In vitro and in vivo embryo production efficiency in Flemish and Holstein donor females
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/270915 |
Resumo: | The aim of this study was to compare embryo production efficiency in Flemish and Holstein donor females using ovum pick-up and in vitro fertilization (OPU-IVF) or in vivo production (superovulation; SOV) procedures. The study was conducted using a split-plot design, with eight Flemish and eight Holstein non-lactating cycling females. Females were subjected to ten weekly OPU/IVF sessions and/or two SOV/embryo collections sessions at a 63-day interval, for a total of 160 OPU-IVF and 32 SOV sessions. Mean numbers of follicles and corpora lutea, and cumulus-oocyte complex (COC) recovery rates were similar between breeds after the OPU and SOV sessions. However, Flemish donors yielded better quality grade II COCs (301, 41.9%) than Holstein females (609, and 202, 33.1%). Also, cleavage and blastocyst rates, and the total number and the mean number of viable embryos obtained after OPU-IVF were higher in Flemish (49.6% and 11.8%, and 63 and 11.8 per donor, respectively) than in Holstein (32.8% and 7.2%, and 34 and 7.2 per donor, respectively) females. Flemish females were also more efficient in yielding viable embryos after SOV (111, 7.3 per donor) than Holstein (48, 3.3 per donor) females. Overall, Flemish donor females had better responses to OPU-IVF or SOV procedures than Holstein counterparts. Irrespective of the breeds, SOV procedures were more efficient than OPU-IVF in yielding more viable embryos, under the conditions of this study. Both reproductive procedures were useful tools for the genetic conservation of the Flemish cattle breed in Southern Brazil. |
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Zago, Fabiano CarminattiSchutz, Luis FernandoGerger, Renato Pereira da CostaAguiar, Luis Henrique dePinzón Osorio, César AugustoMezzalira, AlceuRodrigues, José Luiz RigoForell, FabianaBertolini, Marcelo2024-01-12T03:29:25Z20231806-9614http://hdl.handle.net/10183/270915001193295The aim of this study was to compare embryo production efficiency in Flemish and Holstein donor females using ovum pick-up and in vitro fertilization (OPU-IVF) or in vivo production (superovulation; SOV) procedures. The study was conducted using a split-plot design, with eight Flemish and eight Holstein non-lactating cycling females. Females were subjected to ten weekly OPU/IVF sessions and/or two SOV/embryo collections sessions at a 63-day interval, for a total of 160 OPU-IVF and 32 SOV sessions. Mean numbers of follicles and corpora lutea, and cumulus-oocyte complex (COC) recovery rates were similar between breeds after the OPU and SOV sessions. However, Flemish donors yielded better quality grade II COCs (301, 41.9%) than Holstein females (609, and 202, 33.1%). Also, cleavage and blastocyst rates, and the total number and the mean number of viable embryos obtained after OPU-IVF were higher in Flemish (49.6% and 11.8%, and 63 and 11.8 per donor, respectively) than in Holstein (32.8% and 7.2%, and 34 and 7.2 per donor, respectively) females. Flemish females were also more efficient in yielding viable embryos after SOV (111, 7.3 per donor) than Holstein (48, 3.3 per donor) females. Overall, Flemish donor females had better responses to OPU-IVF or SOV procedures than Holstein counterparts. Irrespective of the breeds, SOV procedures were more efficient than OPU-IVF in yielding more viable embryos, under the conditions of this study. Both reproductive procedures were useful tools for the genetic conservation of the Flemish cattle breed in Southern Brazil.application/pdfengAnimal reproduction. Belo Horizonte. Vol. 20, no. 3 (2023), e20230080, 15 p.Desempenho reprodutivoFertilização in vitroSuperovulaçãoEmbrioesRaças de bovinosIn vitro fertilizationOvum pick-upMultiple ovulationBreed conservationCattleIn vitro and in vivo embryo production efficiency in Flemish and Holstein donor femalesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001193295.pdf.txt001193295.pdf.txtExtracted Texttext/plain62765http://www.lume.ufrgs.br/bitstream/10183/270915/2/001193295.pdf.txt7bb10572cb1bd59f5e0517bc2d62f2eeMD52ORIGINAL001193295.pdfTexto completo (inglês)application/pdf578469http://www.lume.ufrgs.br/bitstream/10183/270915/1/001193295.pdfd48b4a9134cdf9f0ed1835cd502b32b7MD5110183/2709152024-01-13 04:42:14.425156oai:www.lume.ufrgs.br:10183/270915Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-01-13T06:42:14Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
