Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats.
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Publication Date: | 2006 |
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Format: | Article |
Language: | eng |
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Download full: | http://www.repositorio.ufop.br/handle/123456789/4668 https://doi.org/10.1016/j.brainresbull.2006.07.013 |
Summary: | Reactive oxygen species (ROS) have been shown to modulate neuronal synaptic transmission and may play a role on the autonomic control of the cardiovascular system. In this study we investigated the effects produced by hydrogen peroxide (H2O2) injected alone or combined with the anti-oxidant agent N-acetil-l-cysteine (NAC) or catalase into the fourth brain ventricle (4th V) on mean arterial pressure and heart rate of conscious rats. Moreover the involvement of the autonomic nervous system on the cardiovascular responses to H2O2 into the 4th V was also investigated. Male Holtzman rats (280–320 g) with a stainless steel cannula implanted into the 4th V and polyethylene cannulas inserted into the femoral artery and vein were used. Injections of H2O2 (0.5, 1.0 and 1.5 _mol/0.2 _L, n = 6) into the 4th V produced transient (for 10 min) dose-dependent pressor responses. The 1.0 and 1.5 _mol doses of H2O2 also produced a long lasting bradycardia (at least 24 h with the high dose of H2O2). Prior injection of N-acetyl-l-cysteine (250 nmol/1 _L/rat) into the 4th V blockade the pressor response and attenuated the bradycardic response to H2O2 (1_mol/0.5 _L/rat, n = 7) into the 4th V. Intravenous (i.v.) atropine methyl bromide (1.0 mg/kg, n = 11) abolished the bradycardia but did not affect the pressor response to H2O2. Prazosin hydrochloride (1.0 mg/kg, n=6) i.v. abolished the pressor response but did not affect the bradycardia. The increase in the catalase activity (500 UEA/1 _L/rat injected into the 4th V) also abolished both, pressor and bradycardic responses to H2O2. The results suggest that increased ROS availability into 4th V simultaneously activate sympathetic and parasympathetic outflow inducing pressor and bradycardic responses. |
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Cardoso, Leonardo MáximoColombari, Débora Simões de AlmeidaMenani, José VanderleiChianca Júnior, Deoclécio AlvesColombari, Eduardo2015-03-16T18:59:47Z2015-03-16T18:59:47Z2006CARDOSO, L. M. et al. Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats. Brain Research Bulletin , v. 71, p. 37-44, 2006. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0361923006002413>. Acesso em: 08 nov. 2014.0361-9230http://www.repositorio.ufop.br/handle/123456789/4668https://doi.org/10.1016/j.brainresbull.2006.07.013Reactive oxygen species (ROS) have been shown to modulate neuronal synaptic transmission and may play a role on the autonomic control of the cardiovascular system. In this study we investigated the effects produced by hydrogen peroxide (H2O2) injected alone or combined with the anti-oxidant agent N-acetil-l-cysteine (NAC) or catalase into the fourth brain ventricle (4th V) on mean arterial pressure and heart rate of conscious rats. Moreover the involvement of the autonomic nervous system on the cardiovascular responses to H2O2 into the 4th V was also investigated. Male Holtzman rats (280–320 g) with a stainless steel cannula implanted into the 4th V and polyethylene cannulas inserted into the femoral artery and vein were used. Injections of H2O2 (0.5, 1.0 and 1.5 _mol/0.2 _L, n = 6) into the 4th V produced transient (for 10 min) dose-dependent pressor responses. The 1.0 and 1.5 _mol doses of H2O2 also produced a long lasting bradycardia (at least 24 h with the high dose of H2O2). Prior injection of N-acetyl-l-cysteine (250 nmol/1 _L/rat) into the 4th V blockade the pressor response and attenuated the bradycardic response to H2O2 (1_mol/0.5 _L/rat, n = 7) into the 4th V. Intravenous (i.v.) atropine methyl bromide (1.0 mg/kg, n = 11) abolished the bradycardia but did not affect the pressor response to H2O2. Prazosin hydrochloride (1.0 mg/kg, n=6) i.v. abolished the pressor response but did not affect the bradycardia. The increase in the catalase activity (500 UEA/1 _L/rat injected into the 4th V) also abolished both, pressor and bradycardic responses to H2O2. The results suggest that increased ROS availability into 4th V simultaneously activate sympathetic and parasympathetic outflow inducing pressor and bradycardic responses.Hydrogen peroxideBlood pressureHeart ratePrazosinMethyl-atropineCardiovascular responses produced by central injection of hydrogen peroxide in conscious rats.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Brain Research Bulletin concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3520371395113.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-82636http://www.repositorio.ufop.br/bitstream/123456789/4668/2/license.txtc2ffdd99e58acf69202dff00d361f23aMD52ORIGINALARTIGO_CardiovascularresponsesProduced.pdfARTIGO_CardiovascularresponsesProduced.pdfapplication/pdf487956http://www.repositorio.ufop.br/bitstream/123456789/4668/1/ARTIGO_CardiovascularresponsesProduced.pdf1071fe25299b8f28b91a645b46ba441cMD51123456789/46682019-06-25 13:52:49.62oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-06-25T17:52:49Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats. |
