Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2005 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/handle/2011/6121 |
Resumo: | We have examined the prevalence of gene cagA and vacA alleles in 129 patients, 69 with gastritis and 60 with peptic ulcer diseases from North Brazil and their relation with histopathological data. vacA and cagA genotype were determined by polymerase chain reaction. Hematoxylin-eosin staining was used for histological diagnosis. 96.6% of the patients were colonized by Helicobacter pylori strains harboring single vacA genotype (nont-mixed infection). Among them, 11.8% had subtype s1a, 67.8% had subtype s1b, and 17% subtype s2. In regard to the middle region analysis, m1 alleles were found in 75.4% and m2 in 21.2% of patients. The cagA gene was detected in 78% patients infected with H. pylori and was associated with the s1-m1 vacA genotype. The H. pylori strains, vacA s1b m1/cagA-positive, were associated with increased risk of peptic ulcer disease and higher amounts of lymphocytic and neutrophilic infiltrates and the presence of intestinal metaplasia. These findings show that cagA and vacA genotyping may have clinical relevance in Brazil. |
id |
UFPA_25125a24e2ef32726620cbfc4b48f325 |
---|---|
oai_identifier_str |
oai:repositorio.ufpa.br:2011/6121 |
network_acronym_str |
UFPA |
network_name_str |
Repositório Institucional da UFPA |
repository_id_str |
2123 |
spelling |
2014-11-27T16:59:49Z2014-11-27T16:59:49Z2005-12MARTINS, Luisa Caricio et al. Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 8, p. 875-881, dez. 2005. Disponível em: <http://www.scielo.br/pdf/mioc/v100n8/v100n8a09.pdf>. Acesso em: 25 nov. 2014. <http://dx.doi.org/10.1590/S0074-02762005000800009>.0074-0276http://repositorio.ufpa.br/jspui/handle/2011/6121We have examined the prevalence of gene cagA and vacA alleles in 129 patients, 69 with gastritis and 60 with peptic ulcer diseases from North Brazil and their relation with histopathological data. vacA and cagA genotype were determined by polymerase chain reaction. Hematoxylin-eosin staining was used for histological diagnosis. 96.6% of the patients were colonized by Helicobacter pylori strains harboring single vacA genotype (nont-mixed infection). Among them, 11.8% had subtype s1a, 67.8% had subtype s1b, and 17% subtype s2. In regard to the middle region analysis, m1 alleles were found in 75.4% and m2 in 21.2% of patients. The cagA gene was detected in 78% patients infected with H. pylori and was associated with the s1-m1 vacA genotype. The H. pylori strains, vacA s1b m1/cagA-positive, were associated with increased risk of peptic ulcer disease and higher amounts of lymphocytic and neutrophilic infiltrates and the presence of intestinal metaplasia. These findings show that cagA and vacA genotyping may have clinical relevance in Brazil.Submitted by Edisangela Bastos (edisangela@ufpa.br) on 2014-11-26T22:26:51Z No. of bitstreams: 2 license_rdf: 22190 bytes, checksum: 19e8a2b57ef43c09f4d7071d2153c97d (MD5) Artigo_ClinicalPathologicalImportance.pdf: 437644 bytes, checksum: f664ab624a7db51f1bd42a44332a3f37 (MD5)Approved for entry into archive by Ana Rosa Silva (arosa@ufpa.br) on 2014-11-27T16:59:49Z (GMT) No. of bitstreams: 2 license_rdf: 22190 bytes, checksum: 19e8a2b57ef43c09f4d7071d2153c97d (MD5) Artigo_ClinicalPathologicalImportance.pdf: 437644 bytes, checksum: f664ab624a7db51f1bd42a44332a3f37 (MD5)Made available in DSpace on 2014-11-27T16:59:49Z (GMT). No. of bitstreams: 2 license_rdf: 22190 bytes, checksum: 19e8a2b57ef43c09f4d7071d2153c97d (MD5) Artigo_ClinicalPathologicalImportance.pdf: 437644 bytes, checksum: f664ab624a7db51f1bd42a44332a3f37 (MD5) Previous issue date: 2005-12engHelicobacter pyloriPrevalênciaÚlcera pépticaAlelos vacAEstatos cagABrasil - PaísClinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://lattes.cnpq.br/http://lattes.cnpq.br/7253864056606024MARTINS, Luisa CaricioCORVELO, Tereza Cristina de OliveiraDEMACHKI, SâmiaARAÚJO, Marialva Tereza Ferreira deASSUMPÇÃO, Mônica Baraúna deVILAR, Simone Cristina Araujo JucáFREITAS, Felipe BonfimBARBOSA, Hivana Patricia MeloFECURY, Amanda AlvesAMARAL, Renata Kelly Costa doSANTOS, Sidney Emanuel Batista dosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAORIGINALArtigo_ClinicalPathologicalImportance.pdfArtigo_ClinicalPathologicalImportance.pdfapplication/pdf437644https://repositorio.ufpa.br/oai/bitstream/2011/6121/1/Artigo_ClinicalPathologicalImportance.pdff664ab624a7db51f1bd42a44332a3f37MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849https://repositorio.ufpa.br/oai/bitstream/2011/6121/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-822302https://repositorio.ufpa.br/oai/bitstream/2011/6121/3/license_text23f63091ab682deaf673f6bd687eadb1MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-822190https://repositorio.ufpa.br/oai/bitstream/2011/6121/4/license_rdf19e8a2b57ef43c09f4d7071d2153c97dMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81704https://repositorio.ufpa.br/oai/bitstream/2011/6121/5/license.txt8d73ce52af4613a96b33573d09bea8c9MD55TEXTArtigo_ClinicalPathologicalImportance.pdf.txtArtigo_ClinicalPathologicalImportance.pdf.txtExtracted texttext/plain33289https://repositorio.ufpa.br/oai/bitstream/2011/6121/6/Artigo_ClinicalPathologicalImportance.pdf.txt4abb07256d7c83ad7c97f2c5ee3c4414MD562011/61212023-11-21 11:50:35.388oai:repositorio.ufpa.br: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ório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232023-11-21T14:50:35Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false |
dc.title.pt_BR.fl_str_mv |
Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil |
title |
Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil |
spellingShingle |
Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil MARTINS, Luisa Caricio Helicobacter pylori Prevalência Úlcera péptica Alelos vacA Estatos cagA Brasil - País |
title_short |
Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil |
title_full |
Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil |
title_fullStr |
Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil |
title_full_unstemmed |
Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil |
title_sort |
Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil |
author |
MARTINS, Luisa Caricio |
author_facet |
MARTINS, Luisa Caricio CORVELO, Tereza Cristina de Oliveira DEMACHKI, Sâmia ARAÚJO, Marialva Tereza Ferreira de ASSUMPÇÃO, Mônica Baraúna de VILAR, Simone Cristina Araujo Jucá FREITAS, Felipe Bonfim BARBOSA, Hivana Patricia Melo FECURY, Amanda Alves AMARAL, Renata Kelly Costa do SANTOS, Sidney Emanuel Batista dos |
author_role |
author |
author2 |
CORVELO, Tereza Cristina de Oliveira DEMACHKI, Sâmia ARAÚJO, Marialva Tereza Ferreira de ASSUMPÇÃO, Mônica Baraúna de VILAR, Simone Cristina Araujo Jucá FREITAS, Felipe Bonfim BARBOSA, Hivana Patricia Melo FECURY, Amanda Alves AMARAL, Renata Kelly Costa do SANTOS, Sidney Emanuel Batista dos |
author2_role |
author author author author author author author author author author |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/ http://lattes.cnpq.br/7253864056606024 |
dc.contributor.author.fl_str_mv |
