Reparo de hérnia abdominal com pericárdio bovino associado a células-tronco mesenquimais em ratos Wistar
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/223669 |
Resumo: | Background: Biological membranes demonstrate superiority over synthetic ones for its biocompatibility and strength in the reduction of abdominal hernias. Recents tissue engineering researches add mesenchymal stem cells to biological membranes with the purpose of obtaining additional cellular proliferation and consequent muscle regeneration, using biological membranes as cellular scaffolds. This article aimed to study the infl uence of mesenchymal stem cells in muscle regeneration in abdominal hernias, reduced with biological membranes. Materials, Methods & Results: Adult Wistar rats underwent abdominal hernia-inducing. They were divided into two groups as to the form of treatment for the reduction of hernia: stem cells associated with biological membranes or only biological membranes. After the treatment the macro and microscopic reviews were carried out in days seven, 14 and 60 postoperatively. Preparation of bovine pericardium with glycerin 98% presented effi ciency in decellularization and conservation, maintaining its strength and avoiding bacterial growth. The mesenchymal stem cells obtained from bone marrow of adult Wistar rats, had capacity of proliferation. The majority of the cells was positive for the expression of surface antigens CD44, CD29 and CD99 and was negative for CD 34. In the differentiation trials, the same cells were able to differentiate into adipocytes and osteocytes. With 24 h from co-cultivating adhesion of mesenchymal stem cells in the membranes was observed. There was no foreign body reaction or contamination of surgical wounds and there was intense postoperative neovascularization on seven days. All animals presented omentum adherence, but no adherence to other organs. There was no statistically difference for the different times in macroscopic assessment: deposition of fi brous tissue, implant integration. The same occurred with the microscopic evaluations between the different treatment groups. The groups of immediate and later repair presented different responses to treatment. Discussion: The use of rats as animal model was satisfactory, being suitable for surgical procedures and assessments of the abdominal cavity. The different results obtained between groups of immediate repair and late repair corroborate with the idea that there is difference between induction and repair models in the same surgery or in different surgeries with the time interval between the two, suggesting the need for methodologies that simulate the hernias chronicity. The cells used were classifi ed as mesenchymal stem cells, because it met all the criteria of Mesenchymal and Tissue Stem Cell Committee of the International Society of Celullar Therapy. The membranes conserved with glycerin 98% demonstrated biocompatibility, because there was no rejection or necrosis, infection or exacerbated infl ammation. However the muscle regeneration was not obtained over the membranes - and the methodological difference in other latest experiments about the membranes decellularization and the co-cultivating - can leads to conclusion that the cells attached to membranes were insuffi cient in number to obtain the desired result. These results suggest the need of new research studies or co-cultivating times and decellularization methods of bovine pericardium for association with mesenchymal stem cells. |
id |
UFRGS-2_11f45c0d224144e07187713ad29ffab7 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/223669 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Vidor, Silvana BelliniMarques, Janete Maria VolpatoMoura, Lanucha Fidelis da LuzGomes, CristianoPaz, Ana Helena da RosaGomes, Herbert MartinsMeurer, LuíseBarros, Rodrigo Rodrigues deGuimarães, Karina MaganoCirne Lima, Elizabeth ObinoContesini, Emerson Antônio2021-07-13T04:38:10Z20131678-0345http://hdl.handle.net/10183/223669000917390Background: Biological membranes demonstrate superiority over synthetic ones for its biocompatibility and strength in the reduction of abdominal hernias. Recents tissue engineering researches add mesenchymal stem cells to biological membranes with the purpose of obtaining additional cellular proliferation and consequent muscle regeneration, using biological membranes as cellular scaffolds. This article aimed to study the infl uence of mesenchymal stem cells in muscle regeneration in abdominal hernias, reduced with biological membranes. Materials, Methods & Results: Adult Wistar rats underwent abdominal hernia-inducing. They were divided into two groups as to the form of treatment for the reduction of hernia: stem cells associated with biological membranes or only biological membranes. After the treatment the macro and microscopic reviews were carried out in days seven, 14 and 60 postoperatively. Preparation of bovine pericardium with glycerin 98% presented effi ciency in