Estudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantes
Main Author: | |
---|---|
Publication Date: | 2011 |
Format: | Doctoral thesis |
Language: | por |
Source: | Repositório Institucional da UFPel - Guaiaca |
Download full: | http://repositorio.ufpel.edu.br/handle/ri/2561 |
Summary: | Sporotrichosis has been receiving attention in any world in the several areas of the health as an important fungal disease. So, this work aimed: (i) to study cases of sporotrichosis diagnosed in the south region of Rio Grande do Sul in a period of ten years, (ii) to study the morphological and pathogenicity characteristics of S. schenckii isolates, (iii) to determine the sensitivity of S. schenckii isolates to the commercial disinfectants. Sporotrichosis cases were analysed as for geographical distribution, it clinical forms of the disease, presence of the fungus in different anatomical sites and tecidual inflammatory response. The 42 S. schenckii isolates originating from clinical cases, environment isolates and reference strains used in the phenotypic study were evaluated as for macromorphology in Sabouraud agar added of chloramphenicol, potato agar and ágar lactrimel, incubated 25ºC and 35ºC for 21 days. The micromorphology was analyzed in potato agar to 25ºC whereas the termotholerance was evaluated in ágar Sabouraud added of 1% yeast extract, incubated 41ºC for seven days. Ultraestructural study of nine clinical isolates and two reference strains of S. schenckii were carried by transmission electron microscopy. Susceptibility of 12 S. schenckii isolates to disinfectants was performed by the techniques of broth microdilution, agar diffusion and direct exposute test. The 103 cases of sporotrichosis in animals demonstrated that this is an endemic mycosis in the south region of Rio Grande do Sul being more frequent in young felines and adult dogs. The felines developed disseminated cutaneous form with more frequency. Therefore, in canines, the fixed cutaneous form was predominant though it has taken place in 18.2% of the dog to respiratory form with absence of cutaneous lesions. Research of the S. schenckii in nails, oral cavity, conjunctival mucosa, blood and testicles demonstrated high fungal positivity in samples of oral cavity (45.2%) and testicles (46.6%). The study of the hematological alterations related with the clinical form of the disease demonstrated that animals with the cutaneous fixed form of the mycosis do not present alterations in the blood count. However, animals with the cutaneous disseminated form develop important alterations characterized mainly for leucocytosis with neutrophilia and anaemia. Morphological analyzes of 42 clinical and environmental isolates of S. schenckii demonstrated macro and micromorphogical differences in the different culture medium utilized. In the thermotolerance evaluation 26.2% isolates were capable of growth at 41oC, specially isolates of feline sporotrichosis. The ultraestructural study showed presence of granules of melanin in all S. schenckii isolates of canine sporotrichosis and correlation among melanin and cell wall thickness. In vitro evaluation of disinfectants demonstrated chlorexidine digluconate antifungal activity in the microdilution broth and agar diffusion techniques and the sodium hypochlorite showed best performance in the direct exposure test. The presence of the organic matter interfered in the antifungal action of both disinfectants. Sodium hypochlorite showed better results with total elimination of the fungal load in the absence of organic matter and a reduction of 60 % of the fungal load in the presence of this. |
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http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4732365D1http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799478H9Nobre, Márcia de Oliveirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705228J5Fernandes, Cristina Gevehrhttp://buscatextual.cnpq.br/buscatextual/index.jspNascente, Patrícia da Silvahttp://lattes.cnpq.br/3560823637748071Xavier, Melissa Orzechowskihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776164Z7Meireles, Mario Carlos AraújoMadrid, Isabel Martins2014-08-20T14:38:01Z2011-05-232014-08-20T14:38:01Z2011-02-25MADRID, Isabel Martins. Estudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantes. 2011. 