Caffeine teratogenicity in rats: morphological characterization and hypothesized mechanisms

Detalhes bibliográficos
Autor(a) principal: Souza, Ana Claudia
Data de Publicação: 2017
Outros Autores: Dussán-Sarria, Jairo Alberto, Souza, Andressa, Caumo, Wolnei, Torres, Iraci Lucena da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinical and Biomedical Research
Texto Completo: https://seer.ufrgs.br/index.php/hcpa/article/view/68287
Resumo: Caffeine consumption during pregnancy has been shown in the scientific literature to be associated with teratogenicity such as low birth weight, fetal malformations, and miscarriage. However, the morphological alterations of the offspring of dams exposed during pregnancy have not been consistently described, and the mechanisms why they occur remain elusive. Thus, we aimed to characterize the offspring malformations induced by moderate and high doses of caffeine during pregnancy. Dams were divided into three groups: control, moderate (0.3 g/L), and high dose (1.0 g/L) of caffeine, which was provided in the drinking water beginning on gestational day 1 and continuing throughout the entire gestation. At moderate doses, only one of the dams had stillborn pups, although no macroscopic malformations were observed. High doses of caffeine induced significantly more malformations (P<0.001) and early death (before P4). The malformations observed were related to fetal development and cardiovascular alterations, namely bruises, macrocephaly with short limbs, abnormal development (or absence) of head structures and limbs, labial malformations, hydrops fetalis, and poor placental formation. We discussed the proposed mechanisms by which caffeine might induce these phenotypes, which may involve down-regulation of adenosine A1 receptors, and increased mothers’ catecholamines. Our findings further confirm the evidence of the teratogenic effects of high doses of caffeine administered during pregnancy. These findings support the recommendation to avoid caffeine exposure during pregnancy. Keywords: Caffeine; offspring; teratogenicity; pregnancy; hydrops; cardiovascular alterations
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spelling Caffeine teratogenicity in rats: morphological characterization and hypothesized mechanismsCaffeineoffspringteratogenicitypregnancyhydropscardiovascular alterationsTeratogenicityCaffeine consumption during pregnancy has been shown in the scientific literature to be associated with teratogenicity such as low birth weight, fetal malformations, and miscarriage. However, the morphological alterations of the offspring of dams exposed during pregnancy have not been consistently described, and the mechanisms why they occur remain elusive. Thus, we aimed to characterize the offspring malformations induced by moderate and high doses of caffeine during pregnancy. Dams were divided into three groups: control, moderate (0.3 g/L), and high dose (1.0 g/L) of caffeine, which was provided in the drinking water beginning on gestational day 1 and continuing throughout the entire gestation. At moderate doses, only one of the dams had stillborn pups, although no macroscopic malformations were observed. High doses of caffeine induced significantly more malformations (P<0.001) and early death (before P4). The malformations observed were related to fetal development and cardiovascular alterations, namely bruises, macrocephaly with short limbs, abnormal development (or absence) of head structures and limbs, labial malformations, hydrops fetalis, and poor placental formation. We discussed the proposed mechanisms by which caffeine might induce these phenotypes, which may involve down-regulation of adenosine A1 receptors, and increased mothers’ catecholamines. Our findings further confirm the evidence of the teratogenic effects of high doses of caffeine administered during pregnancy. These findings support the recommendation to avoid caffeine exposure during pregnancy. Keywords: Caffeine; offspring; teratogenicity; pregnancy; hydrops; cardiovascular alterationsHCPA/FAMED/UFRGS2017-01-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed ArticleAvaliado por Paresapplication/pdfhttps://seer.ufrgs.br/index.php/hcpa/article/view/68287Clinical & Biomedical Research; Vol. 36 No. 4 (2016)Clinical and Biomedical Research; v. 36 n. 4 (2016)2357-9730reponame:Clinical and Biomedical Researchinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSenghttps://seer.ufrgs.br/index.php/hcpa/article/view/68287/pdfCopyright (c) 2017 Clinical and Biomedical Researchinfo:eu-repo/semantics/openAccessSouza, Ana ClaudiaDussán-Sarria, Jairo AlbertoSouza, AndressaCaumo, WolneiTorres, Iraci Lucena da Silva2018-09-13T13:01:58Zoai:seer.ufrgs.br:article/68287Revistahttps://www.seer.ufrgs.br/index.php/hcpaPUBhttps://seer.ufrgs.br/index.php/hcpa/oai||cbr@hcpa.edu.br2357-97302357-9730opendoar:2018-09-13T13:01:58Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.none.fl_str_mv Caffeine teratogenicity in rats: morphological characterization and hypothesized mechanisms
