Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test
Main Author: | |
---|---|
Publication Date: | 2014 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UFRGS |
Download full: | http://hdl.handle.net/10183/158132 |
Summary: | Infection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure. |
id |
UFRGS-2_1f199abe44337e9104f86f8eb94a2b00 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/158132 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Rozales, Franciéli PedrottiParis, Fernanda deMachado, Alice Beatriz Mombach PinheiroCosti, CintiaBarth, Afonso Luis2017-05-18T02:40:10Z20142357-9730http://hdl.handle.net/10183/158132000967994Infection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure.application/pdfengClinical and biomedical research. Porto Alegre. Vol. 34, no. 2 (2014), p. 164-168FarmáciaHCVDiagnostic testX-tailAcute diseaseOptimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic testinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000967994.pdf000967994.pdfTexto completo (inglês)application/pdf303103http://www.lume.ufrgs.br/bitstream/10183/158132/1/000967994.pdfe470e0f967ecb1ce019bf787ff3dd714MD51TEXT000967994.pdf.txt000967994.pdf.txtExtracted Texttext/plain15565http://www.lume.ufrgs.br/bitstream/10183/158132/2/000967994.pdf.txt471b51ed977994558f44c3325aa54e78MD52THUMBNAIL000967994.pdf.jpg000967994.pdf.jpgGenerated Thumbnailimage/jpeg1840http://www.lume.ufrgs.br/bitstream/10183/158132/3/000967994.pdf.jpg583fd68a78288b944a2b870292c8b32dMD5310183/1581322018-10-30 08:08:07.038oai:www.lume.ufrgs.br:10183/158132Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-30T11:08:07Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test |
title |
Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test |
spellingShingle |
Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test Rozales, Franciéli Pedrotti Farmácia HCV Diagnostic test X-tail Acute disease |
title_short |
Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test |
title_full |
Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test |
title_fullStr |
Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test |
title_full_unstemmed |
Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test |
title_sort |
Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test |
author |
Rozales, Franciéli Pedrotti |
author_facet |
Rozales, Franciéli Pedrotti Paris, Fernanda de Machado, Alice Beatriz Mombach Pinheiro Costi, Cintia Barth, Afonso Luis |
author_role |
author |
author2 |
Paris, Fernanda de Machado, Alice Beatriz Mombach Pinheiro Costi, Cintia Barth, Afonso Luis |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Rozales, Franciéli Pedrotti Paris, Fernanda de Machado, Alice Beatriz Mombach Pinheiro Costi, Cintia Barth, Afonso Luis |
dc.subject.por.fl_str_mv |
Farmácia |
topic |
Farmácia HCV Diagnostic test X-tail Acute disease |
dc.subject.eng.fl_str_mv |
HCV Diagnostic test X-tail Acute disease |
description |
Infection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2017-05-18T02:40:10Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/158132 |
dc.identifier.issn.pt_BR.fl_str_mv |
2357-9730 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000967994 |
identifier_str_mv |
2357-9730 000967994 |
url |
http://hdl.handle.net/10183/158132 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Clinical and biomedical research. Porto Alegre. Vol. 34, no. 2 (2014), p. 164-168 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/158132/1/000967994.pdf http://www.lume.ufrgs.br/bitstream/10183/158132/2/000967994.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/158132/3/000967994.pdf.jpg |
bitstream.checksum.fl_str_mv |
e470e0f967ecb1ce019bf787ff3dd714 471b51ed977994558f44c3325aa54e78 583fd68a78288b944a2b870292c8b32d |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1798487340645089280 |