113 f. Tese (Doutorado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2011.http://repositorio.ufpel.edu.br/handle/ri/2561Sporotrichosis has been receiving attention in any world in the several areas of the health as an important fungal disease. So, this work aimed: (i) to study cases of sporotrichosis diagnosed in the south region of Rio Grande do Sul in a period of ten years, (ii) to study the morphological and pathogenicity characteristics of S. schenckii isolates, (iii) to determine the sensitivity of S. schenckii isolates to the commercial disinfectants. Sporotrichosis cases were analysed as for geographical distribution, it clinical forms of the disease, presence of the fungus in different anatomical sites and tecidual inflammatory response. The 42 S. schenckii isolates originating from clinical cases, environment isolates and reference strains used in the phenotypic study were evaluated as for macromorphology in Sabouraud agar added of chloramphenicol, potato agar and ágar lactrimel, incubated 25ºC and 35ºC for 21 days. The micromorphology was analyzed in potato agar to 25ºC whereas the termotholerance was evaluated in ágar Sabouraud added of 1% yeast extract, incubated 41ºC for seven days. Ultraestructural study of nine clinical isolates and two reference strains of S. schenckii were carried by transmission electron microscopy. Susceptibility of 12 S. schenckii isolates to disinfectants was performed by the techniques of broth microdilution, agar diffusion and direct exposute test. The 103 cases of sporotrichosis in animals demonstrated that this is an endemic mycosis in the south region of Rio Grande do Sul being more frequent in young felines and adult dogs. The felines developed disseminated cutaneous form with more frequency. Therefore, in canines, the fixed cutaneous form was predominant though it has taken place in 18.2% of the dog to respiratory form with absence of cutaneous lesions. Research of the S. schenckii in nails, oral cavity, conjunctival mucosa, blood and testicles demonstrated high fungal positivity in samples of oral cavity (45.2%) and testicles (46.6%). The study of the hematological alterations related with the clinical form of the disease demonstrated that animals with the cutaneous fixed form of the mycosis do not present alterations in the blood count. However, animals with the cutaneous disseminated form develop important alterations characterized mainly for leucocytosis with neutrophilia and anaemia. Morphological analyzes of 42 clinical and environmental isolates of S. schenckii demonstrated macro and micromorphogical differences in the different culture medium utilized. In the thermotolerance evaluation 26.2% isolates were capable of growth at 41oC, specially isolates of feline sporotrichosis. The ultraestructural study showed presence of granules of melanin in all S. schenckii isolates of canine sporotrichosis and correlation among melanin and cell wall thickness. In vitro evaluation of disinfectants demonstrated chlorexidine digluconate antifungal activity in the microdilution broth and agar diffusion techniques and the sodium hypochlorite showed best performance in the direct exposure test. The presence of the organic matter interfered in the antifungal action of both disinfectants. Sodium hypochlorite showed better results with total elimination of the fungal load in the absence of organic matter and a reduction of 60 % of the fungal load in the presence of this.A esporotricose tem se destacado mundialmente nas diversas áreas da saúde como uma importante doença fúngica. Assim, este trabalho teve como objetivos: (i) estudar casos de esporotricose diagnosticados na região sul do Rio Grande do Sul num período de dez anos, (ii) estudar as características morfológicas e de patogenicidade de isolados de Sporothrix schenckii, (iii) determinar a suscetibilidade de S. schenckii a desinfetantes comerciais. Os casos de esporotricose foram analisados quanto a distribuição geográfica, forma clíinica da doença, presença do fungo em diferentes sítios anatômicos e resposta tecidual. Os 42 isolados de S. schenckii provenientes de casos clínicos, isolados do ambiente e cepas de referência utilizados no estudo fenotípico foram avaliados quanto a macromorfologia em ágar Sabouraud acrescido de cloranfenicol, ágar batata e ágar lactrimel incubados a 25ºC e 35ºC por 21 dias. A micromorfologia foi avaliada em ágar batata a 25ºC enquanto que a termotolerância foi avaliada em ágar Sabouraud acrecido de extrato de levedura 1% incubado a 41ºC por sete dias. O estudo ultraestrural dos nove isolados clínicos e duas cepas de referência de S. schenckii foi realizado por microscopia eletrônica de transmissão. A suscetiblidade de 12 isolados de S. schenckii a desinfetantes foi realizada pelas técnicas de microdiluição em caldo, difusão em agar e teste de exposição direta. Os 103 casos de esporotricose em animais demonstraram que esta é uma micose endêmica na região sul do Rio Grande do Sul ocorrendo com mais freqüência em felinos jovens e cães adultos. Os felinos desenvolveram principalmente a forma cutânea disseminada enquanto que em caninos, a forma cutânea fixa foi a mais freqüente embora tenha ocorrido em 18,2% dos caninos, a forma respiratória com ausência de lesões cutâneas. A pesquisa do fungo S. schenckii em unhas, cavidade oral, mucosa conjuntival, sangue e testículos revelou alta positividade fúngica em amostras de testículos (46,6%) e cavidade