de Fátima Silva MunizPatrícia Döerl BarrosoFernanda Letycia AmaralDandara Maria Clara do Rosário Barbosaapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/41678/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINAL2020_Antagonistic effect of trichoderma isolates and its metabolites against fusarium solani and f. oxysporum in chickpea.pdf2020_Antagonistic effect of trichoderma isolates and its metabolites against fusarium solani and f. oxysporum in chickpea.pdfapplication/pdf705912https://repositorio.ufmg.br/bitstream/1843/41678/2/2020_Antagonistic%20effect%20of%20trichoderma%20isolates%20and%20its%20metabolites%20against%20fusarium%20solani%20and%20f.%20oxysporum%20in%20chickpea.pdfa612510bb8b8bd890760ee036a80320eMD521843/416782022-05-13 19:19:24.366oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-05-13T22:19:24Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Antagonistic effect of trichoderma isolates and its metabolites against fusarium solani and f. oxysporum in chickpea |
dc.title.alternative.pt_BR.fl_str_mv |
Efeito antagônico de isolados de tricoderma e seus metabólitos contra Fusarium solani e f. oxysporum em grão de bico |
title |
Antagonistic effect of trichoderma isolates and its metabolites against fusarium solani and f. oxysporum in chickpea |
spellingShingle |
Antagonistic effect of trichoderma isolates and its metabolites against fusarium solani and f. oxysporum in chickpea Daiana Maria Queirozazevedo Cicer arietinum Biological control Fusariosis Secondary metabolites Cicer arietinum Controle biológico Metabólitos secundários Fusariose |
title_short |
Antagonistic effect of trichoderma isolates and its metabolites against fusarium solani and f. oxysporum in chickpea |
title_full |
Antagonistic effect of trichoderma isolates and its metabolites against fusarium solani and f. oxysporum in chickpea |
title_fullStr |
Antagonistic effect of trichoderma isolates and its metabolites against fusarium solani and f. oxysporum in chickpea |
title_full_unstemmed |
Antagonistic effect of trichoderma isolates and its metabolites against fusarium solani and f. oxysporum in chickpea |
title_sort |
Antagonistic effect of trichoderma isolates and its metabolites against fusarium solani and f. oxysporum in chickpea |
author |
Daiana Maria Queirozazevedo |
author_facet |
Daiana Maria Queirozazevedo Fernando da Silva Rocha Maria de Fátima Gonçalves Fernandes Cândido Alves Costa Maria de Fátima Silva Muniz Patrícia Döerl Barroso Fernanda Letycia Amaral Dandara Maria Clara do Rosário Barbosa |
author_role |
author |
author2 |
Fernando da Silva Rocha Maria de Fátima Gonçalves Fernandes Cândido Alves Costa Maria de Fátima Silva Muniz Patrícia Döerl Barroso Fernanda Letycia Amaral Dandara Maria Clara do Rosário Barbosa |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Daiana Maria Queirozazevedo Fernando da Silva Rocha Maria de Fátima Gonçalves Fernandes Cândido Alves Costa Maria de Fátima Silva Muniz Patrícia Döerl Barroso Fernanda Letycia Amaral Dandara Maria Clara do Rosário Barbosa |
dc.subject.por.fl_str_mv |
Cicer arietinum Biological control Fusariosis Secondary metabolites |
topic |
Cicer arietinum Biological control Fusariosis Secondary metabolites Cicer arietinum Controle biológico Metabólitos secundários Fusariose |
dc.subject.other.pt_BR.fl_str_mv |
Cicer arietinum Controle biológico Metabólitos secundários Fusariose |
description |
The aim of this work was to study and select Trichoderma sp. strains with biocontrol potential against Fusarium solani and F. oxysporum, the causal agents of root rot and wilt in chickpea. Antagonism against pathogenic isolates of F. solani and F. oxysporum of twenty-one isolates of Trichoderma sp. obtained from the rhizosphere of chickpea plants were evaluated in vitro through competition in dual culture tests as well as the production of volatile and non-volatile organic compounds exhibiting fungicidal and/or fungistatic activity. In the in vivo experiment, four isolates of Trichoderma sp. were selected and their antagonism was evaluated separately and combined with a commercial product based on T. asperellum under greenhouse conditions. Trichoderma sp. isolates were efficient competitors and produced metabolites capable of inhibiting mycelial growth of both species of Fusarium. Our results show the great versatility of the mechanisms of action from Trichoderma isolates, mainly associated with the production of volatile organic compounds. Despite the antagonistic effect of Trichoderma isolates observed in vitro, these isolates did not control Fusarium neither promote chickpea growth in in vivo conditions. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2022-05-13T22:19:24Z |
dc.date.available.fl_str_mv |
2022-05-13T22:19:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/41678 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.34117/bjdv6n6-251 |
dc.identifier.issn.pt_BR.fl_str_mv |
2525-8761 |
identifier_str_mv |
10.34117/bjdv6n6-251 2525-8761 |
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http://hdl.handle.net/1843/41678 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of development |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
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Universidade Federal de Minas Gerais |
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Universidade Federal de Minas Gerais (UFMG) |
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UFMG |
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UFMG |
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