CD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice.
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/4629 https://doi.org/10.1016/j.micinf.2007.05.016 |
Resumo: | Vaccine-induced protection against leishmaniasis is largely dependent on cell-mediated type 1 response and IL-12-driven IFN-g production. Surprisingly, our previous data showed that IL-12/23p40_/_ mice could be vaccinated against L. amazonensis and were able to produce limited amounts of IFN-g. Since the role of CD8þT in immunization against L. amazonensis is obscure, the aim of this study was to evaluate the effects of CD8þ cells in protection against L. amazonensis in IL-12/23p40_/_ mice. In order to deplete CD8þ cells, one group of vaccinated animals was treated with anti-CD8 mAb. Infection was followed for 8 weeks. The vaccinated CD8þ-depleted group developed smaller lesions than the non-depleted group. CD8 depletion did not affect tissue parasitism or antibody response against the parasite, and treated animals displayed milder inflammation and better tissue integrity. IFN-g production in spleen and draining lymph node was impaired in the depleted group, suggesting that CD8þ cells produced this cytokine in IL-12-independent vaccination. Such results suggest that this T cell subset contributes to augmented pathology in IL12/23p40_/_ mice vaccinated and challenged with L. amazonensis. Although these cells could produce some IFN-g the in the absence of IL-12, they do not affect the parasite tissue load. |
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Hernández Sanabria, Mayra XiomaraAfonso, Luís Carlos CroccoGolgher, DeniseTafuri, Wagner LuizVieira, Leda Quercia2015-03-13T13:16:06Z2015-03-13T13:16:06Z2007HERNÁNDEZ SANABRIA, M. X. et al. CD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice. Microbes and Infection, v. 9, p. 1124-1134, 2007. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1286457907001979>. Acesso em: 08 nov. 2014.1286-4579http://www.repositorio.ufop.br/handle/123456789/4629https://doi.org/10.1016/j.micinf.2007.05.016Vaccine-induced protection against leishmaniasis is largely dependent on cell-mediated type 1 response and IL-12-driven IFN-g production. Surprisingly, our previous data showed that IL-12/23p40_/_ mice could be vaccinated against L. amazonensis and were able to produce limited amounts of IFN-g. Since the role of CD8þT in immunization against L. amazonensis is obscure, the aim of this study was to evaluate the effects of CD8þ cells in protection against L. amazonensis in IL-12/23p40_/_ mice. In order to deplete CD8þ cells, one group of vaccinated animals was treated with anti-CD8 mAb. Infection was followed for 8 weeks. The vaccinated CD8þ-depleted group developed smaller lesions than the non-depleted group. CD8 depletion did not affect tissue parasitism or antibody response against the parasite, and treated animals displayed milder inflammation and better tissue integrity. IFN-g production in spleen and draining lymph node was impaired in the depleted group, suggesting that CD8þ cells produced this cytokine in IL-12-independent vaccination. Such results suggest that this T cell subset contributes to augmented pathology in IL12/23p40_/_ mice vaccinated and challenged with L. amazonensis. Although these cells could produce some IFN-g the in the absence of IL-12, they do not affect the parasite tissue load.LeishmaniaProtozoaParasiteVaccineAdjuvantCD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Microbes and Infection concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3530901314555.