Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices.

Detalhes bibliográficos
Autor(a) principal: Prado, Marco Antônio Maximo
Data de Publicação: 1990
Outros Autores: Santos, T. Moraes, Freitas, Renata Nascimento de, Silva, Marco Aurélio Romano, Gomez, Marcus Vinicius
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/4916
https://doi.org/10.1016/0165-0270(90)90110-2
Resumo: A chemiluminescent method has been used recently for the determination of acetylcholine with limitations such as the presence of a cholinesterase inhibitor in the incubation medium, which is indispensable for the study of acetylcholine release by various agents. A modified procedure is presented in which the cholinesterase inhibitor eserine (physostigmine) is extracted from the medium. The results showed complete recovery when labelled acetylcholine was used. This modified procedure was used to determine the release of acetylcholine evoked by tityustoxin and ouabain. The results were comparable to those obtained by bioassay using a strip of guinea pig ileum.
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spelling Prado, Marco Antônio MaximoSantos, T. MoraesFreitas, Renata Nascimento deSilva, Marco Aurélio RomanoGomez, Marcus Vinicius2015-04-01T17:30:11Z2015-04-01T17:30:11Z1990PRADO, M. A. M. et al. Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices. Journal of Neuroscience Methods, v. 31, p. 193-196, 1990. Disponível em: <https://www.sciencedirect.com/science/article/pii/0165027090901102>. Acesso em: 08 nov. 2014.0165-0270http://www.repositorio.ufop.br/handle/123456789/4916https://doi.org/10.1016/0165-0270(90)90110-2A chemiluminescent method has been used recently for the determination of acetylcholine with limitations such as the presence of a cholinesterase inhibitor in the incubation medium, which is indispensable for the study of acetylcholine release by various agents. A modified procedure is presented in which the cholinesterase inhibitor eserine (physostigmine) is extracted from the medium. The results showed complete recovery when labelled acetylcholine was used. This modified procedure was used to determine the release of acetylcholine evoked by tityustoxin and ouabain. The results were comparable to those obtained by bioassay using a strip of guinea pig ileum.Acetylcholine assayChemiluminescent procedureNeurotransmitter releaseCholine oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Journal of Neuroscience Methods concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3556580424364.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-82636http://www.repositorio.ufop.br/bitstream/123456789/4916/2/license.txtc2ffdd99e58acf69202dff00d361f23aMD52ORIGINALARTIGO_CholineOxidaseChemiluminescent.pdfARTIGO_CholineOxidaseChemiluminescent.pdfapplication/pdf298809http://www.repositorio.ufop.br/bitstream/123456789/4916/1/ARTIGO_CholineOxidaseChemiluminescent.pdfa1df56eed32e0a15d2a8f42ca85636e4MD51123456789/49162019-07-04 10:24:12.505oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-07-04T14:24:12Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.pt_BR.fl_str_mv Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices.
title Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices.
spellingShingle Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices.
Prado, Marco Antônio Maximo
Acetylcholine assay
Chemiluminescent procedure
Neurotransmitter release
title_short Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices.
title_full Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices.
title_fullStr Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices.
title_full_unstemmed Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices.
title_sort Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices.
author Prado, Marco Antônio Maximo
author_facet Prado, Marco Antônio Maximo
Santos, T. Moraes
Freitas, Renata Nascimento de
Silva, Marco Aurélio Romano
Gomez, Marcus Vinicius
author_role author
author2 Santos, T. Moraes
Freitas, Renata Nascimento de
Silva, Marco Aurélio Romano
Gomez, Marcus Vinicius
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Prado, Marco Antônio Maximo
Santos, T. Moraes
Freitas, Renata Nascimento de
Silva, Marco Aurélio Romano
Gomez, Marcus Vinicius
dc.subject.por.fl_str_mv Acetylcholine assay
Chemiluminescent procedure
Neurotransmitter release
topic Acetylcholine assay
Chemiluminescent procedure
Neurotransmitter release
description A chemiluminescent method has been used recently for the determination of acetylcholine with limitations such as the presence of a cholinesterase inhibitor in the incubation medium, which is indispensable for the study of acetylcholine release by various agents. A modified procedure is presented in which the cholinesterase inhibitor eserine (physostigmine) is extracted from the medium. The results showed complete recovery when labelled acetylcholine was used. This modified procedure was used to determine the release of acetylcholine evoked by tityustoxin and ouabain. The results were comparable to those obtained by bioassay using a strip of guinea pig ileum.
publishDate 1990
dc.date.issued.fl_str_mv 1990
dc.date.accessioned.fl_str_mv 2015-04-01T17:30:11Z
dc.date.available.fl_str_mv 2015-04-01T17:30:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PRADO, M. A. M. et al. Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices. Journal of Neuroscience Methods, v. 31, p. 193-196, 1990. Disponível em: <https://www.sciencedirect.com/science/article/pii/0165027090901102>. Acesso em: 08 nov. 2014.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufop.br/handle/123456789/4916
dc.identifier.issn.none.fl_str_mv 0165-0270
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/0165-0270(90)90110-2
identifier_str_mv PRADO, M. A. M. et al. Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices. Journal of Neuroscience Methods, v. 31, p. 193-196, 1990. Disponível em: <https://www.sciencedirect.com/science/article/pii/0165027090901102>. Acesso em: 08 nov. 2014.
0165-0270
url http://www.repositorio.ufop.br/handle/123456789/4916
https://doi.org/10.1016/0165-0270(90)90110-2
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
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collection Repositório Institucional da UFOP
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