In vitro and in vivo embryo production efficiency in Flemish and Holstein donor females |
title |
In vitro and in vivo embryo production efficiency in Flemish and Holstein donor females |
spellingShingle |
In vitro and in vivo embryo production efficiency in Flemish and Holstein donor females Zago, Fabiano Carminatti Desempenho reprodutivo Fertilização in vitro Superovulação Embrioes Raças de bovinos In vitro fertilization Ovum pick-up Multiple ovulation Breed conservation Cattle |
title_short |
In vitro and in vivo embryo production efficiency in Flemish and Holstein donor females |
title_full |
In vitro and in vivo embryo production efficiency in Flemish and Holstein donor females |
title_fullStr |
In vitro and in vivo embryo production efficiency in Flemish and Holstein donor females |
title_full_unstemmed |
In vitro and in vivo embryo production efficiency in Flemish and Holstein donor females |
title_sort |
In vitro and in vivo embryo production efficiency in Flemish and Holstein donor females |
author |
Zago, Fabiano Carminatti |
author_facet |
Zago, Fabiano Carminatti Schutz, Luis Fernando Gerger, Renato Pereira da Costa Aguiar, Luis Henrique de Pinzón Osorio, César Augusto Mezzalira, Alceu Rodrigues, José Luiz Rigo Forell, Fabiana Bertolini, Marcelo |
author_role |
author |
author2 |
Schutz, Luis Fernando Gerger, Renato Pereira da Costa Aguiar, Luis Henrique de Pinzón Osorio, César Augusto Mezzalira, Alceu Rodrigues, José Luiz Rigo Forell, Fabiana Bertolini, Marcelo |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Zago, Fabiano Carminatti Schutz, Luis Fernando Gerger, Renato Pereira da Costa Aguiar, Luis Henrique de Pinzón Osorio, César Augusto Mezzalira, Alceu Rodrigues, José Luiz Rigo Forell, Fabiana Bertolini, Marcelo |
dc.subject.por.fl_str_mv |
Desempenho reprodutivo Fertilização in vitro Superovulação Embrioes Raças de bovinos |
topic |
Desempenho reprodutivo Fertilização in vitro Superovulação Embrioes Raças de bovinos In vitro fertilization Ovum pick-up Multiple ovulation Breed conservation Cattle |
dc.subject.eng.fl_str_mv |
In vitro fertilization Ovum pick-up Multiple ovulation Breed conservation Cattle |
description |
The aim of this study was to compare embryo production efficiency in Flemish and Holstein donor females using ovum pick-up and in vitro fertilization (OPU-IVF) or in vivo production (superovulation; SOV) procedures. The study was conducted using a split-plot design, with eight Flemish and eight Holstein non-lactating cycling females. Females were subjected to ten weekly OPU/IVF sessions and/or two SOV/embryo collections sessions at a 63-day interval, for a total of 160 OPU-IVF and 32 SOV sessions. Mean numbers of follicles and corpora lutea, and cumulus-oocyte complex (COC) recovery rates were similar between breeds after the OPU and SOV sessions. However, Flemish donors yielded better quality grade II COCs (301, 41.9%) than Holstein females (609, and 202, 33.1%). Also, cleavage and blastocyst rates, and the total number and the mean number of viable embryos obtained after OPU-IVF were higher in Flemish (49.6% and 11.8%, and 63 and 11.8 per donor, respectively) than in Holstein (32.8% and 7.2%, and 34 and 7.2 per donor, respectively) females. Flemish females were also more efficient in yielding viable embryos after SOV (111, 7.3 per donor) than Holstein (48, 3.3 per donor) females. Overall, Flemish donor females had better responses to OPU-IVF or SOV procedures than Holstein counterparts. Irrespective of the breeds, SOV procedures were more efficient than OPU-IVF in yielding more viable embryos, under the conditions of this study. Both reproductive procedures were useful tools for the genetic conservation of the Flemish cattle breed in Southern Brazil. |
publishDate |
2023 |
dc.date.issued.fl_str_mv |
2023 |
dc.date.accessioned.fl_str_mv |
2024-01-12T03:29:25Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/270915 |
dc.identifier.issn.pt_BR.fl_str_mv |
1806-9614 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001193295 |
identifier_str_mv |
1806-9614 001193295 |
url |
http://hdl.handle.net/10183/270915 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Animal reproduction. Belo Horizonte. Vol. 20, no. 3 (2023), e20230080, 15 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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