GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1016/j.mce.2021.111519 http://hdl.handle.net/1843/56253 https://orcid.org/0000-0002-6657-4547 https://orcid.org/0000-0002-0175-642X https://orcid.org/0000-0001-5225-4940 https://orcid.org/0000-0002-2639-3469 https://orcid.org/0000-0003-4577-6243 https://orcid.org/0000-0002-8170-6384 https://orcid.org/0000-0002-8605-8574 https://orcid.org/0000-0002-6185-3491 https://orcid.org/0000-0002-4473-3340 |
Resumo: | GATA-1 is a transcription factor from the GATA family, which features zinc fingers for DNA binding. This protein was initially identified as a crucial regulator of blood cell differentiation, but it is currently known that the Gata-1 gene expression is not limited to this system. Although the testis is also a site of significant GATA-1 expression, its role in testicular cells remains considerably unexplored. In the present study, we evaluated the testicular morphophysiology of adult ΔdblGATA mice with a mutation in the GATA-1 protein. Regarding testicular histology, GATA-1 mutant mice exhibited few changes in the seminiferous tubules, particularly in germ cells. A high proportion of differentiated spermatogonia, an increased number of apoptotic pre-leptotene spermatocytes (Caspase-3-positive), and a high frequency of sperm head defects were observed in ΔdblGATA mice. The main differences were observed in the intertubular compartment, as ΔdblGATA mice showed several morphofunctional changes in Leydig cells. Reduced volume, increased number and down-regulation of steroidogenic enzymes were observed in ΔdblGATA Leydig cells. Moreover, the mutant animal showed lower serum testosterone concentration and high LH levels. These results are consistent with the phenotypic and biometric data of mutant mice, i.e., shorter anogenital index and reduced accessory sexual gland weight. In conclusion, our findings suggest that GATA-1 protein is an important factor for germ cell differentiation as well as for the steroidogenic activity in the testis. |
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2023-07-14T19:30:53Z2023-07-14T19:30:53Z2021542113https://doi.org/10.1016/j.mce.2021.1115190303-7207http://hdl.handle.net/1843/56253https://orcid.org/0000-0002-6657-4547https://orcid.org/0000-0002-0175-642Xhttps://orcid.org/0000-0001-5225-4940https://orcid.org/0000-0002-2639-3469https://orcid.org/0000-0003-4577-6243https://orcid.org/0000-0002-8170-6384https://orcid.org/0000-0002-8605-8574https://orcid.org/0000-0002-6185-3491https://orcid.org/0000-0002-4473-3340GATA-1 is a transcription factor from the GATA family, which features zinc fingers for DNA binding. This protein was initially identified as a crucial regulator of blood cell differentiation, but it is currently known that the Gata-1 gene expression is not limited to this system. Although the testis is also a site of significant GATA-1 expression, its role in testicular cells remains considerably unexplored. In the present study, we evaluated the testicular morphophysiology of adult ΔdblGATA mice with a mutation in the GATA-1 protein. Regarding testicular histology, GATA-1 mutant mice exhibited few changes in the seminiferous tubules, particularly in germ cells. A high proportion of differentiated spermatogonia, an increased number of apoptotic pre-leptotene spermatocytes (Caspase-3-positive), and a high frequency of sperm head defects were observed in ΔdblGATA mice. The main differences were observed in the intertubular compartment, as ΔdblGATA mice showed several morphofunctional changes in Leydig cells. Reduced volume, increased number and down-regulation of steroidogenic enzymes were observed in ΔdblGATA Leydig cells. Moreover, the mutant animal showed lower serum testosterone concentration and high LH levels. These results are consistent with the phenotypic and biometric data of mutant mice, i.e., shorter anogenital index and reduced accessory sexual gland weight. In conclusion, our findings suggest that GATA-1 protein is an important factor for germ cell differentiation as well as for the steroidogenic activity in the testis.porUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE MORFOLOGIAMolecular and Cellular EndocrinologyMorfologiaLeydig, Celulas deTesticulosTestosteronaLeydig cellTestisΔdblGATATestosteroneGerm cell differentiationGATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.sciencedirect.com/science/article/pii/S0303720721003634?via%3DihubMatheus Felipe Fonseca GonçalvesVivian Vasconcelos CostaAna Clara Campideli SantanaRoberta Araújo LopesRaphael Escorsim SzawkaGuilherme Mattos Jardim CostaSamyra Maria dos Santos Nassif LacerdaNathália de Lima e Martins LaraCarolina Felipe Alves de OliveiraAndré Felipe de Figueiredo AlmeidaMarcos Rocha Gouvêa BrenerMarina Alcântara CavalcanteAnderson Kenedy SantosGabriel Henrique Campolina Silvainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/56253/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALGATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis.pdfGATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis.pdfapplication/pdf55455938https://repositorio.ufmg.br/bitstream/1843/56253/2/GATA-1%20mutation%20alters%20the%20spermatogonial%20phase%20and%20steroidogenesis%20in%20adult%20mouse%20testis.pdf9039159e646ff9cc91c6b1a8db4367afMD521843/562532023-07-14 16:30:53.706oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-07-14T19:30:53Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis |
