In vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A)
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/20287 |
Resumo: | This study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion in the glycoprotein E gene (BHV-1.2a gE-). The plaque sizes, penetration and growth kinetics of the Brazilian BHV-1.2a gE- were studied and compared with the parental virus, as well as with a BHV-1.1 gE- recombinant derived from an European BHV-1.1 strain. No statistical differences were observed between the gE- recombinants and the respective parental viruses penetration assays were performed. When single step growth curves were studied, no statistical differences were observed between gE- and parental viruses. However, it was observed that both gE- viruses were excreted from cells in significantly higher titres at 11 hours post infection in comparison with parental viruses. No statistical differences were observed when plaque sizes of parental viruses or gE- viruses we analyzed separately in each cell type. However, both gErecombinants displayed a significantly reduced plaque areas on three different cell cultures, in comparison with parental viruses, indicating that the lack of gE had the same effect on both BHV-1 subtypes, manifested by a restricted cell-to-cell spread in infected cells. |
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Spilki, Fernando RosadoFranco, Ana ClaudiaRijsewijk, Franciscus Antonius MariaWeiblen, RudiFlores, Eduardo FurtadoRoehe, Paulo Michel2010-04-16T09:13:55Z20041517-8382http://hdl.handle.net/10183/20287000575352This study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion in the glycoprotein E gene (BHV-1.2a gE-). The plaque sizes, penetration and growth kinetics of the Brazilian BHV-1.2a gE- were studied and compared with the parental virus, as well as with a BHV-1.1 gE- recombinant derived from an European BHV-1.1 strain. No statistical differences were observed between the gE- recombinants and the respective parental viruses penetration assays were performed. When single step growth curves were studied, no statistical differences were observed between gE- and parental viruses. However, it was observed that both gE- viruses were excreted from cells in significantly higher titres at 11 hours post infection in comparison with parental viruses. No statistical differences were observed when plaque sizes of parental viruses or gE- viruses we analyzed separately in each cell type. However, both gErecombinants displayed a significantly reduced plaque areas on three different cell cultures, in comparison with parental viruses, indicating that the lack of gE had the same effect on both BHV-1 subtypes, manifested by a restricted cell-to-cell spread in infected cells.application/pdfengBrazilian journal of microbiology. São Paulo, SP. Vol. 35, n. 3 (Jul./Set. 2004), p. 264-268Herpesvírus bovinoInfectious bovine rhinotracheitis/infectious pustular vulvovaginitisDifferential vaccineIn vitro growth characteristicsgE- recombinantBHV-1.2aBovine herpesvirusesIn vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A)info:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000575352.pdf000575352.pdfTexto completo (inglês)application/pdf219009http://www.lume.ufrgs.br/bitstream/10183/20287/1/000575352.pdf0268ff8904f67bedfd473d340a03cae5MD51TEXT000575352.pdf.txt000575352.pdf.txtExtracted Texttext/plain20081http://www.lume.ufrgs.br/bitstream/10183/20287/2/000575352.pdf.txtbb0ca72b34e7e46fd33800ac5843a5ddMD52THUMBNAIL000575352.pdf.jpg000575352.pdf.jpgGenerated Thumbnailimage/jpeg1780http://www.lume.ufrgs.br/bitstream/10183/20287/3/000575352.pdf.jpg51ae5a746460ed090e1ea714ec44e80eMD5310183/202872019-01-11 04:09:03.733678oai:www.lume.ufrgs.br:10183/20287Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-01-11T06:09:03Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
In vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A) |
title |
In vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A) |
spellingShingle |
In vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A) Spilki, Fernando Rosado Herpesvírus bovino Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis Differential vaccine In vitro growth characteristics gE- recombinant BHV-1.2a Bovine herpesviruses |
title_short |
In vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A) |
title_full |
In vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A) |
title_fullStr |
In vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A) |
title_full_unstemmed |
In vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A) |
title_sort |
In vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A) |
author |
Spilki, Fernando Rosado |
author_facet |
Spilki, Fernando Rosado Franco, Ana Claudia Rijsewijk, Franciscus Antonius Maria Weiblen, Rudi Flores, Eduardo Furtado Roehe, Paulo Michel |
author_role |
author |
author2 |
Franco, Ana Claudia Rijsewijk, Franciscus Antonius Maria Weiblen, Rudi Flores, Eduardo Furtado Roehe, Paulo Michel |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Spilki, Fernando Rosado Franco, Ana Claudia Rijsewijk, Franciscus Antonius Maria Weiblen, Rudi Flores, Eduardo Furtado Roehe, Paulo Michel |
dc.subject.por.fl_str_mv |
Herpesvírus bovino |
topic |
Herpesvírus bovino Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis Differential vaccine In vitro growth characteristics gE- recombinant BHV-1.2a Bovine herpesviruses |
dc.subject.eng.fl_str_mv |
Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis Differential vaccine In vitro growth characteristics gE- recombinant BHV-1.2a Bovine herpesviruses |
description |
This study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion in the glycoprotein E gene (BHV-1.2a gE-). The plaque sizes, penetration and growth kinetics of the Brazilian BHV-1.2a gE- were studied and compared with the parental virus, as well as with a BHV-1.1 gE- recombinant derived from an European BHV-1.1 strain. No statistical differences were observed between the gE- recombinants and the respective parental viruses penetration assays were performed. When single step growth curves were studied, no statistical differences were observed between gE- and parental viruses. However, it was observed that both gE- viruses were excreted from cells in significantly higher titres at 11 hours post infection in comparison with parental viruses. No statistical differences were observed when plaque sizes of parental viruses or gE- viruses we analyzed separately in each cell type. However, both gErecombinants displayed a significantly reduced plaque areas on three different cell cultures, in comparison with parental viruses, indicating that the lack of gE had the same effect on both BHV-1 subtypes, manifested by a restricted cell-to-cell spread in infected cells. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004 |
dc.date.accessioned.fl_str_mv |
2010-04-16T09:13:55Z |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/20287 |
dc.identifier.issn.pt_BR.fl_str_mv |
1517-8382 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000575352 |
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http://hdl.handle.net/10183/20287 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of microbiology. São Paulo, SP. Vol. 35, n. 3 (Jul./Set. 2004), p. 264-268 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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