Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2002 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/1417 |
Resumo: | In this study, the polymerase chain reaction (PCR) was compared with parasitological and serological methods to detect the infection in dogs, 5–12 years after experimental infection with Trypanosoma cruzi. The ability of parasitological methods to identify a positive animal was 22 and 11% by hemoculture and xenodiagnosis/xenoculture, respectively. On the other hand, the serological tests, including conventional serology and anti-live trypomastigote antibodies (ALTA) were positive in all infected dogs. Despite its low sensitivity, if considering only one reaction, the PCR analysis showed 100% of positivity, demonstrating the presence of parasite kDNA in all infected dogs. To identify a positive dog required at least two blood samples and up to nine repeated reactions using the same sample. Serial blood sample collection, ranging from 1 to 9, revealed that the percentage of dogs with positive PCR ranged from 67 to 100%. These findings suggested that, although the PCR is useful to detect the parasite in infected hosts, it should not be used isolated for the diagnosis of Chagas’ disease and warn for the necessity of serial blood collection and re-tests. Moreover, these data validate once more the dog as a model for Chagas’ disease since they demonstrate the permanence of infection by PCR, parasitological and serological methods, reaching relevant requisites for an ideal model to study this disease. |
id |
UFOP_87e38b5cfc0b05f5ecb9309e3f247dfa |
---|---|
oai_identifier_str |
oai:localhost:123456789/1417 |
network_acronym_str |
UFOP |
network_name_str |
Repositório Institucional da UFOP |
repository_id_str |
3233 |
spelling |
Araújo, Flávio Marcos Gomes deBahia, Maria TerezinhaMagalhães, Neuza Maria deMartins Filho, Olindo AssisVeloso, Vanja MariaCarneiro, Cláudia MartinsTafuri, Washington LuizLana, Marta de2012-09-25T13:06:17Z2012-09-25T13:06:17Z2002ARAÚJO, F. M. G. de et al. Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods. Acta Tropica, v. 81, n. 1, p. 21-31, jan. 2002. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0001706X01001966>. Acesso em: 25 set. 2012.0001706Xhttp://www.repositorio.ufop.br/handle/123456789/1417In this study, the polymerase chain reaction (PCR) was compared with parasitological and serological methods to detect the infection in dogs, 5–12 years after experimental infection with Trypanosoma cruzi. The ability of parasitological methods to identify a positive animal was 22 and 11% by hemoculture and xenodiagnosis/xenoculture, respectively. On the other hand, the serological tests, including conventional serology and anti-live trypomastigote antibodies (ALTA) were positive in all infected dogs. Despite its low sensitivity, if considering only one reaction, the PCR analysis showed 100% of positivity, demonstrating the presence of parasite kDNA in all infected dogs. To identify a positive dog required at least two blood samples and up to nine repeated reactions using the same sample. Serial blood sample collection, ranging from 1 to 9, revealed that the percentage of dogs with positive PCR ranged from 67 to 100%. These findings suggested that, although the PCR is useful to detect the parasite in infected hosts, it should not be used isolated for the diagnosis of Chagas’ disease and warn for the necessity of serial blood collection and re-tests. Moreover, these data validate once more the dog as a model for Chagas’ disease since they demonstrate the permanence of infection by PCR, parasitological and serological methods, reaching relevant requisites for an ideal model to study this disease.Chronic infectionParasitological tests and serologyTrypanosoma cruziDogFollow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Acta Tropica concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3282590943953.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/1417/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55ORIGINALARTIGO_FollowUpExperimental.pdfARTIGO_FollowUpExperimental.pdfapplication/pdf335729http://www.repositorio.ufop.br/bitstream/123456789/1417/1/ARTIGO_FollowUpExperimental.pdfc69f70b51c5c496958eb75e1fe4c8bcdMD51123456789/14172019-03-08 11:47:24.657oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-03-08T16:47:24Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods. |
title |
Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods. |
spellingShingle |
Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods. Araújo, Flávio Marcos Gomes de Chronic infection Parasitological tests and serology Trypanosoma cruzi Dog |
title_short |
Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods. |
title_full |
Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods. |
title_fullStr |
Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods. |
title_full_unstemmed |
Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods. |
title_sort |
Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods. |
author |
Araújo, Flávio Marcos Gomes de |
author_facet |
Araújo, Flávio Marcos Gomes de Bahia, Maria Terezinha Magalhães, Neuza Maria de Martins Filho, Olindo Assis Veloso, Vanja Maria Carneiro, Cláudia Martins Tafuri, Washington Luiz Lana, Marta de |
author_role |
author |
author2 |
Bahia, Maria Terezinha Magalhães, Neuza Maria de Martins Filho, Olindo Assis Veloso, Vanja Maria Carneiro, Cláudia Martins Tafuri, Washington Luiz Lana, Marta de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Araújo, Flávio Marcos Gomes de Bahia, Maria Terezinha Magalhães, Neuza Maria de Martins Filho, Olindo Assis Veloso, Vanja Maria Carneiro, Cláudia Martins Tafuri, Washington Luiz Lana, Marta de |
dc.subject.por.fl_str_mv |
Chronic infection Parasitological tests and serology Trypanosoma cruzi Dog |
topic |
Chronic infection Parasitological tests and serology Trypanosoma cruzi Dog |
description |
In this study, the polymerase chain reaction (PCR) was compared with parasitological and serological methods to detect the infection in dogs, 5–12 years after experimental infection with Trypanosoma cruzi. The ability of parasitological methods to identify a positive animal was 22 and 11% by hemoculture and xenodiagnosis/xenoculture, respectively. On the other hand, the serological tests, including conventional serology and anti-live trypomastigote antibodies (ALTA) were positive in all infected dogs. Despite its low sensitivity, if considering only one reaction, the PCR analysis showed 100% of positivity, demonstrating the presence of parasite kDNA in all infected dogs. To identify a positive dog required at least two blood samples and up to nine repeated reactions using the same sample. Serial blood sample collection, ranging from 1 to 9, revealed that the percentage of dogs with positive PCR ranged from 67 to 100%. These findings suggested that, although the PCR is useful to detect the parasite in infected hosts, it should not be used isolated for the diagnosis of Chagas’ disease and warn for the necessity of serial blood collection and re-tests. Moreover, these data validate once more the dog as a model for Chagas’ disease since they demonstrate the permanence of infection by PCR, parasitological and serological methods, reaching relevant requisites for an ideal model to study this disease. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002 |
dc.date.accessioned.fl_str_mv |
2012-09-25T13:06:17Z |
dc.date.available.fl_str_mv |
2012-09-25T13:06:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARAÚJO, F. M. G. de et al. Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods. Acta Tropica, v. 81, n. 1, p. 21-31, jan. 2002. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0001706X01001966>. Acesso em: 25 set. 2012. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/1417 |
dc.identifier.issn.none.fl_str_mv |
0001706X |
identifier_str_mv |
ARAÚJO, F. M. G. de et al. Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods. Acta Tropica, v. 81, n. 1, p. 21-31, jan. 2002. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0001706X01001966>. Acesso em: 25 set. 2012. 0001706X |
url |
http://www.repositorio.ufop.br/handle/123456789/1417 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
bitstream.url.fl_str_mv |
http://www.repositorio.ufop.br/bitstream/123456789/1417/5/license.txt http://www.repositorio.ufop.br/bitstream/123456789/1417/1/ARTIGO_FollowUpExperimental.pdf |
bitstream.checksum.fl_str_mv |
8a4605be74aa9ea9d79846c1fba20a33 c69f70b51c5c496958eb75e1fe4c8bcd |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
_version_ |
1797950113584250880 |