Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach.
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/8899 https://doi.org/10.1128/CVI.00023-13 |
Resumo: | In Brazil, the percentage of infected dogs living in areas where canine visceral leishmaniasis (CVL) is endemic ranges from 10 to 62%; however, the prevalence of infection in dogs is probably higher than figures reported from serological studies. In addition, problems with the occurrence of false-positive or false-negative results in the serodiagnosis of CVL have been reported. The present work analyzed the potential of synthetic peptides mapped from hypothetical proteins for improvement of the serodiagnosis of Leishmania infantum infection in dogs. From 26 identified leishmanial proteins, eight were selected, considering that no homologies between these proteins and others from trypanosomatide sequence databases were encountered. The sequences of these proteins were mapped to identify linear B-cell epitopes, and 17 peptides were synthesized and tested in enzyme-linked immunosorbent assays (ELISAs) for the serodiagnosis of L. infantum infection in dogs. Of these, three exhibited sensitivity and specificity values higher than 75% and 90%, respectively, to differentiate L. infantum-infected animals from Trypanosoma cruziinfected animals and healthy animals. Soluble Leishmania antigen (SLA) showed poor sensitivity (4%) and specificity (36%) to differentiate L. infantum-infected dogs from healthy and T. cruzi-infected dogs. Lastly, the three selected peptides were combined in different mixtures and higher sensitivity and specificity values were obtained, even when sera from T. cruzi-infected dogs were used. The study’s findings suggest that these three peptides can constitute a potential tool for more sensitive and specific serodiagnosis of L. infantum infection in dogs. |
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Chávez Fumagalli, Miguel AngelMartins, Vívian TamiettiTestasicca, Miriam Conceição de SouzaLage, Daniela PagliaraCosta, Lourena EmanueleLage, Paula SouzaDuarte, Mariana CostaKer, Henrique GamaRibeiro, Tatiana GomesCarvalho, Fernando A. A.Régis, Wiliam César BentoReis, Alexandre BarbosaTavares, Carlos Alberto PereiraSoto, ManuelFernandes, Ana PaulaCoelho, Eduardo Antônio Ferraz2017-10-09T13:44:33Z2017-10-09T13:44:33Z2013CHÁVEZ FUMAGALLI, M. A. et al. Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach. Clinical and Vaccine Immunology, v. 20, p. 835-841, 2013. Disponível em: <http://cvi.asm.org/content/20/6/835.figures-only>. Acesso em: 10 nov. 2016.1556-679Xhttp://www.repositorio.ufop.br/handle/123456789/8899https://doi.org/10.1128/CVI.00023-13In Brazil, the percentage of infected dogs living in areas where canine visceral leishmaniasis (CVL) is endemic ranges from 10 to 62%; however, the prevalence of infection in dogs is probably higher than figures reported from serological studies. In addition, problems with the occurrence of false-positive or false-negative results in the serodiagnosis of CVL have been reported. The present work analyzed the potential of synthetic peptides mapped from hypothetical proteins for improvement of the serodiagnosis of Leishmania infantum infection in dogs. From 26 identified leishmanial proteins, eight were selected, considering that no homologies between these proteins and others from trypanosomatide sequence databases were encountered. The sequences of these proteins were mapped to identify linear B-cell epitopes, and 17 peptides were synthesized and tested in enzyme-linked immunosorbent assays (ELISAs) for the serodiagnosis of L. infantum infection in dogs. Of these, three exhibited sensitivity and specificity values higher than 75% and 90%, respectively, to differentiate L. infantum-infected animals from Trypanosoma cruziinfected animals and healthy animals. Soluble Leishmania antigen (SLA) showed poor sensitivity (4%) and specificity (36%) to differentiate L. infantum-infected dogs from healthy and T. cruzi-infected dogs. Lastly, the three selected peptides were combined in different mixtures and higher sensitivity and specificity values were obtained, even when sera from T. cruzi-infected dogs were used. The study’s findings suggest that these three peptides can constitute a potential tool for more sensitive and specific serodiagnosis of L. infantum infection in dogs.Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/8899/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_SensitiveSpecificSerodiagnosis.pdfARTIGO_SensitiveSpecificSerodiagnosis.pdfapplication/pdf516434http://www.repositorio.ufop.br/bitstream/123456789/8899/1/ARTIGO_SensitiveSpecificSerodiagnosis.pdf0c3f4b21f7fa9a9f9f17ab005e31a34cMD51123456789/88992020-02-06 06:06:02.407oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332020-02-06T11:06:02Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach. |
