EFEITO DE UMA AZIDA ORGÂNICA SOBRE A ATIVIDADE DA NTPDase EM LINFÓCITOS HUMANOS

• Autor(a) principal: Lopes, Luciana dos Santos
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional Universidade Franciscana
• Texto Completo: http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/237; http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/344
• topic: NTPDase; Inibidores enzimáticos; Azidas orgânicas; NTPDase; Enzyme inhibitors; Organic azides; CNPQ::ENGENHARIAS

The NTPDase (apirase-ect, ect-difosfoidrolase, CD39, EC 3.6.1.5) hydrolyses tri nucleotides and/or diphosphate. It is an ectonucleotidase, identified as the antigen surface of lymphoid cells, whose greatest expression leads to an increase in activity of ATPase and ADPase and these cells through the hydrolysis of ATP and ADP. The inhibitor of ecto-nucleotidases may represent valuable therapeutic tools to amplify the biological effects of extracellular nucleotides, since they induce apoptosis in lymphocytes, causing a state of immunosuppression. It is known through the literature that sodium azide produces a significant inhibition in the hydrolysis of ATP and ADP, caused this enzyme when it is used in concentrations around 20mM. The azides are chemical components widely used in organic synthesis, and remarkably stable in biological environment. The aim of this study was to determine the inhibitory effects of an organic azide derived from acetic acid in the hydrolysis of ATP and ADP, which is the activity of NTPDase of human lymphocytes. Since changes in distribution and activity of this enzyme have been reported in various pathological conditions, demonstrating its participation in the activation of lymphocyte, it is believed that the identification of new compounds inhibiting NTPDase will contribute to treatment of diseases where there is a need for immunosuppression, as allergies and autoimmune diseases. After incubation of lymphocytes in medium containing different concentrations of azide organic, there was a significant inhibition of ADP in the hydrolysis when used concentrations of 10 and 20 mM, and this of 50 and 77% respectively. Unverified inhibitory effects on the ATP hydrolysis with organic azide. A changed the hydrolysis of nucleotide adenine diphosphate, indicating that the substance could be used as an inhibitor of mixed NTPDase.