Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype.
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Publication Date: | 2017 |
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Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UFOP |
Download full: | http://www.repositorio.ufop.br/handle/123456789/9224 https://doi.org/10.1016/j.actatropica.2016.12.015 |
Summary: | This study was designed to verify the relationship between IgG antibodies isotypes and myocarditis inTrypanosoma cruzi infection using mice and dogs infected with different T. cruzi strains. The animals wereinfected with benznidazole-susceptible Berenice-78 and benznidazole-resistant AAS and VL-10 strains.The IgG subtypes were measured in serum samples from dogs (IgG, IgG1, and IgG2) and mice (IgG, IgG1,IgG2a, and IgG2b). The infection of dogs with VL-10 strain induced the highest levels of heart inflammationwhile intermediate and lower levels were detected with Berenice-78 and AAS strains, respectively. Similarresults were found in mice infected with VL-10, but not in those infected with AAS or Berenice-78 strains.The AAS strain induced higher levels of heart inflammation in mice, while Berenice-78 strain was notable to induce it. Correlation analysis between myocarditis and antibody reactivity index revealed veryinteresting results, mainly for IgG and IgG1, the latter being the most exciting. High IgG1 showed asignificant correlation with myocarditis in both experimental models, being more significant in dogs(r = 0.94, p < 0.0001) than in mice (r = 0.58, p = 0.047). Overall, our data suggest that IgG1 could be a goodmarker to demonstrate myocarditis intensity in Chagas disease.© |
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Caldas, Ivo SantanaDiniz, Lívia de FigueiredoGuedes, Paulo Marcos da MattaNascimento, Álvaro Fernando da Silva doGalvão, Lúcia Maria da CunhaLima, Wanderson Geraldo deCaldas, SérgioBahia, Maria Terezinha2017-12-20T15:05:36Z2017-12-20T15:05:36Z2017CALDAS, I. S. et al. Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype. Acta Tropica, v. 167, p. 40-49, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0001706X16307100>. Acesso em: 15 set. 2017.0001-706Xhttp://www.repositorio.ufop.br/handle/123456789/9224https://doi.org/10.1016/j.actatropica.2016.12.015This study was designed to verify the relationship between IgG antibodies isotypes and myocarditis inTrypanosoma cruzi infection using mice and dogs infected with different T. cruzi strains. The animals wereinfected with benznidazole-susceptible Berenice-78 and benznidazole-resistant AAS and VL-10 strains.The IgG subtypes were measured in serum samples from dogs (IgG, IgG1, and IgG2) and mice (IgG, IgG1,IgG2a, and IgG2b). The infection of dogs with VL-10 strain induced the highest levels of heart inflammationwhile intermediate and lower levels were detected with Berenice-78 and AAS strains, respectively. Similarresults were found in mice infected with VL-10, but not in those infected with AAS or Berenice-78 strains.The AAS strain induced higher levels of heart inflammation in mice, while Berenice-78 strain was notable to induce it. Correlation analysis between myocarditis and antibody reactivity index revealed veryinteresting results, mainly for IgG and IgG1, the latter being the most exciting. High IgG1 showed asignificant correlation with myocarditis in both experimental models, being more significant in dogs(r = 0.94, p < 0.0001) than in mice (r = 0.58, p = 0.047). Overall, our data suggest that IgG1 could be a goodmarker to demonstrate myocarditis intensity in Chagas disease.©O periódico Acta Tropica concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 4210820142876.info:eu-repo/semantics/openAccessChagas diseaseImmunopathogenesisMyocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/9224/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_MyocarditisDifferentExperimental.pdfARTIGO_MyocarditisDifferentExperimental.pdfapplication/pdf1864218http://www.repositorio.ufop.br/bitstream/123456789/9224/1/ARTIGO_MyocarditisDifferentExperimental.pdf611450516d60a22245c3bc39da5fec48MD51123456789/92242020-02-20 09:49:04.408oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332020-02-20T14:49:04Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype. |
title |
Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype. |
spellingShingle |
Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype. Caldas, Ivo Santana Chagas disease Immunopathogenesis |
title_short |
Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype. |
title_full |
Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype. |
title_fullStr |
Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype. |
title_full_unstemmed |
Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype. |
title_sort |
Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype. |
author |
Caldas, Ivo Santana |
author_facet |
Caldas, Ivo Santana Diniz, Lívia de Figueiredo Guedes, Paulo Marcos da Matta Nascimento, Álvaro Fernando da Silva do Galvão, Lúcia Maria da Cunha Lima, Wanderson Geraldo de Caldas, Sérgio Bahia, Maria Terezinha |
author_role |
author |
author2 |
Diniz, Lívia de Figueiredo Guedes, Paulo Marcos da Matta Nascimento, Álvaro Fernando da Silva do Galvão, Lúcia Maria da Cunha Lima, Wanderson Geraldo de Caldas, Sérgio Bahia, Maria Terezinha |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Caldas, Ivo Santana Diniz, Lívia de Figueiredo Guedes, Paulo Marcos da Matta Nascimento, Álvaro Fernando da Silva do Galvão, Lúcia Maria da Cunha Lima, Wanderson Geraldo de Caldas, Sérgio Bahia, Maria Terezinha |
dc.subject.por.fl_str_mv |
Chagas disease Immunopathogenesis |
topic |
Chagas disease Immunopathogenesis |
description |
This study was designed to verify the relationship between IgG antibodies isotypes and myocarditis inTrypanosoma cruzi infection using mice and dogs infected with different T. cruzi strains. The animals wereinfected with benznidazole-susceptible Berenice-78 and benznidazole-resistant AAS and VL-10 strains.The IgG subtypes were measured in serum samples from dogs (IgG, IgG1, and IgG2) and mice (IgG, IgG1,IgG2a, and IgG2b). The infection of dogs with VL-10 strain induced the highest levels of heart inflammationwhile intermediate and lower levels were detected with Berenice-78 and AAS strains, respectively. Similarresults were found in mice infected with VL-10, but not in those infected with AAS or Berenice-78 strains.The AAS strain induced higher levels of heart inflammation in mice, while Berenice-78 strain was notable to induce it. Correlation analysis between myocarditis and antibody reactivity index revealed veryinteresting results, mainly for IgG and IgG1, the latter being the most exciting. High IgG1 showed asignificant correlation with myocarditis in both experimental models, being more significant in dogs(r = 0.94, p < 0.0001) than in mice (r = 0.58, p = 0.047). Overall, our data suggest that IgG1 could be a goodmarker to demonstrate myocarditis intensity in Chagas disease.© |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-12-20T15:05:36Z |
dc.date.available.fl_str_mv |
2017-12-20T15:05:36Z |
dc.date.issued.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CALDAS, I. S. et al. Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype. Acta Tropica, v. 167, p. 40-49, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0001706X16307100>. Acesso em: 15 set. 2017. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/9224 |
dc.identifier.issn.none.fl_str_mv |
0001-706X |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.actatropica.2016.12.015 |
identifier_str_mv |
CALDAS, I. S. et al. Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype. Acta Tropica, v. 167, p. 40-49, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0001706X16307100>. Acesso em: 15 set. 2017. 0001-706X |
url |
http://www.repositorio.ufop.br/handle/123456789/9224 https://doi.org/10.1016/j.actatropica.2016.12.015 |
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eng |
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info:eu-repo/semantics/openAccess |
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