Identification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1099/jmm.0.000941 http://hdl.handle.net/1843/46429 |
Resumo: | Purpose: Pythiosis is an infection of humans and other animals caused by the fungal-like pathogen Pythium insidiosum. This pathogen causes life-threatening infection in the infected hosts. Culture, histopathology, serology and molecular tools are used to diagnose its infections. Successful management of pythiosis is directly linked to an early diagnosis. Thus, a rapid identification of putative cultures developing submerged sparsely septate hyphae is of extreme importance. However, few laboratories are familiar with the culture identification of this unique pathogen and its differential diagnosis with similar filamentous fungi. Methodology: We have evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) on 53 isolates of P. insidiosum collected from cases of human and animal pythiosis in the USA and around the world. To assess the specificity of the approach, 18 pathogenic and saprotrophic filamentous fungal and fungal-like microbes were also tested. Results: MALDI-TOF in-house spectra correctly identified the 53 P. insidiosum isolates (score range 1.93–2.51). MALDI-TOF based identification within P. insidiosum isolates showed protein spectra variation between geographical diverse isolates. A mass spectrometry approach was able to discriminate P. insidiosum from the 18 filamentous fungal and fungal-like microbes in this study, including four Pythium spp. and Phytopythium litorale plant pathogenic species. Conclusion: The data showed MALDI-TOF could be used for the accurate and rapid culture identification of P. insidiosum in the clinical laboratory. |
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2022-10-20T11:48:18Z2022-10-20T11:48:18Z2019684574584https://doi.org/10.1099/jmm.0.0009411473-5644http://hdl.handle.net/1843/46429Purpose: Pythiosis is an infection of humans and other animals caused by the fungal-like pathogen Pythium insidiosum. This pathogen causes life-threatening infection in the infected hosts. Culture, histopathology, serology and molecular tools are used to diagnose its infections. Successful management of pythiosis is directly linked to an early diagnosis. Thus, a rapid identification of putative cultures developing submerged sparsely septate hyphae is of extreme importance. However, few laboratories are familiar with the culture identification of this unique pathogen and its differential diagnosis with similar filamentous fungi. Methodology: We have evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) on 53 isolates of P. insidiosum collected from cases of human and animal pythiosis in the USA and around the world. To assess the specificity of the approach, 18 pathogenic and saprotrophic filamentous fungal and fungal-like microbes were also tested. Results: MALDI-TOF in-house spectra correctly identified the 53 P. insidiosum isolates (score range 1.93–2.51). MALDI-TOF based identification within P. insidiosum isolates showed protein spectra variation between geographical diverse isolates. A mass spectrometry approach was able to discriminate P. insidiosum from the 18 filamentous fungal and fungal-like microbes in this study, including four Pythium spp. and Phytopythium litorale plant pathogenic species. Conclusion: The data showed MALDI-TOF could be used for the accurate and rapid culture identification of P. insidiosum in the clinical laboratory.Objetivo: A pitiose é uma infecção de humanos e outros animais causada pelo patógeno fúngico Pythium insidiosum. Este patógeno causa infecção com risco de vida nos hospedeiros infectados. Cultura, histopatologia, sorologia e ferramentas moleculares são utilizadas para diagnosticar suas infecções. O sucesso do manejo da pitiose está diretamente ligado ao diagnóstico precoce. Assim, uma rápida identificação de culturas putativas desenvolvendo hifas escassamente septadas submersas é de extrema importância. No entanto, poucos laboratórios estão familiarizados com a identificação cultural desse patógeno único e seu diagnóstico diferencial com fungos filamentosos semelhantes. Metodologia: Avaliamos o uso da espectrometria de massas com dessorção a laser assistida por matriz-tempo de voo (MALDI-TOF) em 53 isolados de P. insidiosum coletados de casos de pitiose humana e animal nos EUA e no mundo. Para avaliar a especificidade da abordagem, 18 fungos filamentosos patogênicos e saprotróficos e micróbios semelhantes a fungos também foram testados. Resultados: Os espectros internos de MALDI-TOF identificaram corretamente os 53 isolados de P. insidiosum (intervalo de pontuação 1,93–2,51). A identificação baseada em MALDI-TOF em isolados de P. insidiosum mostrou variação nos espectros de proteína entre isolados geográficos diversos. Uma abordagem de espectrometria de massa foi capaz de discriminar P. insidiosum dos 18 fungos filamentosos e micróbios semelhantes a fungos neste estudo, incluindo quatro Pythium spp. e espécies fitopatogênicas de Phytopythium litorale. Conclusão: Os dados mostraram que MALDI-TOF pode ser usado para a identificação precisa e rápida da cultura de P. insidiosum no laboratório