title |
Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach. |
spellingShingle |
Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach. Chávez Fumagalli, Miguel Angel |
title_short |
Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach. |
title_full |
Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach. |
title_fullStr |
Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach. |
title_full_unstemmed |
Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach. |
title_sort |
Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach. |
author |
Chávez Fumagalli, Miguel Angel |
author_facet |
Chávez Fumagalli, Miguel Angel Martins, Vívian Tamietti Testasicca, Miriam Conceição de Souza Lage, Daniela Pagliara Costa, Lourena Emanuele Lage, Paula Souza Duarte, Mariana Costa Ker, Henrique Gama Ribeiro, Tatiana Gomes Carvalho, Fernando A. A. Régis, Wiliam César Bento Reis, Alexandre Barbosa Tavares, Carlos Alberto Pereira Soto, Manuel Fernandes, Ana Paula Coelho, Eduardo Antônio Ferraz |
author_role |
author |
author2 |
Martins, Vívian Tamietti Testasicca, Miriam Conceição de Souza Lage, Daniela Pagliara Costa, Lourena Emanuele Lage, Paula Souza Duarte, Mariana Costa Ker, Henrique Gama Ribeiro, Tatiana Gomes Carvalho, Fernando A. A. Régis, Wiliam César Bento Reis, Alexandre Barbosa Tavares, Carlos Alberto Pereira Soto, Manuel Fernandes, Ana Paula Coelho, Eduardo Antônio Ferraz |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Chávez Fumagalli, Miguel Angel Martins, Vívian Tamietti Testasicca, Miriam Conceição de Souza Lage, Daniela Pagliara Costa, Lourena Emanuele Lage, Paula Souza Duarte, Mariana Costa Ker, Henrique Gama Ribeiro, Tatiana Gomes Carvalho, Fernando A. A. Régis, Wiliam César Bento Reis, Alexandre Barbosa Tavares, Carlos Alberto Pereira Soto, Manuel Fernandes, Ana Paula Coelho, Eduardo Antônio Ferraz |
description |
In Brazil, the percentage of infected dogs living in areas where canine visceral leishmaniasis (CVL) is endemic ranges from 10 to 62%; however, the prevalence of infection in dogs is probably higher than figures reported from serological studies. In addition, problems with the occurrence of false-positive or false-negative results in the serodiagnosis of CVL have been reported. The present work analyzed the potential of synthetic peptides mapped from hypothetical proteins for improvement of the serodiagnosis of Leishmania infantum infection in dogs. From 26 identified leishmanial proteins, eight were selected, considering that no homologies between these proteins and others from trypanosomatide sequence databases were encountered. The sequences of these proteins were mapped to identify linear B-cell epitopes, and 17 peptides were synthesized and tested in enzyme-linked immunosorbent assays (ELISAs) for the serodiagnosis of L. infantum infection in dogs. Of these, three exhibited sensitivity and specificity values higher than 75% and 90%, respectively, to differentiate L. infantum-infected animals from Trypanosoma cruziinfected animals and healthy animals. Soluble Leishmania antigen (SLA) showed poor sensitivity (4%) and specificity (36%) to differentiate L. infantum-infected dogs from healthy and T. cruzi-infected dogs. Lastly, the three selected peptides were combined in different mixtures and higher sensitivity and specificity values were obtained, even when sera from T. cruzi-infected dogs were used. The study’s findings suggest that these three peptides can constitute a potential tool for more sensitive and specific serodiagnosis of L. infantum infection in dogs. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2017-10-09T13:44:33Z |
dc.date.available.fl_str_mv |
2017-10-09T13:44:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CHÁVEZ FUMAGALLI, M. A. et al. Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach. Clinical and Vaccine Immunology, v. 20, p. 835-841, 2013. Disponível em: <http://cvi.asm.org/content/20/6/835.figures-only>. Acesso em: 10 nov. 2016. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/8899 |
dc.identifier.issn.none.fl_str_mv |
1556-679X |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1128/CVI.00023-13 |
identifier_str_mv |
CHÁVEZ FUMAGALLI, M. A. et al. Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach. Clinical and Vaccine Immunology, v. 20, p. 835-841, 2013. Disponível em: <http://cvi.asm.org/content/20/6/835.figures-only>. Acesso em: 10 nov. 2016. 1556-679X |
url |
http://www.repositorio.ufop.br/handle/123456789/8899 https://doi.org/10.1128/CVI.00023-13 |
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eng |
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eng |
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openAccess |
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