Age-dependent availability and functionality of bone marrow stem cells in an experimental model of acute and chronic myocardial infarction
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/189487 |
Resumo: | The aim of this study was to investigate the effect of aging and timing of left ventricular ischemic injury on the availability and functionality of stem cells. We studied young and aged male inbred Lewis rats that were used as donors of bone marrow mononuclear cells (BM-MNCs), divided in four experimental groups: controls, sham operated, 48 h post-myocardial infarction (MI), and 28 days post-MI. In vitro studies included flow cytometry analysis, hematopoietic colony-forming capacity, and invasion assays of migration capacity. BM-MNCs from these groups were transplanted in female rats after MI induction. Late engraftment was evaluated by real-time PCR of the SRY chromosome. Percentage of CD34+/CD45+low cells was similar among different experimental groups in young rats, but was significantly higher in aged animals (p < 0.001), particularly 28 days post-MI. KDR+/CD34+ cells were increased 48 h after MI and decreased 28 days post-MI in young animals, while they were profoundly reduced in the aged group (p < 0.001). Triple staining for CD44+/CD29+/CD71+ cells was similar in different groups of aged rats, but we observed an intense increase 48 h post-MI in young animals. Colony-forming units and cytokine-induced migration were significantly attenuated 28 days after the MI. Late engraftment in infarcted transplanted female hearts was present, but considerably heterogeneous. Finally, recovery of left ventricular systolic function in transplanted female recipients was significantly influenced by donors’ BM-MNCs groups (p < 0.01). We have demonstrated that aging and timing of myocardial injury are factors that may act synergistically in determining stem cell availability and function. Such interaction should be considered when planning new cell therapy strategies for acute and chronic ischemic heart disease in the clinical arena. |
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Lugo, Ana Ilda AyalaTavares, Angela Maria VicentePaz, Ana Helena da RosaAlegretti, Ana PaulaMiquelito, Ludmila do ValeBock, HugoGiugliani, RobertoClausell, Nadine OliveiraCirne Lima, Elizabeth ObinoRohde, Luis Eduardo Paim2019-03-21T02:29:30Z20110963-6897http://hdl.handle.net/10183/189487000784457The aim of this study was to investigate the effect of aging and timing of left ventricular ischemic injury on the availability and functionality of stem cells. We studied young and aged male inbred Lewis rats that were used as donors of bone marrow mononuclear cells (BM-MNCs), divided in four experimental groups: controls, sham operated, 48 h post-myocardial infarction (MI), and 28 days post-MI. In vitro studies included flow cytometry analysis, hematopoietic colony-forming capacity, and invasion assays of migration capacity. BM-MNCs from these groups were transplanted in female rats after MI induction. Late engraftment was evaluated by real-time PCR of the SRY chromosome. Percentage of CD34+/CD45+low cells was similar among different experimental groups in young rats, but was significantly higher in aged animals (p < 0.001), particularly 28 days post-MI. KDR+/CD34+ cells were increased 48 h after MI and decreased 28 days post-MI in young animals, while they were profoundly reduced in the aged group (p < 0.001). Triple staining for CD44+/CD29+/CD71+ cells was similar in different groups of aged rats, but we observed an intense increase 48 h post-MI in young animals. Colony-forming units and cytokine-induced migration were significantly attenuated 28 days after the MI. Late engraftment in infarcted transplanted female hearts was present, but considerably heterogeneous. Finally, recovery of left ventricular systolic function in transplanted female recipients was significantly influenced by donors’ BM-MNCs groups (p < 0.01). We have demonstrated that aging and timing of myocardial injury are factors that may act synergistically in determining stem cell availability and function. Such interaction should be considered when planning new cell therapy strategies for acute and chronic ischemic heart disease in the clinical arena.application/pdfengCell transplantation. Elmsford. Vol. 20, no. 3 (2011), p. 407-419.Terapia tecidualInfarto do miocárdioEnvelhecimentoModelos animais de doençasCell therapyAgingMyocardial infarction (MI)Age-dependent availability and functionality of bone marrow stem cells in an experimental model of acute and chronic myocardial infarctionEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000784457.pdf.txt000784457.pdf.txtExtracted Texttext/plain49861http://www.lume.ufrgs.br/bitstream/10183/189487/2/000784457.pdf.txt2e83cdb47e2d050ada969f11f32f7579MD52ORIGINAL000784457.pdfTexto completo (Inglês)application/pdf857882http://www.lume.ufrgs.br/bitstream/10183/189487/1/000784457.pdffb19d0917fce9f825a671a847a58a8f1MD5110183/1894872019-03-28 04:08:55.827615oai:www.lume.ufrgs.br:10183/189487Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-03-28T07:08:55Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Age-dependent availability and functionality of bone marrow stem cells in an experimental model of acute and chronic myocardial infarction |