title |
Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats. |
spellingShingle |
Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats. Cardoso, Leonardo Máximo Hydrogen peroxide Blood pressure Heart rate Prazosin Methyl-atropine |
title_short |
Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats. |
title_full |
Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats. |
title_fullStr |
Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats. |
title_full_unstemmed |
Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats. |
title_sort |
Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats. |
author |
Cardoso, Leonardo Máximo |
author_facet |
Cardoso, Leonardo Máximo Colombari, Débora Simões de Almeida Menani, José Vanderlei Chianca Júnior, Deoclécio Alves Colombari, Eduardo |
author_role |
author |
author2 |
Colombari, Débora Simões de Almeida Menani, José Vanderlei Chianca Júnior, Deoclécio Alves Colombari, Eduardo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Cardoso, Leonardo Máximo Colombari, Débora Simões de Almeida Menani, José Vanderlei Chianca Júnior, Deoclécio Alves Colombari, Eduardo |
dc.subject.por.fl_str_mv |
Hydrogen peroxide Blood pressure Heart rate Prazosin Methyl-atropine |
topic |
Hydrogen peroxide Blood pressure Heart rate Prazosin Methyl-atropine |
description |
Reactive oxygen species (ROS) have been shown to modulate neuronal synaptic transmission and may play a role on the autonomic control of the cardiovascular system. In this study we investigated the effects produced by hydrogen peroxide (H2O2) injected alone or combined with the anti-oxidant agent N-acetil-l-cysteine (NAC) or catalase into the fourth brain ventricle (4th V) on mean arterial pressure and heart rate of conscious rats. Moreover the involvement of the autonomic nervous system on the cardiovascular responses to H2O2 into the 4th V was also investigated. Male Holtzman rats (280–320 g) with a stainless steel cannula implanted into the 4th V and polyethylene cannulas inserted into the femoral artery and vein were used. Injections of H2O2 (0.5, 1.0 and 1.5 _mol/0.2 _L, n = 6) into the 4th V produced transient (for 10 min) dose-dependent pressor responses. The 1.0 and 1.5 _mol doses of H2O2 also produced a long lasting bradycardia (at least 24 h with the high dose of H2O2). Prior injection of N-acetyl-l-cysteine (250 nmol/1 _L/rat) into the 4th V blockade the pressor response and attenuated the bradycardic response to H2O2 (1_mol/0.5 _L/rat, n = 7) into the 4th V. Intravenous (i.v.) atropine methyl bromide (1.0 mg/kg, n = 11) abolished the bradycardia but did not affect the pressor response to H2O2. Prazosin hydrochloride (1.0 mg/kg, n=6) i.v. abolished the pressor response but did not affect the bradycardia. The increase in the catalase activity (500 UEA/1 _L/rat injected into the 4th V) also abolished both, pressor and bradycardic responses to H2O2. The results suggest that increased ROS availability into 4th V simultaneously activate sympathetic and parasympathetic outflow inducing pressor and bradycardic responses. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006 |
dc.date.accessioned.fl_str_mv |
2015-03-16T18:59:47Z |
dc.date.available.fl_str_mv |
2015-03-16T18:59:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CARDOSO, L. M. et al. Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats. Brain Research Bulletin , v. 71, p. 37-44, 2006. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0361923006002413>. Acesso em: 08 nov. 2014. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/4668 |
dc.identifier.issn.none.fl_str_mv |
0361-9230 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.brainresbull.2006.07.013 |
identifier_str_mv |
CARDOSO, L. M. et al. Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats. Brain Research Bulletin , v. 71, p. 37-44, 2006. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0361923006002413>. Acesso em: 08 nov. 2014. 0361-9230 |
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http://www.repositorio.ufop.br/handle/123456789/4668 https://doi.org/10.1016/j.brainresbull.2006.07.013 |
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