LPS levels in root canals after the use of ozone gas and high frequency electrical pulses
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/147051 |
Resumo: | The present study aims to verify the effect of ozone gas (OZY® System) and high frequency electric pulse (Endox® System) systems on human root canals previously contaminated with Escherichia coli lipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths standardized to 16 mm. The root canals were prepared up to #60 hand K-files and sterilized using gamma radiation with cobalt 60. The specimens were divided into the following five groups (n = 10) based on the disinfection protocol used: OZY® System, one 120-second-pulse (OZY 1p); OZY® System, four 24-second-pulses (OZY 4p); and Endox® System (ENDOX). Contaminated and non-contaminated canals were exposed only to apyrogenic water and used as positive (C+) and negative (C-) controls, respectively. LPS (O55:B55) was administered in all root canals except those belonging to group C-. After performing disinfection, LPS samples were collected from the canals using apyrogenic paper tips. Limulus Amoebocyte Lysate (LAL) was used to quantify the LPS levels, and the data obtained was analyzed using one-way ANOVA. The disinfection protocols used were unable to reduce the LPS levels significantly (p = 0.019). The use of ozone gas and high frequency electric pulses was not effective in eliminating LPS from the root canals. |
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Melo, Tiago André Fontoura deGründling, Grasiela Sabrina LonghiMontagner, FranciscoScur, AlcioneSteier, LiviuScarparo, Roberta KochenborgerFigueiredo, Jose Antonio Poli deVier-Pelisser, Fabiana Vieira2016-08-11T02:15:28Z20161806-8324http://hdl.handle.net/10183/147051000988579The present study aims to verify the effect of ozone gas (OZY® System) and high frequency electric pulse (Endox® System) systems on human root canals previously contaminated with Escherichia coli lipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths standardized to 16 mm. The root canals were prepared up to #60 hand K-files and sterilized using gamma radiation with cobalt 60. The specimens were divided into the following five groups (n = 10) based on the disinfection protocol used: OZY® System, one 120-second-pulse (OZY 1p); OZY® System, four 24-second-pulses (OZY 4p); and Endox® System (ENDOX). Contaminated and non-contaminated canals were exposed only to apyrogenic water and used as positive (C+) and negative (C-) controls, respectively. LPS (O55:B55) was administered in all root canals except those belonging to group C-. After performing disinfection, LPS samples were collected from the canals using apyrogenic paper tips. Limulus Amoebocyte Lysate (LAL) was used to quantify the LPS levels, and the data obtained was analyzed using one-way ANOVA. The disinfection protocols used were unable to reduce the LPS levels significantly (p = 0.019). The use of ozone gas and high frequency electric pulses was not effective in eliminating LPS from the root canals.application/pdfporBrazilian oral research. São Paulo. Vol. 30, no. 1 (2016), p. e 19, 1-6EndodontiaMicrobiologiaMateriais odontológicosCanais radiculares : TratamentoEndodonticsEndotoxinsMicrobiologyRoot canal therapyLPS levels in root canals after the use of ozone gas and high frequency electrical pulsesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000988579.pdf000988579.pdfTexto completo (inglês)application/pdf405842http://www.lume.ufrgs.br/bitstream/10183/147051/1/000988579.pdf7e0785dc83e9c4ce07a43731f6257856MD51TEXT000988579.pdf.txt000988579.pdf.txtExtracted Texttext/plain24091http://www.lume.ufrgs.br/bitstream/10183/147051/2/000988579.pdf.txt8e051234ad573c55b2f124663b76cb1bMD52THUMBNAIL000988579.pdf.jpg000988579.pdf.jpgGenerated Thumbnailimage/jpeg2052http://www.lume.ufrgs.br/bitstream/10183/147051/3/000988579.pdf.jpgad4e17827bb40b424a8f3074191959c9MD5310183/1470512022-02-22 04:46:03.467053oai:www.lume.ufrgs.br:10183/147051Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-02-22T07:46:03Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
LPS levels in root canals after the use of ozone gas and high frequency electrical pulses |
title |
LPS levels in root canals after the use of ozone gas and high frequency electrical pulses |
spellingShingle |
LPS levels in root canals after the use of ozone gas and high frequency electrical pulses Melo, Tiago André Fontoura de Endodontia Microbiologia Materiais odontológicos Canais radiculares : Tratamento Endodontics Endotoxins Microbiology Root canal therapy |
title_short |
LPS levels in root canals after the use of ozone gas and high frequency electrical pulses |
title_full |
LPS levels in root canals after the use of ozone gas and high frequency electrical pulses |
title_fullStr |
LPS levels in root canals after the use of ozone gas and high frequency electrical pulses |
title_full_unstemmed |
LPS levels in root canals after the use of ozone gas and high frequency electrical pulses |
title_sort |
LPS levels in root canals after the use of ozone gas and high frequency electrical pulses |
author |
Melo, Tiago André Fontoura de |
author_facet |
Melo, Tiago André Fontoura de Gründling, Grasiela Sabrina Longhi Montagner, Francisco Scur, Alcione Steier, Liviu Scarparo, Roberta Kochenborger Figueiredo, Jose Antonio Poli de Vier-Pelisser, Fabiana Vieira |
author_role |
author |
author2 |
Gründling, Grasiela Sabrina Longhi Montagner, Francisco Scur, Alcione Steier, Liviu Scarparo, Roberta Kochenborger Figueiredo, Jose Antonio Poli de Vier-Pelisser, Fabiana Vieira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Melo, Tiago André Fontoura de Gründling, Grasiela Sabrina Longhi Montagner, Francisco Scur, Alcione Steier, Liviu Scarparo, Roberta Kochenborger Figueiredo, Jose Antonio Poli de Vier-Pelisser, Fabiana Vieira |
dc.subject.por.fl_str_mv |
Endodontia Microbiologia Materiais odontológicos Canais radiculares : Tratamento |
topic |
Endodontia Microbiologia Materiais odontológicos Canais radiculares : Tratamento Endodontics Endotoxins Microbiology Root canal therapy |
dc.subject.eng.fl_str_mv |
Endodontics Endotoxins Microbiology Root canal therapy |
description |
The present study aims to verify the effect of ozone gas (OZY® System) and high frequency electric pulse (Endox® System) systems on human root canals previously contaminated with Escherichia coli lipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths standardized to 16 mm. The root canals were prepared up to #60 hand K-files and sterilized using gamma radiation with cobalt 60. The specimens were divided into the following five groups (n = 10) based on the disinfection protocol used: OZY® System, one 120-second-pulse (OZY 1p); OZY® System, four 24-second-pulses (OZY 4p); and Endox® System (ENDOX). Contaminated and non-contaminated canals were exposed only to apyrogenic water and used as positive (C+) and negative (C-) controls, respectively. LPS (O55:B55) was administered in all root canals except those belonging to group C-. After performing disinfection, LPS samples were collected from the canals using apyrogenic paper tips. Limulus Amoebocyte Lysate (LAL) was used to quantify the LPS levels, and the data obtained was analyzed using one-way ANOVA. The disinfection protocols used were unable to reduce the LPS levels significantly (p = 0.019). The use of ozone gas and high frequency electric pulses was not effective in eliminating LPS from the root canals. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-08-11T02:15:28Z |
dc.date.issued.fl_str_mv |
2016 |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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format |
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publishedVersion |
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http://hdl.handle.net/10183/147051 |
dc.identifier.issn.pt_BR.fl_str_mv |
1806-8324 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000988579 |
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http://hdl.handle.net/10183/147051 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian oral research. São Paulo. Vol. 30, no. 1 (2016), p. e 19, 1-6 |
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openAccess |
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