clínico.engUniversidade Federal de Minas GeraisUFMGBrasilFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASJournal of Medical MicrobiologyPitioseOomicetosEspectrometria de massas por ionização e dessorção a laser assistida por matrizMALDI-TOFOomycetesOomycotaPythiosisPythium insidiosumIdentification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000941Rinosh ManiRaquel Virgínia Rocha VilelaNiesa KettlerMartin I. ChilversLeonel Mendozaapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/46429/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALIdentification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry.pdfIdentification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry.pdfapplication/pdf1195829https://repositorio.ufmg.br/bitstream/1843/46429/2/Identification%20of%20Pythium%20insidiosum%20complex%20by%20matrix-assisted%20laser%20desorption%20ionization-time%20of%20flight%20mass%20spectrometry.pdf2f24565ddda45f51a53edbf93e5b7754MD521843/464292022-10-20 08:48:18.976oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-10-20T11:48:18Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Identification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry |
title |
Identification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry |
spellingShingle |
Identification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry Rinosh Mani MALDI-TOF Oomycetes Oomycota Pythiosis Pythium insidiosum Pitiose Oomicetos Espectrometria de massas por ionização e dessorção a laser assistida por matriz |
title_short |
Identification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry |
title_full |
Identification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry |
title_fullStr |
Identification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry |
title_full_unstemmed |
Identification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry |
title_sort |
Identification of Pythium insidiosum complex by matrix-assisted laser desorption ionization-time of flight mass spectrometry |
author |
Rinosh Mani |
author_facet |
Rinosh Mani Raquel Virgínia Rocha Vilela Niesa Kettler Martin I. Chilvers Leonel Mendoza |
author_role |
author |
author2 |
Raquel Virgínia Rocha Vilela Niesa Kettler Martin I. Chilvers Leonel Mendoza |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Rinosh Mani Raquel Virgínia Rocha Vilela Niesa Kettler Martin I. Chilvers Leonel Mendoza |
dc.subject.por.fl_str_mv |
MALDI-TOF Oomycetes Oomycota Pythiosis Pythium insidiosum |
topic |
MALDI-TOF Oomycetes Oomycota Pythiosis Pythium insidiosum Pitiose Oomicetos Espectrometria de massas por ionização e dessorção a laser assistida por matriz |
dc.subject.other.pt_BR.fl_str_mv |
Pitiose Oomicetos Espectrometria de massas por ionização e dessorção a laser assistida por matriz |
description |
Purpose: Pythiosis is an infection of humans and other animals caused by the fungal-like pathogen Pythium insidiosum. This pathogen causes life-threatening infection in the infected hosts. Culture, histopathology, serology and molecular tools are used to diagnose its infections. Successful management of pythiosis is directly linked to an early diagnosis. Thus, a rapid identification of putative cultures developing submerged sparsely septate hyphae is of extreme importance. However, few laboratories are familiar with the culture identification of this unique pathogen and its differential diagnosis with similar filamentous fungi. Methodology: We have evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) on 53 isolates of P. insidiosum collected from cases of human and animal pythiosis in the USA and around the world. To assess the specificity of the approach, 18 pathogenic and saprotrophic filamentous fungal and fungal-like microbes were also tested. Results: MALDI-TOF in-house spectra correctly identified the 53 P. insidiosum isolates (score range 1.93–2.51). MALDI-TOF based identification within P. insidiosum isolates showed protein spectra variation between geographical diverse isolates. A mass spectrometry approach was able to discriminate P. insidiosum from the 18 filamentous fungal and fungal-like microbes in this study, including four Pythium spp. and Phytopythium litorale plant pathogenic species. Conclusion: The data showed MALDI-TOF could be used for the accurate and rapid culture identification of P. insidiosum in the clinical laboratory. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2022-10-20T11:48:18Z |
dc.date.available.fl_str_mv |
2022-10-20T11:48:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/46429 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1099/jmm.0.000941 |
dc.identifier.issn.pt_BR.fl_str_mv |
1473-5644 |
url |
https://doi.org/10.1099/jmm.0.000941 http://hdl.handle.net/1843/46429 |
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1473-5644 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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Journal of Medical Microbiology |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Minas Gerais |
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UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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