Antibiotic resistance and molecular typing of Pseudomonas aeruginosa : focus on imipenem
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/37735 |
Resumo: | Susceptibility tests by disk diffusion and by E-test and molecular typing by macrorestriction analysis were performed to determine the relatedness of Pseudomonas aeruginosa isolates from three distinct hospitals. The resistance profile of 124 isolates to 8 antimicrobial agents was determined in three different hospitals, in Porto Alegre, Brazil. Frequencies of susceptibility ranged from 43.9% for carbenicillin to 87.7% for ceftazidime. Cross-resistance data of imipenem-resistant isolates indicated that most (70%) were also resistant to carbenicillin, although 30% remained susceptible to ceftazidime and cefepime. In general, susceptibility profiles were not able to determine relatedness among isolates of P. aeruginosa. On the other hand, molecular typing by macrorestriction analysis demonstrated high discriminatory power and identified 66 strains among 72 isolates of P. aeruginosa. Imipenem-susceptible isolates were all different. However, identical clones of imipenem-resistant isolates were found in two of the hospitals, despite variable response to other antibiotics. No clustering of infection among the different medical centers was observed. In conclusion, clones of P. aeruginosa did not spread among the different hospitals in our city even though related isolates of imipenem- resistant P. aeruginosa were found. |
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Freitas, Ana Lucia Peixoto deBarth, Afonso Luis2012-03-23T01:20:00Z20021413-8670http://hdl.handle.net/10183/37735000318529Susceptibility tests by disk diffusion and by E-test and molecular typing by macrorestriction analysis were performed to determine the relatedness of Pseudomonas aeruginosa isolates from three distinct hospitals. The resistance profile of 124 isolates to 8 antimicrobial agents was determined in three different hospitals, in Porto Alegre, Brazil. Frequencies of susceptibility ranged from 43.9% for carbenicillin to 87.7% for ceftazidime. Cross-resistance data of imipenem-resistant isolates indicated that most (70%) were also resistant to carbenicillin, although 30% remained susceptible to ceftazidime and cefepime. In general, susceptibility profiles were not able to determine relatedness among isolates of P. aeruginosa. On the other hand, molecular typing by macrorestriction analysis demonstrated high discriminatory power and identified 66 strains among 72 isolates of P. aeruginosa. Imipenem-susceptible isolates were all different. However, identical clones of imipenem-resistant isolates were found in two of the hospitals, despite variable response to other antibiotics. No clustering of infection among the different medical centers was observed. In conclusion, clones of P. aeruginosa did not spread among the different hospitals in our city even though related isolates of imipenem- resistant P. aeruginosa were found.application/pdfengThe Brazilian journal of infectious diseases. Vol. 6, n. 1 (fev. 2002), p. 1-7Pseudomonas aeruginosaImipenemResistência bacterianaPseudomonas aeruginosaAntibiotic resistanceImipenemAntibiotic resistance and molecular typing of Pseudomonas aeruginosa : focus on imipeneminfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000318529.pdf000318529.pdfTexto completo (inglês)application/pdf1271472http://www.lume.ufrgs.br/bitstream/10183/37735/1/000318529.pdf1af84a2498408773915976fc3f2882e9MD51TEXT000318529.pdf.txt000318529.pdf.txtExtracted Texttext/plain21124http://www.lume.ufrgs.br/bitstream/10183/37735/2/000318529.pdf.txt5ad39daa46c70ce1f8e1d6ad3262d25eMD52THUMBNAIL000318529.pdf.jpg000318529.pdf.jpgGenerated Thumbnailimage/jpeg1908http://www.lume.ufrgs.br/bitstream/10183/37735/3/000318529.pdf.jpgef4214d46d2d4ded3f30b289bfab04e5MD5310183/377352018-11-01 02:49:50.0827oai:www.lume.ufrgs.br:10183/37735Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-11-01T05:49:50Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa : focus on imipenem |
title |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa : focus on imipenem |
spellingShingle |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa : focus on imipenem Freitas, Ana Lucia Peixoto de Pseudomonas aeruginosa Imipenem Resistência bacteriana Pseudomonas aeruginosa Antibiotic resistance Imipenem |
title_short |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa : focus on imipenem |
title_full |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa : focus on imipenem |
title_fullStr |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa : focus on imipenem |
title_full_unstemmed |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa : focus on imipenem |
title_sort |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa : focus on imipenem |
author |
Freitas, Ana Lucia Peixoto de |
author_facet |
Freitas, Ana Lucia Peixoto de Barth, Afonso Luis |
author_role |
author |
author2 |
Barth, Afonso Luis |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Freitas, Ana Lucia Peixoto de Barth, Afonso Luis |
dc.subject.por.fl_str_mv |
Pseudomonas aeruginosa Imipenem Resistência bacteriana |
topic |
Pseudomonas aeruginosa Imipenem Resistência bacteriana Pseudomonas aeruginosa Antibiotic resistance Imipenem |
dc.subject.eng.fl_str_mv |
Pseudomonas aeruginosa Antibiotic resistance Imipenem |
description |
Susceptibility tests by disk diffusion and by E-test and molecular typing by macrorestriction analysis were performed to determine the relatedness of Pseudomonas aeruginosa isolates from three distinct hospitals. The resistance profile of 124 isolates to 8 antimicrobial agents was determined in three different hospitals, in Porto Alegre, Brazil. Frequencies of susceptibility ranged from 43.9% for carbenicillin to 87.7% for ceftazidime. Cross-resistance data of imipenem-resistant isolates indicated that most (70%) were also resistant to carbenicillin, although 30% remained susceptible to ceftazidime and cefepime. In general, susceptibility profiles were not able to determine relatedness among isolates of P. aeruginosa. On the other hand, molecular typing by macrorestriction analysis demonstrated high discriminatory power and identified 66 strains among 72 isolates of P. aeruginosa. Imipenem-susceptible isolates were all different. However, identical clones of imipenem-resistant isolates were found in two of the hospitals, despite variable response to other antibiotics. No clustering of infection among the different medical centers was observed. In conclusion, clones of P. aeruginosa did not spread among the different hospitals in our city even though related isolates of imipenem- resistant P. aeruginosa were found. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002 |
dc.date.accessioned.fl_str_mv |
2012-03-23T01:20:00Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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info:eu-repo/semantics/publishedVersion |
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article |
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http://hdl.handle.net/10183/37735 |
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1413-8670 |
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000318529 |
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http://hdl.handle.net/10183/37735 |
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eng |
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dc.relation.ispartof.pt_BR.fl_str_mv |
The Brazilian journal of infectious diseases. Vol. 6, n. 1 (fev. 2002), p. 1-7 |
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openAccess |
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