Identification, antimicrobial resistance and genotypic characterization of enterococcus spp. isolated in Porto Alegre, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/23958 |
Resumo: | In the past two decades the members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. In the present study, we evaluated the antimicrobial resistance and genotypic characteristics of 203 Enterococcus spp. recovered from different clinical sources from two hospitals in Porto Alegre, Rio Grande do Sul, Brazil. The species were identified by conventional biochemical tests and by an automated system. The genetic diversity of E. faecalis presenting high-level aminoglycoside resistance (HLAR) was assessed by pulsed-field gel electrophoresis of chromosomal DNA after SmaI digestion. The E. faecalis was the most frequent specie (93.6%), followed by E. faecium (4.4%). The antimicrobial resistance profile was: 2.5% to ampicillin, 0.5% to vancomycin, 0.5% teicoplanin, 33% to chloramphenicol, 2% to nitrofurantoin, 66.1% to erythromycin, 66.5% to tetracycline, 24.6% to rifampicin, 30% to ciprofloxacin and 87.2% to quinupristin-dalfopristin. A total of 10.3% of the isolates proved to be HLAR to both gentamicin and streptomycin (HLR-ST/GE), with 23.6% resistant only to gentamicin (HLR-GE) and 37.4% only to streptomycin (HLR-ST). One predominant clonal group was found among E. faecalis HLR-GE/ST. The prevalence of resistance among beta-lactam antibiotics and glycopeptides was very low. However, in this study there was an increased number of HLR Enterococcus which may be spreading intra and inter-hospital. |
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Bender, Eduardo AndréFreitas, Ana Lucia Peixoto deReiter, Keli CristineLutz, LarissaBarth, Afonso Luis2010-06-19T04:17:48Z20091517-8382http://hdl.handle.net/10183/23958000710305In the past two decades the members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. In the present study, we evaluated the antimicrobial resistance and genotypic characteristics of 203 Enterococcus spp. recovered from different clinical sources from two hospitals in Porto Alegre, Rio Grande do Sul, Brazil. The species were identified by conventional biochemical tests and by an automated system. The genetic diversity of E. faecalis presenting high-level aminoglycoside resistance (HLAR) was assessed by pulsed-field gel electrophoresis of chromosomal DNA after SmaI digestion. The E. faecalis was the most frequent specie (93.6%), followed by E. faecium (4.4%). The antimicrobial resistance profile was: 2.5% to ampicillin, 0.5% to vancomycin, 0.5% teicoplanin, 33% to chloramphenicol, 2% to nitrofurantoin, 66.1% to erythromycin, 66.5% to tetracycline, 24.6% to rifampicin, 30% to ciprofloxacin and 87.2% to quinupristin-dalfopristin. A total of 10.3% of the isolates proved to be HLAR to both gentamicin and streptomycin (HLR-ST/GE), with 23.6% resistant only to gentamicin (HLR-GE) and 37.4% only to streptomycin (HLR-ST). One predominant clonal group was found among E. faecalis HLR-GE/ST. The prevalence of resistance among beta-lactam antibiotics and glycopeptides was very low. However, in this study there was an increased number of HLR Enterococcus which may be spreading intra and inter-hospital.application/pdfengBrazilian journal of microbiology. São Paulo, SP. Vol. 40, n. 3 (set. 2009), p. 693-700EnterococcusResistência bacterianaEnterococcusResistanceHLARPFGEIdentification, antimicrobial resistance and genotypic characterization of enterococcus spp. isolated in Porto Alegre, Brazilinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000710305.pdf.txt000710305.pdf.txtExtracted Texttext/plain25325http://www.lume.ufrgs.br/bitstream/10183/23958/2/000710305.pdf.txt08af80154be35b9c2f95a2d7f21b8febMD52ORIGINAL000710305.pdf000710305.pdfTexto completo (inglês)application/pdf381786http://www.lume.ufrgs.br/bitstream/10183/23958/1/000710305.pdf6cd02af37e06b20c4eddd5758e1596f3MD51THUMBNAIL000710305.pdf.jpg000710305.pdf.jpgGenerated Thumbnailimage/jpeg1901http://www.lume.ufrgs.br/bitstream/10183/23958/3/000710305.pdf.jpgd8926c4dd890edfad11be0b902ff0525MD5310183/239582018-10-09 08:10:53.112oai:www.lume.ufrgs.br:10183/23958Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-09T11:10:53Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Identification, antimicrobial resistance and genotypic characterization of enterococcus spp. isolated in Porto Alegre, Brazil |
