Molecular Identification of Burkholderia Cepacia Complex and Species Distribution Among Cystic Fibrosis Patients Seen at the Reference Center in Southern Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Clinical and Biomedical Research |
Texto Completo: | https://seer.ufrgs.br/index.php/hcpa/article/view/21153 |
Resumo: | Background: Burkholderia cepacia complex (Bcc) infections in cystic fibrosis (CF) patients are associated with decline in lung function and reduced survival. The potential transmissibility of Bcc among CF patients has been reported, indicating that strict segregation of CF patients with Bcc is crucial.Aims: To standardize the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) assay in order to identify Bcc species and to establish the prevalence of Bcc species and their susceptibility profile among CF patients seen at the Hospital de Clínicas de Porto Alegre (HCPA).Methods: The classification of the clinical isolates recovered from respiratory tract specimens of CF patients as Bcc was achieved using the API-20NE® phenotypic commercial system. The identification of the Bcc species was performed using PCR-RFLP. The antimicrobial disk diffusion susceptibility testing was performed according to the CLSI (2006).Results: API-20NE® was able to identify Bcc isolates (244 specimens), such as B. cepacia, indicating that it was not able to distinguish among the Bcc species. The PCR-RFLP molecular method discriminated the eight reference Bcc species, thus validating the method for clinical isolates. Bcc prevalence determined by PCR-RFLP was 10.6% (26/244). The molecular analysis identified B. cenocepacia in 53.8% (14/26) of infected patients, B. multivorans in 15.4% (4/26), and B. vietnamiensis and B. ambifaria in 7.7% (2/26). The antibiotic resistance profile was variable among Bcc species.Conclusions: The PCR-RFLP method was validated for the identification of Bcc species. B. cenocepacia proved to be the most prevalent species among the CF patients seen at the HCPA. |
id |
UFRGS-20_4f8de24168079a4b88bea9813fcfc0e3 |
---|---|
oai_identifier_str |
oai:seer.ufrgs.br:article/21153 |
network_acronym_str |
UFRGS-20 |
network_name_str |
Clinical and Biomedical Research |
repository_id_str |
|
spelling |
Molecular Identification of Burkholderia Cepacia Complex and Species Distribution Among Cystic Fibrosis Patients Seen at the Reference Center in Southern BrazilBurkholderia cepaciafibrose císticaPCR-RFLPgene recABurkholderia cepaciacystic fibrosisPCR-RFLPrecA geneBackground: Burkholderia cepacia complex (Bcc) infections in cystic fibrosis (CF) patients are associated with decline in lung function and reduced survival. The potential transmissibility of Bcc among CF patients has been reported, indicating that strict segregation of CF patients with Bcc is crucial.Aims: To standardize the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) assay in order to identify Bcc species and to establish the prevalence of Bcc species and their susceptibility profile among CF patients seen at the Hospital de Clínicas de Porto Alegre (HCPA).Methods: The classification of the clinical isolates recovered from respiratory tract specimens of CF patients as Bcc was achieved using the API-20NE® phenotypic commercial system. The identification of the Bcc species was performed using PCR-RFLP. The antimicrobial disk diffusion susceptibility testing was performed according to the CLSI (2006).Results: API-20NE® was able to identify Bcc isolates (244 specimens), such as B. cepacia, indicating that it was not able to distinguish among the Bcc species. The PCR-RFLP molecular method discriminated the eight reference Bcc species, thus validating the method for clinical isolates. Bcc prevalence determined by PCR-RFLP was 10.6% (26/244). The molecular analysis identified B. cenocepacia in 53.8% (14/26) of infected patients, B. multivorans in 15.4% (4/26), and B. vietnamiensis and B. ambifaria in 7.7% (2/26). The antibiotic resistance profile was variable among Bcc species.Conclusions: The PCR-RFLP method was validated for the identification of Bcc species. B. cenocepacia proved to be the most prevalent species among the CF patients seen at the HCPA.HCPA/FAMED/UFRGS2011-07-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed ArticleAvaliado por Paresapplication/pdfhttps://seer.ufrgs.br/index.php/hcpa/article/view/21153Clinical & Biomedical Research; Vol. 31 No. 2 (2011): Especial Fibrose Cística: Revista HCPAClinical and Biomedical Research; v. 31 n. 2 (2011): Especial Fibrose Cística2357-9730reponame:Clinical and Biomedical Researchinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSporhttps://seer.ufrgs.br/index.php/hcpa/article/view/21153/12735Leite, Fernanda ConcliPinheiro Machado, Alice Beatriz MombachLutz, LarissaVieira, Maria IzoleteBarth, Afonso Luisinfo:eu-repo/semantics/openAccess2020-01-16T18:02:07Zoai:seer.ufrgs.br:article/21153Revistahttps://www.seer.ufrgs.br/index.php/hcpaPUBhttps://seer.ufrgs.br/index.php/hcpa/oai||cbr@hcpa.edu.br2357-97302357-9730opendoar:2020-01-16T18:02:07Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.none.fl_str_mv |
