A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFMG |
| Texto Completo: | http://hdl.handle.net/1843/41308 |
Resumo: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2022-05-03T01:20:43Z2022-05-03T01:20:43Z2018-0656447948410.1093/mmy/myx0601369-3786http://hdl.handle.net/1843/41308CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorCryptococcus gattii is one of the etiologic agents of cryptococcosis, a systemic mycosis that occurs in healthy and immunosuppressed humans and animals worldwide. Primary pulmonary infection caused by C. gattii is usually followed by fungal dissemination to the central nervous system, resulting in high mortality rates. In this context, animal models of cryptococcosis are useful in the study of fungal pathogenesis and host response against the pathogen, and for testing novel therapeutic options. The most frequently applied method to study fungal dissemination from the lungs to other organs is by culturing tissues, which is not accurate for the detection and quantification of fungal load at early stages of the infection. To overcome this problem, the purpose of this study was to develop a new method for the quantification of Cryptococcus dissemination. One C. gattii strain was efficiently radiolabeled with technetium-99m (99mTc), without affecting viability of the cells. Further, the 99mTc-C. gattii (111 MBq) strain was used to infect mice by intratracheal and intravenous route for biodistribution studies. 99mTc-C. gattii was successfully used in detection of the yeast in the brain of mice 6 hours postinoculation, while the detection using colony forming units was possible only 24 hours postinfection. Our results provided an alternative method that could be applied in further investigations regarding the efficacy of antifungals, fungal virulence, and host-pathogen interactions.engUniversidade Federal de Minas GeraisUFMGBrasilFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASMedical MycologyMicoseCryptococcus gattiiAnimal modelFungal disseminationRadiolabelingCryptococcus gattiiTechnetium-99mA new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattiiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://academic.oup.com/mmy/article-lookup/doi/10.1093/mmy/myx060Marliete Carvalho CostaLays Murta MataNoelly de Queiroz RibeiroAnderson Philip Nonato SantosLorena Vivien Neves OliveiraRaquel Virgínia Rocha VilelaValbert Nascimento CardosoSimone Odília Antunes FernandesDaniel Assis Santosapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/41308/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALA new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii.pdfA new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii.pdfapplication/pdf459068https://repositorio.ufmg.br/bitstream/1843/41308/2/A%20new%20method%20for%20studying%20cryptococcosis%20in%20a%20murine%20model%20using%2099mTc-Cryptococcus%20gattii.pdf6bc2d91399b10237b6aeb6a9df7b4726MD521843/413082022-05-02 22:20:44.478oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-05-03T01:20:44Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
| dc.title.pt_BR.fl_str_mv |
A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii |
| title |
A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii |
| spellingShingle |
A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii Marliete Carvalho Costa Animal model Fungal dissemination Radiolabeling Cryptococcus gattii Technetium-99m Micose Cryptococcus gattii |
| title_short |
A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii |
| title_full |
A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii |
| title_fullStr |
A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii |
| title_full_unstemmed |
A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii |
| title_sort |
A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii |
| author |
Marliete Carvalho Costa |
| author_facet |
Marliete Carvalho Costa Lays Murta Mata Noelly de Queiroz Ribeiro Anderson Philip Nonato Santos Lorena Vivien Neves Oliveira Raquel Virgínia Rocha Vilela Valbert Nascimento Cardoso Simone Odília Antunes Fernandes Daniel Assis Santos |
| author_role |
author |
| author2 |
Lays Murta Mata Noelly de Queiroz Ribeiro Anderson Philip Nonato Santos Lorena Vivien Neves Oliveira Raquel Virgínia Rocha Vilela Valbert Nascimento Cardoso Simone Odília Antunes Fernandes Daniel Assis Santos |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Marliete Carvalho Costa Lays Murta Mata Noelly de Queiroz Ribeiro Anderson Philip Nonato Santos Lorena Vivien Neves Oliveira Raquel Virgínia Rocha Vilela Valbert Nascimento Cardoso Simone Odília Antunes Fernandes Daniel Assis Santos |
| dc.subject.por.fl_str_mv |
Animal model Fungal dissemination Radiolabeling Cryptococcus gattii Technetium-99m |
| topic |
Animal model Fungal dissemination Radiolabeling Cryptococcus gattii Technetium-99m Micose Cryptococcus gattii |
| dc.subject.other.pt_BR.fl_str_mv |
Micose Cryptococcus gattii |
| description |
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2018 |
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2018-06 |
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2022-05-03T01:20:43Z |
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2022-05-03T01:20:43Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://hdl.handle.net/1843/41308 |
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10.1093/mmy/myx060 |
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1369-3786 |
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10.1093/mmy/myx060 1369-3786 |
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Medical Mycology |
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Universidade Federal de Minas Gerais |
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Brasil |
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FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS |
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Universidade Federal de Minas Gerais |
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