Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes.
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/979 |
Resumo: | A detailed investigation has been carried out about the serological profiles of groups of dogs experimen-tally infected with metacyclic (MT) or blood (BT) trypomastigotes of Berenice-78 Trypanosoma cruzi strain. Peripheral blood was collected from infected dogs and uninfected controls, weekly during 35 days following the acute phase of infection, and immunoglobulin profiles were determined by ELISA. Dogs infected with BT exhibited unaltered levels of IgG2, increases in IgM, IgE, IgA, IgG and IgG1. In contrast, dogs infected with MT presented unaltered levels of IgE and IgG1 and an increase in IgM, IgA, IgG and IgG2 levels. Compared with the MT group, animals infected with BT showed significant increases in IgM on days 7, 14 and 28, in IgA on days 7, 14 and 21, in IgE on days 7 and 14, in IgG on days 14 and 28, and in IgG1 on days 7, 14 and 21. Parasitemia levels of the infected animals were measured over the same time period. No correlations were found between the immunoglobulin profiles and the parasi-temia levels. The results demonstrated that the inoculum source (BT or MT) influence the immunoglob-ulin isotype profile that may drive distinct outcome of acute canine Chagas disease |
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Vital, Wendel CouraCarneiro, Cláudia MartinsMartins, Helen RodriguesLana, Marta deVeloso, Vanja MariaCarvalho, Andréa Teixeira deBahia, Maria TerezinhaOliveira, Rodrigo Corrêa deMartins Filho, Olindo AssisTafuri, Wagner LuizReis, Alexandre Barbosa2012-07-05T13:54:29Z2012-07-05T13:54:29Z2008VITAL, W. C. et al. Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes. Experimental Parasitology, v. 120, n. 3, p. 269-274, nov. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0014489408001963>. Acesso em: 05 jul. 201200144894http://www.repositorio.ufop.br/handle/123456789/979A detailed investigation has been carried out about the serological profiles of groups of dogs experimen-tally infected with metacyclic (MT) or blood (BT) trypomastigotes of Berenice-78 Trypanosoma cruzi strain. Peripheral blood was collected from infected dogs and uninfected controls, weekly during 35 days following the acute phase of infection, and immunoglobulin profiles were determined by ELISA. Dogs infected with BT exhibited unaltered levels of IgG2, increases in IgM, IgE, IgA, IgG and IgG1. In contrast, dogs infected with MT presented unaltered levels of IgE and IgG1 and an increase in IgM, IgA, IgG and IgG2 levels. Compared with the MT group, animals infected with BT showed significant increases in IgM on days 7, 14 and 28, in IgA on days 7, 14 and 21, in IgE on days 7 and 14, in IgG on days 14 and 28, and in IgG1 on days 7, 14 and 21. Parasitemia levels of the infected animals were measured over the same time period. No correlations were found between the immunoglobulin profiles and the parasi-temia levels. The results demonstrated that the inoculum source (BT or MT) influence the immunoglob-ulin isotype profile that may drive distinct outcome of acute canine Chagas diseaseTrypanosoma cruziChagas' diseaseCanine modelClass and subclass of immunoglobulinsTrypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Experimental Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3281990771185.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/979/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55ORIGINALARTIGO_TrypanossomaCruziImmunoglobulin.pdfARTIGO_TrypanossomaCruziImmunoglobulin.pdfapplication/pdf235411http://www.repositorio.ufop.br/bitstream/123456789/979/1/ARTIGO_TrypanossomaCruziImmunoglobulin.pdfd74d919bb10636ed37a5c2084759317aMD51123456789/9792019-02-25 12:16:44.899oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-02-25T17:16:44Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes. |
title |
Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes. |
spellingShingle |
Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes. Vital, Wendel Coura Trypanosoma cruzi Chagas' disease Canine model Class and subclass of immunoglobulins |
title_short |
Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes. |
title_full |
Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes. |
title_fullStr |
Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes. |
title_full_unstemmed |
Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes. |
title_sort |
Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes. |
author |
Vital, Wendel Coura |
author_facet |
Vital, Wendel Coura Carneiro, Cláudia Martins Martins, Helen Rodrigues Lana, Marta de Veloso, Vanja Maria Carvalho, Andréa Teixeira de Bahia, Maria Terezinha Oliveira, Rodrigo Corrêa de Martins Filho, Olindo Assis Tafuri, Wagner Luiz Reis, Alexandre Barbosa |
author_role |
author |
author2 |
Carneiro, Cláudia Martins Martins, Helen Rodrigues Lana, Marta de Veloso, Vanja Maria Carvalho, Andréa Teixeira de Bahia, Maria Terezinha Oliveira, Rodrigo Corrêa de Martins Filho, Olindo Assis Tafuri, Wagner Luiz Reis, Alexandre Barbosa |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vital, Wendel Coura Carneiro, Cláudia Martins Martins, Helen Rodrigues Lana, Marta de Veloso, Vanja Maria Carvalho, Andréa Teixeira de Bahia, Maria Terezinha Oliveira, Rodrigo Corrêa de Martins Filho, Olindo Assis Tafuri, Wagner Luiz Reis, Alexandre Barbosa |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi Chagas' disease Canine model Class and subclass of immunoglobulins |
topic |
Trypanosoma cruzi Chagas' disease Canine model Class and subclass of immunoglobulins |
description |
A detailed investigation has been carried out about the serological profiles of groups of dogs experimen-tally infected with metacyclic (MT) or blood (BT) trypomastigotes of Berenice-78 Trypanosoma cruzi strain. Peripheral blood was collected from infected dogs and uninfected controls, weekly during 35 days following the acute phase of infection, and immunoglobulin profiles were determined by ELISA. Dogs infected with BT exhibited unaltered levels of IgG2, increases in IgM, IgE, IgA, IgG and IgG1. In contrast, dogs infected with MT presented unaltered levels of IgE and IgG1 and an increase in IgM, IgA, IgG and IgG2 levels. Compared with the MT group, animals infected with BT showed significant increases in IgM on days 7, 14 and 28, in IgA on days 7, 14 and 21, in IgE on days 7 and 14, in IgG on days 14 and 28, and in IgG1 on days 7, 14 and 21. Parasitemia levels of the infected animals were measured over the same time period. No correlations were found between the immunoglobulin profiles and the parasi-temia levels. The results demonstrated that the inoculum source (BT or MT) influence the immunoglob-ulin isotype profile that may drive distinct outcome of acute canine Chagas disease |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2012-07-05T13:54:29Z |
dc.date.available.fl_str_mv |
2012-07-05T13:54:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VITAL, W. C. et al. Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes. Experimental Parasitology, v. 120, n. 3, p. 269-274, nov. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0014489408001963>. Acesso em: 05 jul. 2012 |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/979 |
dc.identifier.issn.none.fl_str_mv |
00144894 |
identifier_str_mv |
VITAL, W. C. et al. Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes. Experimental Parasitology, v. 120, n. 3, p. 269-274, nov. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0014489408001963>. Acesso em: 05 jul. 2012 00144894 |
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http://www.repositorio.ufop.br/handle/123456789/979 |
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eng |
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eng |
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