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-82636http://www.repositorio.ufop.br/bitstream/123456789/4629/2/license.txtc2ffdd99e58acf69202dff00d361f23aMD52ORIGINALARTIGO_CD CellsRequired.pdfARTIGO_CD CellsRequired.pdfapplication/pdf1582428http://www.repositorio.ufop.br/bitstream/123456789/4629/1/ARTIGO_CD%20CellsRequired.pdf357a59007351144cc6e43f9381ae6cb5MD51123456789/46292019-06-24 15:25:45.809oai:localhost:123456789/4629PGh0bWw+Cjxib2R5Pgo8ZGl2IGFsaWduPSJqdXN0aWZ5Ij48c3Ryb25nPkxpY2VuJmNjZWRpbDthIGRvIFJlcG9zaXQmb2FjdXRlO3JpbyBJbnN0aXR1Y2lvbmFsIGRhIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRlIE91cm8gUHJldG88L3N0cm9uZz4KICA8YnI+CiAgPGJyPgogIEFvIGNvbmNvcmRhciBjb20gZXN0YSBsaWNlbiZjY2VkaWw7YSwgdm9jJmVjaXJjOyhzKSBhdXRvcihlcykgb3UgdGl0dWxhcihlcykgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIGRhIG9icmEgYXF1aSBkZXNjcml0YSBjb25jZWRlKG0pICZhZ3JhdmU7CiAgPGJyPgogIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRlIE91cm8gUHJldG8gKFVGT1ApIGdlc3RvcmEgZG8gUmVwb3NpdCZvYWN1dGU7cmlvIEluc3RpdHVjaW9uYWwgZGEgVW5pdmVyc2lkYWRlIEZlZGVyYWwgZGUgT3VybyBQcmV0bwogIDxicj4KICAoUkktVUZPUCksIG8gZGlyZWl0byBuJmF0aWxkZTtvLWV4Y2x1c2l2byBkZSByZXByb2R1emlyLCBjb252ZXJ0ZXIgKGNvbW8gZGVmaW5pZG8gYWJhaXhvKSBlL291IGRpc3RyaWJ1aXIgbyBkb2N1bWVudG8gZGVwb3NpdGFkbwogIDxicj4KICBlbSBmb3JtYXRvIGltcHJlc3NvLCBlbGV0ciZvY2lyYztuaWNvIG91IGVtIHF1YWxxdWVyIG91dHJvIG1laW8uCiAgPGJyPgogIDxicj4KICBWb2MmZWNpcmM7KHMpIGNvbmNvcmRhKG0pIHF1ZSBhIFVGT1AsIGdlc3RvcmEgZG8gUkktVUZPUCwgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZSZ1YWN1dGU7ZG8sIGNvbnZlcnRlciBvIGFycXVpdm8gZGVwb3NpdGFkbyBhCiAgPGJyPgogIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBjb20gZmlucyBkZSBwcmVzZXJ2YSZjY2VkaWw7JmF0aWxkZTtvLiBWb2MmZWNpcmM7KHMpIHRhbWImZWFjdXRlO20gY29uY29yZGEobSkgcXVlIGEgVUZPUCwgZ2VzdG9yYSBkbyBSSS1VRk9QLCBwb2RlCiAgPGJyPgogIG1hbnRlciBtYWlzIGRlIHVtYSBjJm9hY3V0ZTtwaWEgZGVzdGUgZGVwJm9hY3V0ZTtzaXRvIHBhcmEgZmlucyBkZSBzZWd1cmFuJmNjZWRpbDthLCA8ZW0+YmFjay11cDwvZW0+IGUvb3UgcHJlc2VydmEmY2NlZGlsOyZhdGlsZGU7by4KICA8YnI+CiAgPGJyPgogIFZvYyZlY2lyYzsocykgZGVjbGFyYShtKSBxdWUgYSBhcHJlc2VudGEmY2NlZGlsOyZhdGlsZGU7byBkbyBzZXUgdHJhYmFsaG8gJmVhY3V0ZTsgb3JpZ2luYWwgZSBxdWUgdm9jJmVjaXJjOyhzKSBwb2RlKG0pIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zCiAgPGJyPgogIG5lc3RhIGxpY2VuJmNjZWRpbDthLiBWb2MmZWNpcmM7KHMpIHRhbWImZWFjdXRlO20gZGVjbGFyYShtKSBxdWUgbyBlbnZpbyAmZWFjdXRlOyBkZSBzZXUgY29uaGVjaW1lbnRvIGUgbiZhdGlsZGU7byBpbmZyaW5nZSBvcyBkaXJlaXRvcyBhdXRvcmFpcyBkZSBvdXRyYQogIDxicj4KICBwZXNzb2Egb3UgaW5zdGl0dWkmY2NlZGlsOyZhdGlsZGU7by4gQ2FzbyBvIGRvY3VtZW50byBhIHNlciBkZXBvc2l0YWRvIGNvbnRlbmhhIG1hdGVyaWFsIHBhcmEgbyBxdWFsIHZvYyZlY2lyYzsocykgbiZhdGlsZGU7byBkZXQmZWFjdXRlO20gYSB0aXR1bGFyaWRhZGUKICA8YnI+CiAgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2MmZWNpcmM7KHMpIGRlY2xhcmEobSkgcXVlIG9idGV2ZSBhIHBlcm1pc3MmYXRpbGRlO28gaXJyZXN0cml0YSBkbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkZSBjb25jZWRlciAmYWdyYXZlOwogIDxicj4KICBVRk9QLCBnZXN0b3JhIGRvIFJJLVVGT1Agb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBsaWNlbiZjY2VkaWw7YSBlIHF1ZSBvcyBtYXRlcmlhaXMgZGUgcHJvcHJpZWRhZGUgZGUgdGVyY2Vpcm9zLCBlc3QmYXRpbGRlO28KICA8YnI+CiAgZGV2aWRhbWVudGUgaWRlbnRpZmljYWRvcyBlIHJlY29uaGVjaWRvcyBubyB0ZXh0byBvdSBjb250ZSZ1YWN1dGU7ZG8gZGEgYXByZXNlbnRhJmNjZWRpbDsmYXRpbGRlO28uCiAgPGJyPgogIDxicj4KICBDQVNPIE8gVFJBQkFMSE8gREVQT1NJVEFETyBURU5IQSBTSURPIEZJTkFOQ0lBRE8gT1UgQVBPSUFETyBQT1IgVU0gJk9hY3V0ZTtSRyZBdGlsZGU7TywgUVVFIE4mQXRpbGRlO08gQSBJTlNUSVRVSSZDY2VkaWw7JkF0aWxkZTtPIERFU1RFCiAgPGJyPgogIFJFU1BPU0lUJk9hY3V0ZTtSSU86IFZPQyZFY2lyYzsgREVDTEFSQSBURVIgQ1VNUFJJRE8gVE9ET1MgT1MgRElSRUlUT1MgREUgUkVWSVMmQXRpbGRlO08gRSBRVUFJU1FVRVIgT1VUUkFTIE9CUklHQSZDY2VkaWw7Jk90aWxkZTtFUwogIDxicj4KICBSRVFVRVJJREFTIFBFTE8gQ09OVFJBVE8gT1UgQUNPUkRPLiAKICA8YnI+CiAgPGJyPgogIE8gcmVwb3NpdCZvYWN1dGU7cmlvIGlkZW50aWZpY2FyJmFhY3V0ZTsgY2xhcmFtZW50ZSBvIHNldShzKSBub21lKHMpIGNvbW8gYXV0b3IoZXMpIG91IHRpdHVsYXIoZXMpIGRvIGRpcmVpdG8gZGUgYXV0b3IoZXMpIGRvIGRvY3VtZW50bwogIDxicj4KICBzdWJtZXRpZG8gZSBkZWNsYXJhIHF1ZSBuJmF0aWxkZTtvIGZhciZhYWN1dGU7IHF1YWxxdWVyIGFsdGVyYSZjY2VkaWw7JmF0aWxkZTtvIGFsJmVhY3V0ZTttIGRhcyBwZXJtaXRpZGFzIHBvciBlc3RhIGxpY2VuJmNjZWRpbDthLjwvcD4KPC9kaXY+CjwvYm9keT4KPC9odG1sPgo=Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-06-24T19:25:45Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