title |
Age-dependent availability and functionality of bone marrow stem cells in an experimental model of acute and chronic myocardial infarction |
spellingShingle |
Age-dependent availability and functionality of bone marrow stem cells in an experimental model of acute and chronic myocardial infarction Lugo, Ana Ilda Ayala Terapia tecidual Infarto do miocárdio Envelhecimento Modelos animais de doenças Cell therapy Aging Myocardial infarction (MI) |
title_short |
Age-dependent availability and functionality of bone marrow stem cells in an experimental model of acute and chronic myocardial infarction |
title_full |
Age-dependent availability and functionality of bone marrow stem cells in an experimental model of acute and chronic myocardial infarction |
title_fullStr |
Age-dependent availability and functionality of bone marrow stem cells in an experimental model of acute and chronic myocardial infarction |
title_full_unstemmed |
Age-dependent availability and functionality of bone marrow stem cells in an experimental model of acute and chronic myocardial infarction |
title_sort |
Age-dependent availability and functionality of bone marrow stem cells in an experimental model of acute and chronic myocardial infarction |
author |
Lugo, Ana Ilda Ayala |
author_facet |
Lugo, Ana Ilda Ayala Tavares, Angela Maria Vicente Paz, Ana Helena da Rosa Alegretti, Ana Paula Miquelito, Ludmila do Vale Bock, Hugo Giugliani, Roberto Clausell, Nadine Oliveira Cirne Lima, Elizabeth Obino Rohde, Luis Eduardo Paim |
author_role |
author |
author2 |
Tavares, Angela Maria Vicente Paz, Ana Helena da Rosa Alegretti, Ana Paula Miquelito, Ludmila do Vale Bock, Hugo Giugliani, Roberto Clausell, Nadine Oliveira Cirne Lima, Elizabeth Obino Rohde, Luis Eduardo Paim |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lugo, Ana Ilda Ayala Tavares, Angela Maria Vicente Paz, Ana Helena da Rosa Alegretti, Ana Paula Miquelito, Ludmila do Vale Bock, Hugo Giugliani, Roberto Clausell, Nadine Oliveira Cirne Lima, Elizabeth Obino Rohde, Luis Eduardo Paim |
dc.subject.por.fl_str_mv |
Terapia tecidual Infarto do miocárdio Envelhecimento Modelos animais de doenças |
topic |
Terapia tecidual Infarto do miocárdio Envelhecimento Modelos animais de doenças Cell therapy Aging Myocardial infarction (MI) |
dc.subject.eng.fl_str_mv |
Cell therapy Aging Myocardial infarction (MI) |
description |
The aim of this study was to investigate the effect of aging and timing of left ventricular ischemic injury on the availability and functionality of stem cells. We studied young and aged male inbred Lewis rats that were used as donors of bone marrow mononuclear cells (BM-MNCs), divided in four experimental groups: controls, sham operated, 48 h post-myocardial infarction (MI), and 28 days post-MI. In vitro studies included flow cytometry analysis, hematopoietic colony-forming capacity, and invasion assays of migration capacity. BM-MNCs from these groups were transplanted in female rats after MI induction. Late engraftment was evaluated by real-time PCR of the SRY chromosome. Percentage of CD34+/CD45+low cells was similar among different experimental groups in young rats, but was significantly higher in aged animals (p < 0.001), particularly 28 days post-MI. KDR+/CD34+ cells were increased 48 h after MI and decreased 28 days post-MI in young animals, while they were profoundly reduced in the aged group (p < 0.001). Triple staining for CD44+/CD29+/CD71+ cells was similar in different groups of aged rats, but we observed an intense increase 48 h post-MI in young animals. Colony-forming units and cytokine-induced migration were significantly attenuated 28 days after the MI. Late engraftment in infarcted transplanted female hearts was present, but considerably heterogeneous. Finally, recovery of left ventricular systolic function in transplanted female recipients was significantly influenced by donors’ BM-MNCs groups (p < 0.01). We have demonstrated that aging and timing of myocardial injury are factors that may act synergistically in determining stem cell availability and function. Such interaction should be considered when planning new cell therapy strategies for acute and chronic ischemic heart disease in the clinical arena. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2019-03-21T02:29:30Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/189487 |
dc.identifier.issn.pt_BR.fl_str_mv |
0963-6897 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000784457 |
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0963-6897 000784457 |
url |
http://hdl.handle.net/10183/189487 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Cell transplantation. Elmsford. Vol. 20, no. 3 (2011), p. 407-419. |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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