Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test

Detalhes bibliográficos
Autor(a) principal: Rozales, Franciéli Pedrotti
Data de Publicação: 2014
Outros Autores: Paris, Fernanda de, Machado, Alice Beatriz Mombach Pinheiro, Costi, Cintia, Barth, Afonso Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/158132
Resumo: Infection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure.
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spelling Rozales, Franciéli PedrottiParis, Fernanda deMachado, Alice Beatriz Mombach PinheiroCosti, CintiaBarth, Afonso Luis2017-05-18T02:40:10Z20142357-9730http://hdl.handle.net/10183/158132000967994Infection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure.application/pdfengClinical and biomedical research. Porto Alegre. Vol. 34, no. 2 (2014), p. 164-168FarmáciaHCVDiagnostic testX-tailAcute diseaseOptimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic testinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000967994.pdf000967994.pdfTexto completo (inglês)application/pdf303103http://www.lume.ufrgs.br/bitstream/10183/158132/1/000967994.pdfe470e0f967ecb1ce019bf787ff3dd714MD51TEXT000967994.pdf.txt000967994.pdf.txtExtracted Texttext/plain15565http://www.lume.ufrgs.br/bitstream/10183/158132/2/000967994.pdf.txt471b51ed977994558f44c3325aa54e78MD52THUMBNAIL000967994.pdf.jpg000967994.pdf.jpgGenerated Thumbnailimage/jpeg1840http://www.lume.ufrgs.br/bitstream/10183/158132/3/000967994.pdf.jpg583fd68a78288b944a2b870292c8b32dMD5310183/1581322018-10-30 08:08:07.038oai:www.lume.ufrgs.br:10183/158132Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-30T11:08:07Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
title Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
spellingShingle Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
Rozales, Franciéli Pedrotti
Farmácia
HCV
Diagnostic test
X-tail
Acute disease
title_short Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
title_full Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
title_fullStr Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
title_full_unstemmed Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
title_sort Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
author Rozales, Franciéli Pedrotti
author_facet Rozales, Franciéli Pedrotti
Paris, Fernanda de
Machado, Alice Beatriz Mombach Pinheiro
Costi, Cintia
Barth, Afonso Luis
author_role author
author2 Paris, Fernanda de
Machado, Alice Beatriz Mombach Pinheiro
Costi, Cintia
Barth, Afonso Luis
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rozales, Franciéli Pedrotti
Paris, Fernanda de
Machado, Alice Beatriz Mombach Pinheiro
Costi, Cintia
Barth, Afonso Luis
dc.subject.por.fl_str_mv Farmácia
topic Farmácia
HCV
Diagnostic test
X-tail
Acute disease
dc.subject.eng.fl_str_mv HCV
Diagnostic test
X-tail
Acute disease
description Infection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2017-05-18T02:40:10Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/158132
dc.identifier.issn.pt_BR.fl_str_mv 2357-9730
dc.identifier.nrb.pt_BR.fl_str_mv 000967994
identifier_str_mv 2357-9730
000967994
url http://hdl.handle.net/10183/158132
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Clinical and biomedical research. Porto Alegre. Vol. 34, no. 2 (2014), p. 164-168
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