decellularization and conservation, maintaining its strength and avoiding bacterial growth. The mesenchymal stem cells obtained from bone marrow of adult Wistar rats, had capacity of proliferation. The majority of the cells was positive for the expression of surface antigens CD44, CD29 and CD99 and was negative for CD 34. In the differentiation trials, the same cells were able to differentiate into adipocytes and osteocytes. With 24 h from co-cultivating adhesion of mesenchymal stem cells in the membranes was observed. There was no foreign body reaction or contamination of surgical wounds and there was intense postoperative neovascularization on seven days. All animals presented omentum adherence, but no adherence to other organs. There was no statistically difference for the different times in macroscopic assessment: deposition of fi brous tissue, implant integration. The same occurred with the microscopic evaluations between the different treatment groups. The groups of immediate and later repair presented different responses to treatment. Discussion: The use of rats as animal model was satisfactory, being suitable for surgical procedures and assessments of the abdominal cavity. The different results obtained between groups of immediate repair and late repair corroborate with the idea that there is difference between induction and repair models in the same surgery or in different surgeries with the time interval between the two, suggesting the need for methodologies that simulate the hernias chronicity. The cells used were classifi ed as mesenchymal stem cells, because it met all the criteria of Mesenchymal and Tissue Stem Cell Committee of the International Society of Celullar Therapy. The membranes conserved with glycerin 98% demonstrated biocompatibility, because there was no rejection or necrosis, infection or exacerbated infl ammation. However the muscle regeneration was not obtained over the membranes - and the methodological difference in other latest experiments about the membranes decellularization and the co-cultivating - can leads to conclusion that the cells attached to membranes were insuffi cient in number to obtain the desired result. These results suggest the need of new research studies or co-cultivating times and decellularization methods of bovine pericardium for association with mesenchymal stem cells.application/pdfporActa scientiae veterinariae. Porto Alegre. Vol. 41, artigo 1102 (2013), 10 p.Células-tronco mesenquimaisMembranas (Biologia)BiomateriaisCellular therapyBiological membranesWound healingTissue engineeringMuscleReparo de hérnia abdominal com pericárdio bovino associado a células-tronco mesenquimais em ratos WistarAbdominal hernia repair with bovine pericardium seeded with mesenchymal stem cells in Wistar rats info:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000917390.pdf.txt000917390.pdf.txtExtracted Texttext/plain43536http://www.lume.ufrgs.br/bitstream/10183/223669/2/000917390.pdf.txtbcf02c13f3da6ffb57fd377c9b27a061MD52ORIGINAL000917390.pdfTexto completoapplication/pdf822354http://www.lume.ufrgs.br/bitstream/10183/223669/1/000917390.pdff47fb44b08aebf79d414e34cfc53fb84MD5110183/2236692023-03-03 03:24:24.174729oai:www.lume.ufrgs.br:10183/223669Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-03-03T06:24:24Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Reparo de hérnia abdominal com pericárdio bovino associado a células-tronco mesenquimais em ratos Wistar |
dc.title.alternative.en.fl_str_mv |
Abdominal hernia repair with bovine pericardium seeded with mesenchymal stem cells in Wistar rats |
title |
Reparo de hérnia abdominal com pericárdio bovino associado a células-tronco mesenquimais em ratos Wistar |
spellingShingle |
Reparo de hérnia abdominal com pericárdio bovino associado a células-tronco mesenquimais em ratos Wistar Vidor, Silvana Bellini Células-tronco mesenquimais Membranas (Biologia) Biomateriais Cellular therapy Biological membranes Wound healing Tissue engineering Muscle |
title_short |
Reparo de hérnia abdominal com pericárdio bovino associado a células-tronco mesenquimais em ratos Wistar |
title_full |
Reparo de hérnia abdominal com pericárdio bovino associado a células-tronco mesenquimais em ratos Wistar |
title_fullStr |
Reparo de hérnia abdominal com pericárdio bovino associado a células-tronco mesenquimais em ratos Wistar |
title_full_unstemmed |
Reparo de hérnia abdominal com pericárdio bovino associado a células-tronco mesenquimais em ratos Wistar |
title_sort |
Reparo de hérnia abdominal com pericárdio bovino associado a células-tronco mesenquimais em ratos Wistar |
author |
Vidor, Silvana Bellini |
author_facet |
Vidor, Silvana Bellini Marques, Janete Maria Volpato Moura, Lanucha Fidelis da Luz Gomes, Cristiano Paz, Ana Helena da Rosa Gomes, Herbert Martins Meurer, Luíse Barros, Rodrigo Rodrigues de Guimarães, Karina Magano Cirne Lima, Elizabeth Obino Contesini, Emerson Antônio |
author_role |
author |
author2 |