CD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice. |
title |
CD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice. |
spellingShingle |
CD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice. Hernández Sanabria, Mayra Xiomara Leishmania Protozoa Parasite Vaccine Adjuvant |
title_short |
CD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice. |
title_full |
CD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice. |
title_fullStr |
CD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice. |
title_full_unstemmed |
CD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice. |
title_sort |
CD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice. |
author |
Hernández Sanabria, Mayra Xiomara |
author_facet |
Hernández Sanabria, Mayra Xiomara Afonso, Luís Carlos Crocco Golgher, Denise Tafuri, Wagner Luiz Vieira, Leda Quercia |
author_role |
author |
author2 |
Afonso, Luís Carlos Crocco Golgher, Denise Tafuri, Wagner Luiz Vieira, Leda Quercia |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Hernández Sanabria, Mayra Xiomara Afonso, Luís Carlos Crocco Golgher, Denise Tafuri, Wagner Luiz Vieira, Leda Quercia |
dc.subject.por.fl_str_mv |
Leishmania Protozoa Parasite Vaccine Adjuvant |
topic |
Leishmania Protozoa Parasite Vaccine Adjuvant |
description |
Vaccine-induced protection against leishmaniasis is largely dependent on cell-mediated type 1 response and IL-12-driven IFN-g production. Surprisingly, our previous data showed that IL-12/23p40_/_ mice could be vaccinated against L. amazonensis and were able to produce limited amounts of IFN-g. Since the role of CD8þT in immunization against L. amazonensis is obscure, the aim of this study was to evaluate the effects of CD8þ cells in protection against L. amazonensis in IL-12/23p40_/_ mice. In order to deplete CD8þ cells, one group of vaccinated animals was treated with anti-CD8 mAb. Infection was followed for 8 weeks. The vaccinated CD8þ-depleted group developed smaller lesions than the non-depleted group. CD8 depletion did not affect tissue parasitism or antibody response against the parasite, and treated animals displayed milder inflammation and better tissue integrity. IFN-g production in spleen and draining lymph node was impaired in the depleted group, suggesting that CD8þ cells produced this cytokine in IL-12-independent vaccination. Such results suggest that this T cell subset contributes to augmented pathology in IL12/23p40_/_ mice vaccinated and challenged with L. amazonensis. Although these cells could produce some IFN-g the in the absence of IL-12, they do not affect the parasite tissue load. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007 |
dc.date.accessioned.fl_str_mv |
2015-03-13T13:16:06Z |
dc.date.available.fl_str_mv |
2015-03-13T13:16:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
HERNÁNDEZ SANABRIA, M. X. et al. CD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice. Microbes and Infection, v. 9, p. 1124-1134, 2007. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1286457907001979>. Acesso em: 08 nov. 2014. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/4629 |
dc.identifier.issn.none.fl_str_mv |
1286-4579 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.micinf.2007.05.016 |
identifier_str_mv |
HERNÁNDEZ SANABRIA, M. X. et al. CD8þT cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40_/_ C57BL/6 mice. Microbes and Infection, v. 9, p. 1124-1134, 2007. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1286457907001979>. Acesso em: 08 nov. 2014. 1286-4579 |
url |
http://www.repositorio.ufop.br/handle/123456789/4629 https://doi.org/10.1016/j.micinf.2007.05.016 |
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eng |
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