oral (45,2%). O hemograma de animais com a forma cutânea fixa não apresentou alterações nos valores hematológicos enquanto que aqueles com a forma cutânea disseminada apresentaram alterações tanto no leucograma como no eritrograma, caracterizadas por leucocitose por neutrofilia e anemia normocítica e normocrômica. A análise morfológica de 42 isolados clínicos e do ambiente de S. schenckii demonstrou diferenças macro e micromorfológicas nos diferentes meios de cultivo utilizados. Na avaliação da termotolerância obteve-se 26,2% dos isolados termotolerantes, sendo a maioria proveniente de casos de esporotricose felina. O estudo ultraestrutural revelou presença de grânulos de melanina em todos os isolados de S. schenckii originados de esporotricose canina e correlação entre presença de melanina e espessura da parede celular. A avaliação in vitro de desinfetantes demonstrou maior ação antifúngica do digluconato de clorexidina nas técnicas de microdiluição em caldo e difusão em agar enquanto que o hipoclorido de sódio apresentou melhor eficácia na técnica de exposição direta. A presença de matéria orgânica interferiu na ação antifúngica de ambos os desinfetantes, entretanto, o hipoclorito de sódio apresentou melhores resultados com inativação total da carga fúngica na ausência de matéria orgânica e redução de 60% da carga fúngica na presença desta.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em VeterináriaUFPelBRVeterináriaVeterináriaEsporotricosePatogenicidadeMorfologiaAtividade antifúngicaTestes de suscetibilidadeVeterinarySporotrichosisPathogenicityMorphologyAntifungal activitySusceptibility testsCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMALEstudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantesStufy of the phenotypic characteristics, pathogenicity factors and susceptibility of the Sporothrix schenckii isolates against disinfectantsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALtese_isabel_madrid.pdfapplication/pdf2158071http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2561/1/tese_isabel_madrid.pdf0cdf6bd60773cb623d04e6df539e8aa9MD51open accessTEXTtese_isabel_madrid.pdf.txttese_isabel_madrid.pdf.txtExtracted Texttext/plain180481http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2561/2/tese_isabel_madrid.pdf.txt978fb89a6f5952e99cf9b57c731608bbMD52open accessTHUMBNAILtese_isabel_madrid.pdf.jpgtese_isabel_madrid.pdf.jpgGenerated Thumbnailimage/jpeg1692http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2561/3/tese_isabel_madrid.pdf.jpg979855a3f9d284318e857aaa568d7317MD53open access123456789/25612022-01-27 09:35:19.978open accessoai:guaiaca.ufpel.edu.br:123456789/2561Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2022-01-27T12:35:19Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Estudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantes |
dc.title.alternative.eng.fl_str_mv |
Stufy of the phenotypic characteristics, pathogenicity factors and susceptibility of the Sporothrix schenckii isolates against disinfectants |
title |
Estudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantes |
spellingShingle |
Estudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantes Madrid, Isabel Martins Veterinária Esporotricose Patogenicidade Morfologia Atividade antifúngica Testes de suscetibilidade Veterinary Sporotrichosis Pathogenicity Morphology Antifungal activity Susceptibility tests CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMAL |
title_short |
Estudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantes |
title_full |
Estudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantes |
title_fullStr |
Estudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantes |
title_full_unstemmed |
Estudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantes |
title_sort |
Estudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantes |
author |
Madrid, Isabel Martins |
author_facet |
Madrid, Isabel Martins |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4732365D1 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799478H9 |
dc.contributor.referees1.pt_BR.fl_str_mv |
Nascente, Patrícia da Silva |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://lattes.cnpq.br/3560823637748071 |
dc.contributor.referees2.pt_BR.fl_str_mv |
Xavier, Melissa Orzechowski |
dc.contributor.referees2ID.por.fl_str_mv |
|
dc.contributor.referees2Lattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776164Z7 |
dc.contributor.advisor-co1.fl_str_mv |
Nobre, Márcia de Oliveira |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705228J5 |
dc.contributor.advisor-co2.fl_str_mv |
Fernandes, Cristina Gevehr |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/index.jsp |
dc.contributor.advisor1.fl_str_mv |
Meireles, Mario Carlos Araújo |
dc.contributor.author.fl_str_mv |
Madrid, Isabel Martins |
contributor_str_mv |
Nobre, Márcia de Oliveira Fernandes, Cristina Gevehr Meireles, Mario Carlos Araújo |
