Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/8135 http://dx.doi.org/10.1155/2012/962538 |
Resumo: | The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-termstrategy to control schistosomiasis is through immunization with an antischistosomiasis vaccine combined with drug treatment. In the search for potential vaccine candidates, numerous tegument antigens have been assessed. As the major interface between parasite and mammalian host, the tegument plays crucial roles in the establishment and further course of schistosomiasis. Herein, we evaluated the potential of a GPI fraction, containing representative molecules located on the outer surface of adult worms, as vaccine candidate. Immunization of mice with GPI-anchored proteins induced a mixed Th1/Th2 type of immune response with production of IFN-γ and TNF-α, and low levels of IL-5 into the supernatant of splenocyte cultures. The protection engendered by this vaccination protocol was confirmed by 42% reduction in worm burden, 45% reduction in eggs per gram of hepatic tissue, 29% reduction in the number of granulomas per area, and 53% reduction in the granuloma fibrosis. Taken together, the data herein support the potential of surface-exposed GPI-anchored antigens from the S. mansoni tegument as vaccine candidate. |
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Martins, Vicente de PauloPinheiro, Carina da SilvaFigueiredo, Bárbara de Castro PimentelAssis, Natan Raimundo Gonçalves deMorais, Suellen Batistoni deCaliari, Marcelo VidigalAzevedo, VascoBorges, William de CastroWilson, R. AlanOliveira, Sergio Costa2017-07-03T17:54:48Z2017-07-03T17:54:48Z2012MARTINS, V. de P. et al. Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection. Clinical and Developmental Immunology, v. 2012, p. e962538, 2012. Disponível em: <https://www.hindawi.com/journals/jir/2012/962538/>. Acesso em: 20 mar. 2017.2314-7156http://www.repositorio.ufop.br/handle/123456789/8135http://dx.doi.org/10.1155/2012/962538The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-termstrategy to control schistosomiasis is through immunization with an antischistosomiasis vaccine combined with drug treatment. In the search for potential vaccine candidates, numerous tegument antigens have been assessed. As the major interface between parasite and mammalian host, the tegument plays crucial roles in the establishment and further course of schistosomiasis. Herein, we evaluated the potential of a GPI fraction, containing representative molecules located on the outer surface of adult worms, as vaccine candidate. Immunization of mice with GPI-anchored proteins induced a mixed Th1/Th2 type of immune response with production of IFN-γ and TNF-α, and low levels of IL-5 into the supernatant of splenocyte cultures. The protection engendered by this vaccination protocol was confirmed by 42% reduction in worm burden, 45% reduction in eggs per gram of hepatic tissue, 29% reduction in the number of granulomas per area, and 53% reduction in the granuloma fibrosis. Taken together, the data herein support the potential of surface-exposed GPI-anchored antigens from the S. mansoni tegument as vaccine candidate.This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: o próprio artigo.info:eu-repo/semantics/openAccessVaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/8135/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_VaccinationEnzynaticallyCleaved.pdfARTIGO_VaccinationEnzynaticallyCleaved.pdfapplication/pdf4624144http://www.repositorio.ufop.br/bitstream/123456789/8135/1/ARTIGO_VaccinationEnzynaticallyCleaved.pdf51680c943b6e9fdfe736ba510573028fMD51123456789/81352019-12-10 11:45:03.462oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-12-10T16:45:03Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection. |
title |
Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection. |
spellingShingle |
Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection. Martins, Vicente de Paulo |
title_short |
Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection. |
title_full |
Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection. |
title_fullStr |
Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection. |
title_full_unstemmed |
Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection. |
title_sort |
Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection. |
author |
Martins, Vicente de Paulo |
author_facet |
Martins, Vicente de Paulo Pinheiro, Carina da Silva Figueiredo, Bárbara de Castro Pimentel Assis, Natan Raimundo Gonçalves de Morais, Suellen Batistoni de Caliari, Marcelo Vidigal Azevedo, Vasco Borges, William de Castro Wilson, R. Alan Oliveira, Sergio Costa |
author_role |
author |
author2 |
Pinheiro, Carina da Silva Figueiredo, Bárbara de Castro Pimentel Assis, Natan Raimundo Gonçalves de Morais, Suellen Batistoni de Caliari, Marcelo Vidigal Azevedo, Vasco Borges, William de Castro Wilson, R. Alan Oliveira, Sergio Costa |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Martins, Vicente de Paulo Pinheiro, Carina da Silva Figueiredo, Bárbara de Castro Pimentel Assis, Natan Raimundo Gonçalves de Morais, Suellen Batistoni de Caliari, Marcelo Vidigal Azevedo, Vasco Borges, William de Castro Wilson, R. Alan Oliveira, Sergio Costa |
description |
The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-termstrategy to control schistosomiasis is through immunization with an antischistosomiasis vaccine combined with drug treatment. In the search for potential vaccine candidates, numerous tegument antigens have been assessed. As the major interface between parasite and mammalian host, the tegument plays crucial roles in the establishment and further course of schistosomiasis. Herein, we evaluated the potential of a GPI fraction, containing representative molecules located on the outer surface of adult worms, as vaccine candidate. Immunization of mice with GPI-anchored proteins induced a mixed Th1/Th2 type of immune response with production of IFN-γ and TNF-α, and low levels of IL-5 into the supernatant of splenocyte cultures. The protection engendered by this vaccination protocol was confirmed by 42% reduction in worm burden, 45% reduction in eggs per gram of hepatic tissue, 29% reduction in the number of granulomas per area, and 53% reduction in the granuloma fibrosis. Taken together, the data herein support the potential of surface-exposed GPI-anchored antigens from the S. mansoni tegument as vaccine candidate. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2017-07-03T17:54:48Z |
dc.date.available.fl_str_mv |
2017-07-03T17:54:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARTINS, V. de P. et al. Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection. Clinical and Developmental Immunology, v. 2012, p. e962538, 2012. Disponível em: <https://www.hindawi.com/journals/jir/2012/962538/>. Acesso em: 20 mar. 2017. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/8135 |
dc.identifier.issn.none.fl_str_mv |
2314-7156 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1155/2012/962538 |
identifier_str_mv |
MARTINS, V. de P. et al. Vaccination with enzimatically cleaved GPI-anchored proteins from Schistosoma mansoni induced protection against challenge infection. Clinical and Developmental Immunology, v. 2012, p. e962538, 2012. Disponível em: <https://www.hindawi.com/journals/jir/2012/962538/>. Acesso em: 20 mar. 2017. 2314-7156 |
url |
http://www.repositorio.ufop.br/handle/123456789/8135 http://dx.doi.org/10.1155/2012/962538 |
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eng |
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