Relationship of Leishmania-specific IgG levels and IgG avidity with parasite density and clinical signs in canine leishmaniasis.

• Autor(a) principal: Teixeira Neto, Rafael Gonçalves
• Data de Publicação: 2010
• Outros Autores: Giunchetti, Rodolfo Cordeiro, Carneiro, Cláudia Martins, Vitor, Ricardo Wagner de Almeida, Vital, Wendel Coura, Quaresma, Patrícia Flávia, Ker, Henrique Gama, Melo, Lutiana Amaral de, Gontijo, Célia Maria Ferreira, Reis, Alexandre Barbosa
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFOP
• Texto Completo: http://www.repositorio.ufop.br/handle/123456789/959
• topic: Canine visceral leishmaniasis; Parasite burden; Immunoglobulin avidity

The clinical status and tissue parasite burden of the skin and spleen of 40 dogs naturally infected with Leishmania chagasi (syn. Leishmania infantum), together with 5 uninfected control dogs, were assessed. On the basis of the clinical evaluation, infected dogs were classified as asymptomatic (AD) or symptomatic (SD). Infected animals were also grouped according to their parasite load as exhibiting low (LP), medium (MP) and high (HP) parasitism. The results indicated a high parasite load in the skin samples of SD animals in relation to the AD group. The serum immunoglobin isotype profiles of the studied animals revealed increased levels of IgG 1 in the AD and LP dogs, whereas high levels of IgG 2 were correlated with SD and HP dogs. The avidity index (AI) of IgG total in the SD group was high in comparison of that of the AD group. Moreover, animals with a larger parasite burden either in the spleen or skin showed higher AI values than animals with lower parasitism. Based on these findings, it is suggested that CVL commences with an asymptomatic clinical form with low parasitism, high production of IgG 1 and low affinity of IgG total molecules, and evolves into a symptomatic clinical form with higher parasitism intensity, higher IgG 2 levels, and high affinity of IgG total