DETERMINAÇÃO DA ATIVIDADE BIOLÓGICA DE ÓXIDO DE GRAFENO (GO) DECORADO COM FERRITA (Fe3O4)

• Autor(a) principal: Oliveira, Évelin Cogo de
• Data de Publicação: 2020
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional Universidade Franciscana
• Texto Completo: http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/900
• topic: nanotoxicologia; ecotoxicologia; nanopartículas magnéticas; microcrustáceos; atividade antimicrobiana.; nanotoxicology; ecotoxicology; magnetic nanoparticles; microcrustaceans; antimicrobian activity.; ENGENHARIA/TECNOLOGIA/GESTÃO

Studies carried out by the UN have shown that in the year 2050 bacterial resistance will be so dangerous that it could kill more than 10 million people, causing more deaths than cancer. Given this fact, we sought to conduct a study to minimize this problem using graphene oxide (GO), which was obtained through the exfoliation of graphite. Graphene oxide has attracted a lot of attention, as it is a highly versatile structure, which allowed it to be used as a carrier for magnetic nanoparticles, making magnetic GO effective as an antimicrobial agent, as demonstrated in the study. To carry out its incorporation, a technique of adding iron nanoparticles to the GO was used, which does not use agents that are aggressive to the environment. Corroborating with the concepts applied in green chemistry, since pollution and damage to the environment are increasing due to the incorrect disposal of antimicrobials. After the synthesis and characterization studies, each of the nanostructured systems produced was analyzed in relation to the following activities: cell cytotoxicity by the methods of DNAPicoGreen®, dichlorofluorescein diacetate (DCFH-DA), determination of nitric oxide, cell viability by the MTT assay , antimicrobial activity by disk-diffusion, antimicrobial activity by microdilution in broth and ecotoxic activity by bioassay with Artemia salina. In the tests carried out, the nanostructure with a lower amount of Fe3O4 showed significant antimicrobial activity against the strains of S. aureus and E. faecium, being important not only because of its activity, but because this nanostructure can be rescued from the environment through the use of a magnetic field, decreasing the amount of antimicrobials in the environment and contributing to the decrease of bacterial resistance. The results in relation to toxicity, showed low cyto activity and ecotoxicity of the nanostructured systems tested with their respective controls, presenting safety in the lowest concentrations. With these results we were able to produce a nanocomposite capable of being rescued from the environment, presenting antimicrobial activity, having no acute toxicity in Artemia salina and not showing cellular toxicity in the lowest concentrations