Marques, Janete Maria Volpato Moura, Lanucha Fidelis da Luz Gomes, Cristiano Paz, Ana Helena da Rosa Gomes, Herbert Martins Meurer, Luíse Barros, Rodrigo Rodrigues de Guimarães, Karina Magano Cirne Lima, Elizabeth Obino Contesini, Emerson Antônio |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vidor, Silvana Bellini Marques, Janete Maria Volpato Moura, Lanucha Fidelis da Luz Gomes, Cristiano Paz, Ana Helena da Rosa Gomes, Herbert Martins Meurer, Luíse Barros, Rodrigo Rodrigues de Guimarães, Karina Magano Cirne Lima, Elizabeth Obino Contesini, Emerson Antônio |
dc.subject.por.fl_str_mv |
Células-tronco mesenquimais Membranas (Biologia) Biomateriais |
topic |
Células-tronco mesenquimais Membranas (Biologia) Biomateriais Cellular therapy Biological membranes Wound healing Tissue engineering Muscle |
dc.subject.eng.fl_str_mv |
Cellular therapy Biological membranes Wound healing Tissue engineering Muscle |
description |
Background: Biological membranes demonstrate superiority over synthetic ones for its biocompatibility and strength in the reduction of abdominal hernias. Recents tissue engineering researches add mesenchymal stem cells to biological membranes with the purpose of obtaining additional cellular proliferation and consequent muscle regeneration, using biological membranes as cellular scaffolds. This article aimed to study the infl uence of mesenchymal stem cells in muscle regeneration in abdominal hernias, reduced with biological membranes. Materials, Methods & Results: Adult Wistar rats underwent abdominal hernia-inducing. They were divided into two groups as to the form of treatment for the reduction of hernia: stem cells associated with biological membranes or only biological membranes. After the treatment the macro and microscopic reviews were carried out in days seven, 14 and 60 postoperatively. Preparation of bovine pericardium with glycerin 98% presented effi ciency in decellularization and conservation, maintaining its strength and avoiding bacterial growth. The mesenchymal stem cells obtained from bone marrow of adult Wistar rats, had capacity of proliferation. The majority of the cells was positive for the expression of surface antigens CD44, CD29 and CD99 and was negative for CD 34. In the differentiation trials, the same cells were able to differentiate into adipocytes and osteocytes. With 24 h from co-cultivating adhesion of mesenchymal stem cells in the membranes was observed. There was no foreign body reaction or contamination of surgical wounds and there was intense postoperative neovascularization on seven days. All animals presented omentum adherence, but no adherence to other organs. There was no statistically difference for the different times in macroscopic assessment: deposition of fi brous tissue, implant integration. The same occurred with the microscopic evaluations between the different treatment groups. The groups of immediate and later repair presented different responses to treatment. Discussion: The use of rats as animal model was satisfactory, being suitable for surgical procedures and assessments of the abdominal cavity. The different results obtained between groups of immediate repair and late repair corroborate with the idea that there is difference between induction and repair models in the same surgery or in different surgeries with the time interval between the two, suggesting the need for methodologies that simulate the hernias chronicity. The cells used were classifi ed as mesenchymal stem cells, because it met all the criteria of Mesenchymal and Tissue Stem Cell Committee of the International Society of Celullar Therapy. The membranes conserved with glycerin 98% demonstrated biocompatibility, because there was no rejection or necrosis, infection or exacerbated infl ammation. However the muscle regeneration was not obtained over the membranes - and the methodological difference in other latest experiments about the membranes decellularization and the co-cultivating - can leads to conclusion that the cells attached to membranes were insuffi cient in number to obtain the desired result. These results suggest the need of new research studies or co-cultivating times and decellularization methods of bovine pericardium for association with mesenchymal stem cells. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2021-07-13T04:38:10Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/223669 |
dc.identifier.issn.pt_BR.fl_str_mv |
1678-0345 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000917390 |
identifier_str_mv |
1678-0345 000917390 |
url |
http://hdl.handle.net/10183/223669 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Acta scientiae veterinariae. Porto Alegre. Vol. 41, artigo 1102 (2013), 10 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/223669/2/000917390.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/223669/1/000917390.pdf |
bitstream.checksum.fl_str_mv |
bcf02c13f3da6ffb57fd377c9b27a061 f47fb44b08aebf79d414e34cfc53fb84 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801225024814710784 |