title Caffeine teratogenicity in rats: morphological characterization and hypothesized mechanisms
spellingShingle Caffeine teratogenicity in rats: morphological characterization and hypothesized mechanisms
Souza, Ana Claudia
Caffeine
offspring
teratogenicity
pregnancy
hydrops
cardiovascular alterations
Teratogenicity
title_short Caffeine teratogenicity in rats: morphological characterization and hypothesized mechanisms
title_full Caffeine teratogenicity in rats: morphological characterization and hypothesized mechanisms
title_fullStr Caffeine teratogenicity in rats: morphological characterization and hypothesized mechanisms
title_full_unstemmed Caffeine teratogenicity in rats: morphological characterization and hypothesized mechanisms
title_sort Caffeine teratogenicity in rats: morphological characterization and hypothesized mechanisms
author Souza, Ana Claudia
author_facet Souza, Ana Claudia
Dussán-Sarria, Jairo Alberto
Souza, Andressa
Caumo, Wolnei
Torres, Iraci Lucena da Silva
author_role author
author2 Dussán-Sarria, Jairo Alberto
Souza, Andressa
Caumo, Wolnei
Torres, Iraci Lucena da Silva
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Souza, Ana Claudia
Dussán-Sarria, Jairo Alberto
Souza, Andressa
Caumo, Wolnei
Torres, Iraci Lucena da Silva
dc.subject.por.fl_str_mv Caffeine
offspring
teratogenicity
pregnancy
hydrops
cardiovascular alterations
Teratogenicity
topic Caffeine
offspring
teratogenicity
pregnancy
hydrops
cardiovascular alterations
Teratogenicity
description Caffeine consumption during pregnancy has been shown in the scientific literature to be associated with teratogenicity such as low birth weight, fetal malformations, and miscarriage. However, the morphological alterations of the offspring of dams exposed during pregnancy have not been consistently described, and the mechanisms why they occur remain elusive. Thus, we aimed to characterize the offspring malformations induced by moderate and high doses of caffeine during pregnancy. Dams were divided into three groups: control, moderate (0.3 g/L), and high dose (1.0 g/L) of caffeine, which was provided in the drinking water beginning on gestational day 1 and continuing throughout the entire gestation. At moderate doses, only one of the dams had stillborn pups, although no macroscopic malformations were observed. High doses of caffeine induced significantly more malformations (P<0.001) and early death (before P4). The malformations observed were related to fetal development and cardiovascular alterations, namely bruises, macrocephaly with short limbs, abnormal development (or absence) of head structures and limbs, labial malformations, hydrops fetalis, and poor placental formation. We discussed the proposed mechanisms by which caffeine might induce these phenotypes, which may involve down-regulation of adenosine A1 receptors, and increased mothers’ catecholamines. Our findings further confirm the evidence of the teratogenic effects of high doses of caffeine administered during pregnancy. These findings support the recommendation to avoid caffeine exposure during pregnancy. Keywords: Caffeine; offspring; teratogenicity; pregnancy; hydrops; cardiovascular alterations
publishDate 2017
dc.date.none.fl_str_mv 2017-01-17
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
Avaliado por Pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufrgs.br/index.php/hcpa/article/view/68287
url https://seer.ufrgs.br/index.php/hcpa/article/view/68287
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufrgs.br/index.php/hcpa/article/view/68287/pdf
dc.rights.driver.fl_str_mv Copyright (c) 2017 Clinical and Biomedical Research
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Clinical and Biomedical Research
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv HCPA/FAMED/UFRGS
publisher.none.fl_str_mv HCPA/FAMED/UFRGS
dc.source.none.fl_str_mv Clinical & Biomedical Research; Vol. 36 No. 4 (2016)
Clinical and Biomedical Research; v. 36 n. 4 (2016)
2357-9730
reponame:Clinical and Biomedical Research
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Clinical and Biomedical Research
collection Clinical and Biomedical Research
repository.name.fl_str_mv Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)
repository.mail.fl_str_mv ||cbr@hcpa.edu.br
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