Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/218924 |
Resumo: | Campylobacter jejuni is one of the most common causes of foodborne diseases worldwide. There are few reports on Campylobacter strains isolated from Latin-American countries. Here, 140 C. jejuni strains isolated from cloacal and transport boxes swabs, water from chiller tanks, and broiler carcasses of five poultry companies in Southern Brazil were identified using phenotypic and genotypic methods. Polymerase chain reaction (PCR) was used to analyze eight C. jejuni virulence markers: flaA, cadF, and invasion-associated (iam) genes, cdtABC operon (associated with the cytolethal distending toxin), and plasmidial virB11 and wlaN genes were present in 78.5%, 77.8%, 0%, 74.2%, 22.1%, and 10.7% of samples, respectively. There were 25 different virulence profiles: 1 (cdtA, cdtB, cdtC, flaA, and cadF), 2 (cdtA, cdtB, cdtC, flaA, cadF, and virB11), and 3 (cdtA, cdtB, cdtC, flaA, cadF, and wlaN) were the most common (> 60% of strains). We provide insight into factors related to the occurrence of this pathogen and their epidemiology. |
id |
UFRGS-2_0b0d3e905d849ea0ddf864ab2b18cb13 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/218924 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Sierra Arguello, Yuli MelisaPerdoncini, GustavoRodrigues, Laura BeatrizSantos, Luciana Ruschel dosBorges, Karen ApellanisFurian, Thales QuediSalle, Carlos Tadeu PippiMoraes, Hamilton Luiz de SouzaGomes, Marcos José PereiraNascimento, Vladimir Pinheiro do2021-03-16T04:26:39Z20212045-2322http://hdl.handle.net/10183/218924001123311Campylobacter jejuni is one of the most common causes of foodborne diseases worldwide. There are few reports on Campylobacter strains isolated from Latin-American countries. Here, 140 C. jejuni strains isolated from cloacal and transport boxes swabs, water from chiller tanks, and broiler carcasses of five poultry companies in Southern Brazil were identified using phenotypic and genotypic methods. Polymerase chain reaction (PCR) was used to analyze eight C. jejuni virulence markers: flaA, cadF, and invasion-associated (iam) genes, cdtABC operon (associated with the cytolethal distending toxin), and plasmidial virB11 and wlaN genes were present in 78.5%, 77.8%, 0%, 74.2%, 22.1%, and 10.7% of samples, respectively. There were 25 different virulence profiles: 1 (cdtA, cdtB, cdtC, flaA, and cadF), 2 (cdtA, cdtB, cdtC, flaA, cadF, and virB11), and 3 (cdtA, cdtB, cdtC, flaA, cadF, and wlaN) were the most common (> 60% of strains). We provide insight into factors related to the occurrence of this pathogen and their epidemiology.application/pdfengScientific reports. London. Vol. 11 (2021), 4588, 8 p.Campylobacter jejuniFatores de virulênciaFrangos de corteAbatedouroBrasil, Região SulReação em cadeia da polimeraseIdentification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhousesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001123311.pdf.txt001123311.pdf.txtExtracted Texttext/plain47660http://www.lume.ufrgs.br/bitstream/10183/218924/2/001123311.pdf.txt0610ea25b8e702fe7c02cceff25da15bMD52ORIGINAL001123311.pdfTexto completo (inglês)application/pdf1092657http://www.lume.ufrgs.br/bitstream/10183/218924/1/001123311.pdf9599ff0edaa733049f11ac4e739cd7d8MD5110183/2189242021-05-07 05:05:51.741955oai:www.lume.ufrgs.br:10183/218924Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-05-07T08:05:51Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses |
title |
Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses |
spellingShingle |
Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses Sierra Arguello, Yuli Melisa Campylobacter jejuni Fatores de virulência Frangos de corte Abatedouro Brasil, Região Sul Reação em cadeia da polimerase |
title_short |
Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses |
title_full |
Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses |
title_fullStr |
Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses |
title_full_unstemmed |
Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses |
title_sort |
Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses |
author |
Sierra Arguello, Yuli Melisa |
author_facet |
Sierra Arguello, Yuli Melisa Perdoncini, Gustavo Rodrigues, Laura Beatriz Santos, Luciana Ruschel dos Borges, Karen Apellanis Furian, Thales Quedi Salle, Carlos Tadeu Pippi Moraes, Hamilton Luiz de Souza Gomes, Marcos José Pereira Nascimento, Vladimir Pinheiro do |
author_role |
author |
author2 |
Perdoncini, Gustavo Rodrigues, Laura Beatriz Santos, Luciana Ruschel dos Borges, Karen Apellanis Furian, Thales Quedi Salle, Carlos Tadeu Pippi Moraes, Hamilton Luiz de Souza Gomes, Marcos José Pereira Nascimento, Vladimir Pinheiro do |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sierra Arguello, Yuli Melisa Perdoncini, Gustavo Rodrigues, Laura Beatriz Santos, Luciana Ruschel dos Borges, Karen Apellanis Furian, Thales Quedi Salle, Carlos Tadeu Pippi Moraes, Hamilton Luiz de Souza Gomes, Marcos José Pereira Nascimento, Vladimir Pinheiro do |
dc.subject.por.fl_str_mv |
Campylobacter jejuni Fatores de virulência Frangos de corte Abatedouro Brasil, Região Sul Reação em cadeia da polimerase |
topic |
Campylobacter jejuni Fatores de virulência Frangos de corte Abatedouro Brasil, Região Sul Reação em cadeia da polimerase |
description |
Campylobacter jejuni is one of the most common causes of foodborne diseases worldwide. There are few reports on Campylobacter strains isolated from Latin-American countries. Here, 140 C. jejuni strains isolated from cloacal and transport boxes swabs, water from chiller tanks, and broiler carcasses of five poultry companies in Southern Brazil were identified using phenotypic and genotypic methods. Polymerase chain reaction (PCR) was used to analyze eight C. jejuni virulence markers: flaA, cadF, and invasion-associated (iam) genes, cdtABC operon (associated with the cytolethal distending toxin), and plasmidial virB11 and wlaN genes were present in 78.5%, 77.8%, 0%, 74.2%, 22.1%, and 10.7% of samples, respectively. There were 25 different virulence profiles: 1 (cdtA, cdtB, cdtC, flaA, and cadF), 2 (cdtA, cdtB, cdtC, flaA, cadF, and virB11), and 3 (cdtA, cdtB, cdtC, flaA, cadF, and wlaN) were the most common (> 60% of strains). We provide insight into factors related to the occurrence of this pathogen and their epidemiology. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-03-16T04:26:39Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/218924 |
dc.identifier.issn.pt_BR.fl_str_mv |
2045-2322 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001123311 |
identifier_str_mv |
2045-2322 001123311 |
url |
http://hdl.handle.net/10183/218924 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Scientific reports. London. Vol. 11 (2021), 4588, 8 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/218924/2/001123311.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/218924/1/001123311.pdf |
bitstream.checksum.fl_str_mv |
0610ea25b8e702fe7c02cceff25da15b 9599ff0edaa733049f11ac4e739cd7d8 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801225011533447168 |