MARTINS, Luisa Caricio CORVELO, Tereza Cristina de Oliveira DEMACHKI, Sâmia ARAÚJO, Marialva Tereza Ferreira de ASSUMPÇÃO, Mônica Baraúna de VILAR, Simone Cristina Araujo Jucá FREITAS, Felipe Bonfim BARBOSA, Hivana Patricia Melo FECURY, Amanda Alves AMARAL, Renata Kelly Costa do SANTOS, Sidney Emanuel Batista dos |
dc.subject.por.fl_str_mv |
Helicobacter pylori Prevalência Úlcera péptica Alelos vacA Estatos cagA Brasil - País |
topic |
Helicobacter pylori Prevalência Úlcera péptica Alelos vacA Estatos cagA Brasil - País |
description |
We have examined the prevalence of gene cagA and vacA alleles in 129 patients, 69 with gastritis and 60 with peptic ulcer diseases from North Brazil and their relation with histopathological data. vacA and cagA genotype were determined by polymerase chain reaction. Hematoxylin-eosin staining was used for histological diagnosis. 96.6% of the patients were colonized by Helicobacter pylori strains harboring single vacA genotype (nont-mixed infection). Among them, 11.8% had subtype s1a, 67.8% had subtype s1b, and 17% subtype s2. In regard to the middle region analysis, m1 alleles were found in 75.4% and m2 in 21.2% of patients. The cagA gene was detected in 78% patients infected with H. pylori and was associated with the s1-m1 vacA genotype. The H. pylori strains, vacA s1b m1/cagA-positive, were associated with increased risk of peptic ulcer disease and higher amounts of lymphocytic and neutrophilic infiltrates and the presence of intestinal metaplasia. These findings show that cagA and vacA genotyping may have clinical relevance in Brazil. |
publishDate |
2005 |
dc.date.issued.fl_str_mv |
2005-12 |
dc.date.accessioned.fl_str_mv |
2014-11-27T16:59:49Z |
dc.date.available.fl_str_mv |
2014-11-27T16:59:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARTINS, Luisa Caricio et al. Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 8, p. 875-881, dez. 2005. Disponível em: <http://www.scielo.br/pdf/mioc/v100n8/v100n8a09.pdf>. Acesso em: 25 nov. 2014. <http://dx.doi.org/10.1590/S0074-02762005000800009>. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/handle/2011/6121 |
dc.identifier.issn.none.fl_str_mv |
0074-0276 |
identifier_str_mv |
MARTINS, Luisa Caricio et al. Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 8, p. 875-881, dez. 2005. Disponível em: <http://www.scielo.br/pdf/mioc/v100n8/v100n8a09.pdf>. Acesso em: 25 nov. 2014. <http://dx.doi.org/10.1590/S0074-02762005000800009>. 0074-0276 |
url |
http://repositorio.ufpa.br/jspui/handle/2011/6121 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFPA instname:Universidade Federal do Pará (UFPA) instacron:UFPA |
instname_str |
Universidade Federal do Pará (UFPA) |
instacron_str |
UFPA |
institution |
UFPA |
reponame_str |
Repositório Institucional da UFPA |
collection |
Repositório Institucional da UFPA |
bitstream.url.fl_str_mv |
https://repositorio.ufpa.br/oai/bitstream/2011/6121/1/Artigo_ClinicalPathologicalImportance.pdf https://repositorio.ufpa.br/oai/bitstream/2011/6121/2/license_url https://repositorio.ufpa.br/oai/bitstream/2011/6121/3/license_text https://repositorio.ufpa.br/oai/bitstream/2011/6121/4/license_rdf https://repositorio.ufpa.br/oai/bitstream/2011/6121/5/license.txt https://repositorio.ufpa.br/oai/bitstream/2011/6121/6/Artigo_ClinicalPathologicalImportance.pdf.txt |
bitstream.checksum.fl_str_mv |
f664ab624a7db51f1bd42a44332a3f37 4afdbb8c545fd630ea7db775da747b2f 23f63091ab682deaf673f6bd687eadb1 19e8a2b57ef43c09f4d7071d2153c97d 8d73ce52af4613a96b33573d09bea8c9 4abb07256d7c83ad7c97f2c5ee3c4414 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA) |
repository.mail.fl_str_mv |
riufpabc@ufpa.br |
_version_ |
1797787881623781376 |