dc.subject.por.fl_str_mv |
Veterinária Esporotricose Patogenicidade Morfologia Atividade antifúngica Testes de suscetibilidade |
topic |
Veterinária Esporotricose Patogenicidade Morfologia Atividade antifúngica Testes de suscetibilidade Veterinary Sporotrichosis Pathogenicity Morphology Antifungal activity Susceptibility tests CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMAL |
dc.subject.eng.fl_str_mv |
Veterinary Sporotrichosis Pathogenicity Morphology Antifungal activity Susceptibility tests |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMAL |
description |
Sporotrichosis has been receiving attention in any world in the several areas of the health as an important fungal disease. So, this work aimed: (i) to study cases of sporotrichosis diagnosed in the south region of Rio Grande do Sul in a period of ten years, (ii) to study the morphological and pathogenicity characteristics of S. schenckii isolates, (iii) to determine the sensitivity of S. schenckii isolates to the commercial disinfectants. Sporotrichosis cases were analysed as for geographical distribution, it clinical forms of the disease, presence of the fungus in different anatomical sites and tecidual inflammatory response. The 42 S. schenckii isolates originating from clinical cases, environment isolates and reference strains used in the phenotypic study were evaluated as for macromorphology in Sabouraud agar added of chloramphenicol, potato agar and ágar lactrimel, incubated 25ºC and 35ºC for 21 days. The micromorphology was analyzed in potato agar to 25ºC whereas the termotholerance was evaluated in ágar Sabouraud added of 1% yeast extract, incubated 41ºC for seven days. Ultraestructural study of nine clinical isolates and two reference strains of S. schenckii were carried by transmission electron microscopy. Susceptibility of 12 S. schenckii isolates to disinfectants was performed by the techniques of broth microdilution, agar diffusion and direct exposute test. The 103 cases of sporotrichosis in animals demonstrated that this is an endemic mycosis in the south region of Rio Grande do Sul being more frequent in young felines and adult dogs. The felines developed disseminated cutaneous form with more frequency. Therefore, in canines, the fixed cutaneous form was predominant though it has taken place in 18.2% of the dog to respiratory form with absence of cutaneous lesions. Research of the S. schenckii in nails, oral cavity, conjunctival mucosa, blood and testicles demonstrated high fungal positivity in samples of oral cavity (45.2%) and testicles (46.6%). The study of the hematological alterations related with the clinical form of the disease demonstrated that animals with the cutaneous fixed form of the mycosis do not present alterations in the blood count. However, animals with the cutaneous disseminated form develop important alterations characterized mainly for leucocytosis with neutrophilia and anaemia. Morphological analyzes of 42 clinical and environmental isolates of S. schenckii demonstrated macro and micromorphogical differences in the different culture medium utilized. In the thermotolerance evaluation 26.2% isolates were capable of growth at 41oC, specially isolates of feline sporotrichosis. The ultraestructural study showed presence of granules of melanin in all S. schenckii isolates of canine sporotrichosis and correlation among melanin and cell wall thickness. In vitro evaluation of disinfectants demonstrated chlorexidine digluconate antifungal activity in the microdilution broth and agar diffusion techniques and the sodium hypochlorite showed best performance in the direct exposure test. The presence of the organic matter interfered in the antifungal action of both disinfectants. Sodium hypochlorite showed better results with total elimination of the fungal load in the absence of organic matter and a reduction of 60 % of the fungal load in the presence of this. |
publishDate |
2011 |
dc.date.available.fl_str_mv |
2011-05-23 2014-08-20T14:38:01Z |
dc.date.issued.fl_str_mv |
2011-02-25 |
dc.date.accessioned.fl_str_mv |
2014-08-20T14:38:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MADRID, Isabel Martins. Estudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantes. 2011. 113 f. Tese (Doutorado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2561 |
identifier_str_mv |
MADRID, Isabel Martins. Estudo das características fenotípicas, fatores de patogenicidade e suscetibilidade de isolados de Sporothrix schenckii frente a desinfetantes. 2011. 113 f. Tese (Doutorado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2011. |
url |
http://repositorio.ufpel.edu.br/handle/ri/2561 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Veterinária |
dc.publisher.initials.fl_str_mv |
UFPel |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
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