Histological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis.
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/971 |
Resumo: | Vaccine candidates, including live and/or killed parasites, Leishmania -purified fractions, defined recombinant antigens and antigen-encoding DNA-plasmids have been proposed to use as vaccine anti- Leishmania . More recently, the hamsters have been used to pre selection of antigens candidate to apply in further experiments using canine model. In this report we evaluated the kinetics of cell migration in dermal inflammatory infiltrate, circulating leukocytes and the presence of nitric oxide (NO)/induced nitric oxide synthase during the early (1–24 h) and late (48–168 h) periods following inoculation of hamsters with antigenic components of anti-canine visceral leishmaniasis vaccines Leishmune 1 and Leishmania braziliensis antigen (LB) with and without saponin (Sap) adjuvant. Our results show that LB caused an early reduction of lymphocytes in the dermis while Sap and LBSap triggered a late recruitment, suggesting the role of the adjuvant in the traffic of antigen-presenting cells and the induction of lymphocyte migration. In that manner our results suggest that the kinetics of cell migration on hamster model may be of value in the selection of vaccine antigens prior the tests in dogs particularly in respect of the toxicity of the preparations. |
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Moreira, Nádia das DoresGiunchetti, Rodolfo CordeiroCarneiro, Cláudia MartinsSouza, Juliana Vitoriano deRoatt, Bruno MendesMalaquias, Luiz Cosme CottaOliveira, Rodrigo Corrêa deReis, Alexandre Barbosa2012-07-04T18:41:25Z2012-07-04T18:41:25Z2009MOREIRA, N. das. D. et al. Histological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis. Veterinary Immunology and Immunopathology, v. 128, n. 4, p. 418-424, 2009. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0165242708007708>. Acesso em: 04 jul. 2012.01652427http://www.repositorio.ufop.br/handle/123456789/971Vaccine candidates, including live and/or killed parasites, Leishmania -purified fractions, defined recombinant antigens and antigen-encoding DNA-plasmids have been proposed to use as vaccine anti- Leishmania . More recently, the hamsters have been used to pre selection of antigens candidate to apply in further experiments using canine model. In this report we evaluated the kinetics of cell migration in dermal inflammatory infiltrate, circulating leukocytes and the presence of nitric oxide (NO)/induced nitric oxide synthase during the early (1–24 h) and late (48–168 h) periods following inoculation of hamsters with antigenic components of anti-canine visceral leishmaniasis vaccines Leishmune 1 and Leishmania braziliensis antigen (LB) with and without saponin (Sap) adjuvant. Our results show that LB caused an early reduction of lymphocytes in the dermis while Sap and LBSap triggered a late recruitment, suggesting the role of the adjuvant in the traffic of antigen-presenting cells and the induction of lymphocyte migration. In that manner our results suggest that the kinetics of cell migration on hamster model may be of value in the selection of vaccine antigens prior the tests in dogs particularly in respect of the toxicity of the preparations.HistopathologyInflammation and cell migrationHamsterLeishmania - vaccines antingensSaponin adjuvantHistological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Veterinary Immunology and Immunopathology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3281420495198.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/971/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-820325http://www.repositorio.ufop.br/bitstream/123456789/971/4/license_rdfc7f3e3c064c19e93e905bbb07856484cMD54ORIGINALARTIGO_HistologicalStudyCell.pdfARTIGO_HistologicalStudyCell.pdfapplication/pdf1198009http://www.repositorio.ufop.br/bitstream/123456789/971/1/ARTIGO_HistologicalStudyCell.pdf2dbdc33988629e7e2050b7c556e886c2MD51123456789/9712019-02-25 11:10:13.597oai:localhost:123456789/971Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-02-25T16:10:13Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Histological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis. |
title |
Histological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis. |
spellingShingle |
Histological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis. Moreira, Nádia das Dores Histopathology Inflammation and cell migration Hamster Leishmania - vaccines antingens Saponin adjuvant |
title_short |
Histological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis. |
title_full |
Histological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis. |
title_fullStr |
Histological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis. |
title_full_unstemmed |
Histological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis. |
title_sort |
Histological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis. |
author |
Moreira, Nádia das Dores |
author_facet |
Moreira, Nádia das Dores Giunchetti, Rodolfo Cordeiro Carneiro, Cláudia Martins Souza, Juliana Vitoriano de Roatt, Bruno Mendes Malaquias, Luiz Cosme Cotta Oliveira, Rodrigo Corrêa de Reis, Alexandre Barbosa |
author_role |
author |
author2 |
Giunchetti, Rodolfo Cordeiro Carneiro, Cláudia Martins Souza, Juliana Vitoriano de Roatt, Bruno Mendes Malaquias, Luiz Cosme Cotta Oliveira, Rodrigo Corrêa de Reis, Alexandre Barbosa |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Moreira, Nádia das Dores Giunchetti, Rodolfo Cordeiro Carneiro, Cláudia Martins Souza, Juliana Vitoriano de Roatt, Bruno Mendes Malaquias, Luiz Cosme Cotta Oliveira, Rodrigo Corrêa de Reis, Alexandre Barbosa |
dc.subject.por.fl_str_mv |
Histopathology Inflammation and cell migration Hamster Leishmania - vaccines antingens Saponin adjuvant |
topic |
Histopathology Inflammation and cell migration Hamster Leishmania - vaccines antingens Saponin adjuvant |
description |
Vaccine candidates, including live and/or killed parasites, Leishmania -purified fractions, defined recombinant antigens and antigen-encoding DNA-plasmids have been proposed to use as vaccine anti- Leishmania . More recently, the hamsters have been used to pre selection of antigens candidate to apply in further experiments using canine model. In this report we evaluated the kinetics of cell migration in dermal inflammatory infiltrate, circulating leukocytes and the presence of nitric oxide (NO)/induced nitric oxide synthase during the early (1–24 h) and late (48–168 h) periods following inoculation of hamsters with antigenic components of anti-canine visceral leishmaniasis vaccines Leishmune 1 and Leishmania braziliensis antigen (LB) with and without saponin (Sap) adjuvant. Our results show that LB caused an early reduction of lymphocytes in the dermis while Sap and LBSap triggered a late recruitment, suggesting the role of the adjuvant in the traffic of antigen-presenting cells and the induction of lymphocyte migration. In that manner our results suggest that the kinetics of cell migration on hamster model may be of value in the selection of vaccine antigens prior the tests in dogs particularly in respect of the toxicity of the preparations. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2012-07-04T18:41:25Z |
dc.date.available.fl_str_mv |
2012-07-04T18:41:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MOREIRA, N. das. D. et al. Histological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis. Veterinary Immunology and Immunopathology, v. 128, n. 4, p. 418-424, 2009. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0165242708007708>. Acesso em: 04 jul. 2012. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/971 |
dc.identifier.issn.none.fl_str_mv |
01652427 |
identifier_str_mv |
MOREIRA, N. das. D. et al. Histological study of cell migration in the dermis of hamsters after immunisation with two different vaccines against visceral leishmaniasis. Veterinary Immunology and Immunopathology, v. 128, n. 4, p. 418-424, 2009. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0165242708007708>. Acesso em: 04 jul. 2012. 01652427 |
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