title |
GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis |
spellingShingle |
GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis Matheus Felipe Fonseca Gonçalves Leydig cell Testis ΔdblGATA Testosterone Germ cell differentiation Morfologia Leydig, Celulas de Testiculos Testosterona |
title_short |
GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis |
title_full |
GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis |
title_fullStr |
GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis |
title_full_unstemmed |
GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis |
title_sort |
GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis |
author |
Matheus Felipe Fonseca Gonçalves |
author_facet |
Matheus Felipe Fonseca Gonçalves Vivian Vasconcelos Costa Ana Clara Campideli Santana Roberta Araújo Lopes Raphael Escorsim Szawka Guilherme Mattos Jardim Costa Samyra Maria dos Santos Nassif Lacerda Nathália de Lima e Martins Lara Carolina Felipe Alves de Oliveira André Felipe de Figueiredo Almeida Marcos Rocha Gouvêa Brener Marina Alcântara Cavalcante Anderson Kenedy Santos Gabriel Henrique Campolina Silva |
author_role |
author |
author2 |
Vivian Vasconcelos Costa Ana Clara Campideli Santana Roberta Araújo Lopes Raphael Escorsim Szawka Guilherme Mattos Jardim Costa Samyra Maria dos Santos Nassif Lacerda Nathália de Lima e Martins Lara Carolina Felipe Alves de Oliveira André Felipe de Figueiredo Almeida Marcos Rocha Gouvêa Brener Marina Alcântara Cavalcante Anderson Kenedy Santos Gabriel Henrique Campolina Silva |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Matheus Felipe Fonseca Gonçalves Vivian Vasconcelos Costa Ana Clara Campideli Santana Roberta Araújo Lopes Raphael Escorsim Szawka Guilherme Mattos Jardim Costa Samyra Maria dos Santos Nassif Lacerda Nathália de Lima e Martins Lara Carolina Felipe Alves de Oliveira André Felipe de Figueiredo Almeida Marcos Rocha Gouvêa Brener Marina Alcântara Cavalcante Anderson Kenedy Santos Gabriel Henrique Campolina Silva |
dc.subject.por.fl_str_mv |
Leydig cell Testis ΔdblGATA Testosterone Germ cell differentiation |
topic |
Leydig cell Testis ΔdblGATA Testosterone Germ cell differentiation Morfologia Leydig, Celulas de Testiculos Testosterona |
dc.subject.other.pt_BR.fl_str_mv |
Morfologia Leydig, Celulas de Testiculos Testosterona |
description |
GATA-1 is a transcription factor from the GATA family, which features zinc fingers for DNA binding. This protein was initially identified as a crucial regulator of blood cell differentiation, but it is currently known that the Gata-1 gene expression is not limited to this system. Although the testis is also a site of significant GATA-1 expression, its role in testicular cells remains considerably unexplored. In the present study, we evaluated the testicular morphophysiology of adult ΔdblGATA mice with a mutation in the GATA-1 protein. Regarding testicular histology, GATA-1 mutant mice exhibited few changes in the seminiferous tubules, particularly in germ cells. A high proportion of differentiated spermatogonia, an increased number of apoptotic pre-leptotene spermatocytes (Caspase-3-positive), and a high frequency of sperm head defects were observed in ΔdblGATA mice. The main differences were observed in the intertubular compartment, as ΔdblGATA mice showed several morphofunctional changes in Leydig cells. Reduced volume, increased number and down-regulation of steroidogenic enzymes were observed in ΔdblGATA Leydig cells. Moreover, the mutant animal showed lower serum testosterone concentration and high LH levels. These results are consistent with the phenotypic and biometric data of mutant mice, i.e., shorter anogenital index and reduced accessory sexual gland weight. In conclusion, our findings suggest that GATA-1 protein is an important factor for germ cell differentiation as well as for the steroidogenic activity in the testis. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021 |
dc.date.accessioned.fl_str_mv |
2023-07-14T19:30:53Z |
dc.date.available.fl_str_mv |
2023-07-14T19:30:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/56253 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1016/j.mce.2021.111519 |
dc.identifier.issn.pt_BR.fl_str_mv |
0303-7207 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-6657-4547 https://orcid.org/0000-0002-0175-642X https://orcid.org/0000-0001-5225-4940 https://orcid.org/0000-0002-2639-3469 https://orcid.org/0000-0003-4577-6243 https://orcid.org/0000-0002-8170-6384 https://orcid.org/0000-0002-8605-8574 https://orcid.org/0000-0002-6185-3491 https://orcid.org/0000-0002-4473-3340 |
url |
https://doi.org/10.1016/j.mce.2021.111519 http://hdl.handle.net/1843/56253 https://orcid.org/0000-0002-6657-4547 https://orcid.org/0000-0002-0175-642X https://orcid.org/0000-0001-5225-4940 https://orcid.org/0000-0002-2639-3469 https://orcid.org/0000-0003-4577-6243 https://orcid.org/0000-0002-8170-6384 https://orcid.org/0000-0002-8605-8574 https://orcid.org/0000-0002-6185-3491 https://orcid.org/0000-0002-4473-3340 |
identifier_str_mv |
0303-7207 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Molecular and Cellular Endocrinology |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICB - DEPARTAMENTO DE MORFOLOGIA |
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Universidade Federal de Minas Gerais |
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