title |
Identification, antimicrobial resistance and genotypic characterization of enterococcus spp. isolated in Porto Alegre, Brazil |
spellingShingle |
Identification, antimicrobial resistance and genotypic characterization of enterococcus spp. isolated in Porto Alegre, Brazil Bender, Eduardo André Enterococcus Resistência bacteriana Enterococcus Resistance HLAR PFGE |
title_short |
Identification, antimicrobial resistance and genotypic characterization of enterococcus spp. isolated in Porto Alegre, Brazil |
title_full |
Identification, antimicrobial resistance and genotypic characterization of enterococcus spp. isolated in Porto Alegre, Brazil |
title_fullStr |
Identification, antimicrobial resistance and genotypic characterization of enterococcus spp. isolated in Porto Alegre, Brazil |
title_full_unstemmed |
Identification, antimicrobial resistance and genotypic characterization of enterococcus spp. isolated in Porto Alegre, Brazil |
title_sort |
Identification, antimicrobial resistance and genotypic characterization of enterococcus spp. isolated in Porto Alegre, Brazil |
author |
Bender, Eduardo André |
author_facet |
Bender, Eduardo André Freitas, Ana Lucia Peixoto de Reiter, Keli Cristine Lutz, Larissa Barth, Afonso Luis |
author_role |
author |
author2 |
Freitas, Ana Lucia Peixoto de Reiter, Keli Cristine Lutz, Larissa Barth, Afonso Luis |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Bender, Eduardo André Freitas, Ana Lucia Peixoto de Reiter, Keli Cristine Lutz, Larissa Barth, Afonso Luis |
dc.subject.por.fl_str_mv |
Enterococcus Resistência bacteriana |
topic |
Enterococcus Resistência bacteriana Enterococcus Resistance HLAR PFGE |
dc.subject.eng.fl_str_mv |
Enterococcus Resistance HLAR PFGE |
description |
In the past two decades the members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. In the present study, we evaluated the antimicrobial resistance and genotypic characteristics of 203 Enterococcus spp. recovered from different clinical sources from two hospitals in Porto Alegre, Rio Grande do Sul, Brazil. The species were identified by conventional biochemical tests and by an automated system. The genetic diversity of E. faecalis presenting high-level aminoglycoside resistance (HLAR) was assessed by pulsed-field gel electrophoresis of chromosomal DNA after SmaI digestion. The E. faecalis was the most frequent specie (93.6%), followed by E. faecium (4.4%). The antimicrobial resistance profile was: 2.5% to ampicillin, 0.5% to vancomycin, 0.5% teicoplanin, 33% to chloramphenicol, 2% to nitrofurantoin, 66.1% to erythromycin, 66.5% to tetracycline, 24.6% to rifampicin, 30% to ciprofloxacin and 87.2% to quinupristin-dalfopristin. A total of 10.3% of the isolates proved to be HLAR to both gentamicin and streptomycin (HLR-ST/GE), with 23.6% resistant only to gentamicin (HLR-GE) and 37.4% only to streptomycin (HLR-ST). One predominant clonal group was found among E. faecalis HLR-GE/ST. The prevalence of resistance among beta-lactam antibiotics and glycopeptides was very low. However, in this study there was an increased number of HLR Enterococcus which may be spreading intra and inter-hospital. |
publishDate |
2009 |
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2009 |
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2010-06-19T04:17:48Z |
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dc.language.iso.fl_str_mv |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of microbiology. São Paulo, SP. Vol. 40, n. 3 (set. 2009), p. 693-700 |
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