Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Anticarsia gemmatalis (Lepidoptera: Noctuidae)
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/19642 |
Resumo: | The bacterium Bacillus thuringiensis (Bt) is characterized by its ability to produce proteic crystalline inclusions during sporulation. Cry1 protein has insecticidal activity and is highly specific to certain insects and not toxic to unrelated insects, plants or vertebrates. In this work, the patogenicity of twelve Bt isolates was tested against Anticarsia gemmatalis, one of the most important insect pests of soybeans. Spore-crystal complex was applied to the surface of artificial diets and the mortality of A. gemmatalis larvae was assessed seven days after each treatment. When compared to a control Bt isolate known by its high toxicity to A. gemmatalis larvae, four novel Bt isolates exhibited even higher toxic activities against the insect, resulting in more than 90% mortality. PCR was used to amplify DNA fragments related to known cry1 genes. Bt strains with high toxicity produced expected PCR products of around 280 bp, whereas non-toxic or low toxic strains did not produce any PCR product or showed amplified fragments of different sizes. Toxic Bt isolates also exhibited an expected protein profile when total protein extracts were evaluated by SDS-PAGE. |
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Bobrowski, Vera LúciaPasquali, GiancarloBodanese-Zanettini, Maria HelenaFiuza, Lidia Mariana2010-04-16T09:11:02Z20011517-8382http://hdl.handle.net/10183/19642000366659The bacterium Bacillus thuringiensis (Bt) is characterized by its ability to produce proteic crystalline inclusions during sporulation. Cry1 protein has insecticidal activity and is highly specific to certain insects and not toxic to unrelated insects, plants or vertebrates. In this work, the patogenicity of twelve Bt isolates was tested against Anticarsia gemmatalis, one of the most important insect pests of soybeans. Spore-crystal complex was applied to the surface of artificial diets and the mortality of A. gemmatalis larvae was assessed seven days after each treatment. When compared to a control Bt isolate known by its high toxicity to A. gemmatalis larvae, four novel Bt isolates exhibited even higher toxic activities against the insect, resulting in more than 90% mortality. PCR was used to amplify DNA fragments related to known cry1 genes. Bt strains with high toxicity produced expected PCR products of around 280 bp, whereas non-toxic or low toxic strains did not produce any PCR product or showed amplified fragments of different sizes. Toxic Bt isolates also exhibited an expected protein profile when total protein extracts were evaluated by SDS-PAGE.application/pdfengBrazilian journal of microbiology. São Paulo, SP. Vol. 32, n. 2 (abr./jun. 2001), p. 105-109Bacillus thuringiensisAnticarsia gemmatalisGeneSojaInseticidaToxicidadeProteínaBacillus thuringiensisVelvetbean caterpillarAnticarsia gemmatalisCry1 geneDetection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Anticarsia gemmatalis (Lepidoptera: Noctuidae)info:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000366659.pdf000366659.pdfTexto completo (inglês)application/pdf784429http://www.lume.ufrgs.br/bitstream/10183/19642/1/000366659.pdf80c7715c683a0dc14b622255c5d0676fMD51TEXT000366659.pdf.txt000366659.pdf.txtExtracted Texttext/plain25198http://www.lume.ufrgs.br/bitstream/10183/19642/2/000366659.pdf.txte7fd9b178bc1a2ce774a64318e3dc408MD52THUMBNAIL000366659.pdf.jpg000366659.pdf.jpgGenerated Thumbnailimage/jpeg1834http://www.lume.ufrgs.br/bitstream/10183/19642/3/000366659.pdf.jpg5e98b50e8becbe0aa5b87159ff23ff28MD5310183/196422019-03-16 02:31:05.366971oai:www.lume.ufrgs.br:10183/19642Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-03-16T05:31:05Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
title |
Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
spellingShingle |
Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Anticarsia gemmatalis (Lepidoptera: Noctuidae) Bobrowski, Vera Lúcia Bacillus thuringiensis Anticarsia gemmatalis Gene Soja Inseticida Toxicidade Proteína Bacillus thuringiensis Velvetbean caterpillar Anticarsia gemmatalis Cry1 gene |
title_short |
Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
title_full |
Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
title_fullStr |
Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
title_full_unstemmed |
Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
title_sort |
Detection of cry1 genes in Bacillus thuringiensis isolates from South of Brazil and activity against Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
author |
Bobrowski, Vera Lúcia |
author_facet |
Bobrowski, Vera Lúcia Pasquali, Giancarlo Bodanese-Zanettini, Maria Helena Fiuza, Lidia Mariana |
author_role |
author |
author2 |
Pasquali, Giancarlo Bodanese-Zanettini, Maria Helena Fiuza, Lidia Mariana |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Bobrowski, Vera Lúcia Pasquali, Giancarlo Bodanese-Zanettini, Maria Helena Fiuza, Lidia Mariana |
dc.subject.por.fl_str_mv |
Bacillus thuringiensis Anticarsia gemmatalis Gene Soja Inseticida Toxicidade Proteína |
topic |
Bacillus thuringiensis Anticarsia gemmatalis Gene Soja Inseticida Toxicidade Proteína Bacillus thuringiensis Velvetbean caterpillar Anticarsia gemmatalis Cry1 gene |
dc.subject.eng.fl_str_mv |
Bacillus thuringiensis Velvetbean caterpillar Anticarsia gemmatalis Cry1 gene |
description |
The bacterium Bacillus thuringiensis (Bt) is characterized by its ability to produce proteic crystalline inclusions during sporulation. Cry1 protein has insecticidal activity and is highly specific to certain insects and not toxic to unrelated insects, plants or vertebrates. In this work, the patogenicity of twelve Bt isolates was tested against Anticarsia gemmatalis, one of the most important insect pests of soybeans. Spore-crystal complex was applied to the surface of artificial diets and the mortality of A. gemmatalis larvae was assessed seven days after each treatment. When compared to a control Bt isolate known by its high toxicity to A. gemmatalis larvae, four novel Bt isolates exhibited even higher toxic activities against the insect, resulting in more than 90% mortality. PCR was used to amplify DNA fragments related to known cry1 genes. Bt strains with high toxicity produced expected PCR products of around 280 bp, whereas non-toxic or low toxic strains did not produce any PCR product or showed amplified fragments of different sizes. Toxic Bt isolates also exhibited an expected protein profile when total protein extracts were evaluated by SDS-PAGE. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001 |
dc.date.accessioned.fl_str_mv |
2010-04-16T09:11:02Z |
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http://hdl.handle.net/10183/19642 |
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1517-8382 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000366659 |
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http://hdl.handle.net/10183/19642 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of microbiology. São Paulo, SP. Vol. 32, n. 2 (abr./jun. 2001), p. 105-109 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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