Molecular Identification of Burkholderia Cepacia Complex and Species Distribution Among Cystic Fibrosis Patients Seen at the Reference Center in Southern Brazil |
title |
Molecular Identification of Burkholderia Cepacia Complex and Species Distribution Among Cystic Fibrosis Patients Seen at the Reference Center in Southern Brazil |
spellingShingle |
Molecular Identification of Burkholderia Cepacia Complex and Species Distribution Among Cystic Fibrosis Patients Seen at the Reference Center in Southern Brazil Leite, Fernanda Concli Burkholderia cepacia fibrose cística PCR-RFLP gene recA Burkholderia cepacia cystic fibrosis PCR-RFLP recA gene |
title_short |
Molecular Identification of Burkholderia Cepacia Complex and Species Distribution Among Cystic Fibrosis Patients Seen at the Reference Center in Southern Brazil |
title_full |
Molecular Identification of Burkholderia Cepacia Complex and Species Distribution Among Cystic Fibrosis Patients Seen at the Reference Center in Southern Brazil |
title_fullStr |
Molecular Identification of Burkholderia Cepacia Complex and Species Distribution Among Cystic Fibrosis Patients Seen at the Reference Center in Southern Brazil |
title_full_unstemmed |
Molecular Identification of Burkholderia Cepacia Complex and Species Distribution Among Cystic Fibrosis Patients Seen at the Reference Center in Southern Brazil |
title_sort |
Molecular Identification of Burkholderia Cepacia Complex and Species Distribution Among Cystic Fibrosis Patients Seen at the Reference Center in Southern Brazil |
author |
Leite, Fernanda Concli |
author_facet |
Leite, Fernanda Concli Pinheiro Machado, Alice Beatriz Mombach Lutz, Larissa Vieira, Maria Izolete Barth, Afonso Luis |
author_role |
author |
author2 |
Pinheiro Machado, Alice Beatriz Mombach Lutz, Larissa Vieira, Maria Izolete Barth, Afonso Luis |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Leite, Fernanda Concli Pinheiro Machado, Alice Beatriz Mombach Lutz, Larissa Vieira, Maria Izolete Barth, Afonso Luis |
dc.subject.por.fl_str_mv |
Burkholderia cepacia fibrose cística PCR-RFLP gene recA Burkholderia cepacia cystic fibrosis PCR-RFLP recA gene |
topic |
Burkholderia cepacia fibrose cística PCR-RFLP gene recA Burkholderia cepacia cystic fibrosis PCR-RFLP recA gene |
description |
Background: Burkholderia cepacia complex (Bcc) infections in cystic fibrosis (CF) patients are associated with decline in lung function and reduced survival. The potential transmissibility of Bcc among CF patients has been reported, indicating that strict segregation of CF patients with Bcc is crucial.Aims: To standardize the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) assay in order to identify Bcc species and to establish the prevalence of Bcc species and their susceptibility profile among CF patients seen at the Hospital de Clínicas de Porto Alegre (HCPA).Methods: The classification of the clinical isolates recovered from respiratory tract specimens of CF patients as Bcc was achieved using the API-20NE® phenotypic commercial system. The identification of the Bcc species was performed using PCR-RFLP. The antimicrobial disk diffusion susceptibility testing was performed according to the CLSI (2006).Results: API-20NE® was able to identify Bcc isolates (244 specimens), such as B. cepacia, indicating that it was not able to distinguish among the Bcc species. The PCR-RFLP molecular method discriminated the eight reference Bcc species, thus validating the method for clinical isolates. Bcc prevalence determined by PCR-RFLP was 10.6% (26/244). The molecular analysis identified B. cenocepacia in 53.8% (14/26) of infected patients, B. multivorans in 15.4% (4/26), and B. vietnamiensis and B. ambifaria in 7.7% (2/26). The antibiotic resistance profile was variable among Bcc species.Conclusions: The PCR-RFLP method was validated for the identification of Bcc species. B. cenocepacia proved to be the most prevalent species among the CF patients seen at the HCPA. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-07-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Avaliado por Pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufrgs.br/index.php/hcpa/article/view/21153 |
url |
https://seer.ufrgs.br/index.php/hcpa/article/view/21153 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.ufrgs.br/index.php/hcpa/article/view/21153/12735 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
HCPA/FAMED/UFRGS |
publisher.none.fl_str_mv |
HCPA/FAMED/UFRGS |
dc.source.none.fl_str_mv |
Clinical & Biomedical Research; Vol. 31 No. 2 (2011): Especial Fibrose Cística: Revista HCPA Clinical and Biomedical Research; v. 31 n. 2 (2011): Especial Fibrose Cística 2357-9730 reponame:Clinical and Biomedical Research instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Clinical and Biomedical Research |
collection |
Clinical and Biomedical Research |
repository.name.fl_str_mv |
Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
||cbr@hcpa.edu.br |
_version_ |
1750135156078804992 |