Impact of vitamin D receptor and binding protein gene polymorphisms in clinical and laboratory data of HCV patients : cross sectional study
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/174388 |
Resumo: | Potential relationship of vitamin D, vitamin D receptor (VDR), and vitamin D binding protein (DBP) have been suggested in the pathophysiology of hepatitis C virus (HCV) infection. The aim of this observational study is to determine vitamin D levels, and VDR and DBP genetic polymorphism according demographic and laboratory data in chronic HCV patients (CHC). A total of 148 CHC patients gave serum samples for testing 25-hydroxyvitamin D (25 (OH)D) level by immunochemiluminometric assay (<20ng/mL defined as deficient) and donated blood samples to allelic discrimination analysis using TaqMan assays. Analyzed single nucleotide polymorphisms (SNPs) were: VDR-rs7975232 (ApaI) C>A, rs731236 A>G (TaqI), rs1544410 C>T (BsmI), rs10735810 T>C (FokI) and carrier globulin/binding protein (GC)-rs4588 and rs7041 and the haplotype bAt [CCA]. Hepatic fibrosis was assessed using Fib-4 and Forns index. Eighty-two (54.40%) patients demonstrated deficiency of vitamin D and this was associated to AST (P=.019 [CI: 1.003–1.034]), total cholesterol (P=.038 [CI: 1.004–1.164]), fibrosis grade (P<.001 [CI: 0.000–0.844]), and FokI (P=.028) allele T presence. Association was found between VDR polymorphism and fibrosis (BsmI andTaqI), triglycerides (TaqI), and HDL (FokI). DBP polymorphism was associated to HCV genotype (GC rs7041), previous HCV treatment, and GGT (GC rs4588). In conclusion, lowfrequency of vitamin D deficiencywas found, but VDR polymorphisms were frequently associated to fibrosis grade suggesting that they could be used as disease evaluation markers to understand the mechanisms underlying the virus–host interaction. |
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Scalioni, Letícia de PaulaSantos, Betânia Rodrigues dosSpritzer, Poli MaraVillela-Nogueira, Cristiane AlvesLewis Ximenez, Lia LauraPollo-Flores, PriscilaEsberard, Eliane Bordalo CathaláBrandão-Mello, Carlos E.Lampe, ElisabethVillar, Livia Melo2018-04-05T02:25:22Z20180025-7974http://hdl.handle.net/10183/174388001063002Potential relationship of vitamin D, vitamin D receptor (VDR), and vitamin D binding protein (DBP) have been suggested in the pathophysiology of hepatitis C virus (HCV) infection. The aim of this observational study is to determine vitamin D levels, and VDR and DBP genetic polymorphism according demographic and laboratory data in chronic HCV patients (CHC). A total of 148 CHC patients gave serum samples for testing 25-hydroxyvitamin D (25 (OH)D) level by immunochemiluminometric assay (<20ng/mL defined as deficient) and donated blood samples to allelic discrimination analysis using TaqMan assays. Analyzed single nucleotide polymorphisms (SNPs) were: VDR-rs7975232 (ApaI) C>A, rs731236 A>G (TaqI), rs1544410 C>T (BsmI), rs10735810 T>C (FokI) and carrier globulin/binding protein (GC)-rs4588 and rs7041 and the haplotype bAt [CCA]. Hepatic fibrosis was assessed using Fib-4 and Forns index. Eighty-two (54.40%) patients demonstrated deficiency of vitamin D and this was associated to AST (P=.019 [CI: 1.003–1.034]), total cholesterol (P=.038 [CI: 1.004–1.164]), fibrosis grade (P<.001 [CI: 0.000–0.844]), and FokI (P=.028) allele T presence. Association was found between VDR polymorphism and fibrosis (BsmI andTaqI), triglycerides (TaqI), and HDL (FokI). DBP polymorphism was associated to HCV genotype (GC rs7041), previous HCV treatment, and GGT (GC rs4588). In conclusion, lowfrequency of vitamin D deficiencywas found, but VDR polymorphisms were frequently associated to fibrosis grade suggesting that they could be used as disease evaluation markers to understand the mechanisms underlying the virus–host interaction.application/pdfengMedicine (Baltimore). Baltimore. Vol. 97, no. 8 (Feb. 2018), e9878 [7] p.FibroseHepatite CPolimorfismo genéticoVitamina DFibrosisHepatitis CPolymorphismVitamin DImpact of vitamin D receptor and binding protein gene polymorphisms in clinical and laboratory data of HCV patients : cross sectional studyEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001063002.pdf001063002.pdfTexto completo (inglês)application/pdf353966http://www.lume.ufrgs.br/bitstream/10183/174388/1/001063002.pdf1ba80e145962cec1c77daccdede2a623MD51TEXT001063002.pdf.txt001063002.pdf.txtExtracted Texttext/plain39819http://www.lume.ufrgs.br/bitstream/10183/174388/2/001063002.pdf.txt1a03625417f864b26548a63758dfaf61MD52THUMBNAIL001063002.pdf.jpg001063002.pdf.jpgGenerated Thumbnailimage/jpeg2015http://www.lume.ufrgs.br/bitstream/10183/174388/3/001063002.pdf.jpgb5ab6a8a69783149f9587bed60364dd9MD5310183/1743882018-10-25 10:10:21.296oai:www.lume.ufrgs.br:10183/174388Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-25T13:10:21Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Impact of vitamin D receptor and binding protein gene polymorphisms in clinical and laboratory data of HCV patients : cross sectional study |
title |
Impact of vitamin D receptor and binding protein gene polymorphisms in clinical and laboratory data of HCV patients : cross sectional study |
spellingShingle |
Impact of vitamin D receptor and binding protein gene polymorphisms in clinical and laboratory data of HCV patients : cross sectional study Scalioni, Letícia de Paula Fibrose Hepatite C Polimorfismo genético Vitamina D Fibrosis Hepatitis C Polymorphism Vitamin D |
title_short |
Impact of vitamin D receptor and binding protein gene polymorphisms in clinical and laboratory data of HCV patients : cross sectional study |
title_full |
Impact of vitamin D receptor and binding protein gene polymorphisms in clinical and laboratory data of HCV patients : cross sectional study |
title_fullStr |
Impact of vitamin D receptor and binding protein gene polymorphisms in clinical and laboratory data of HCV patients : cross sectional study |
title_full_unstemmed |
Impact of vitamin D receptor and binding protein gene polymorphisms in clinical and laboratory data of HCV patients : cross sectional study |
title_sort |
Impact of vitamin D receptor and binding protein gene polymorphisms in clinical and laboratory data of HCV patients : cross sectional study |
author |
Scalioni, Letícia de Paula |
author_facet |
Scalioni, Letícia de Paula Santos, Betânia Rodrigues dos Spritzer, Poli Mara Villela-Nogueira, Cristiane Alves Lewis Ximenez, Lia Laura Pollo-Flores, Priscila Esberard, Eliane Bordalo Cathalá Brandão-Mello, Carlos E. Lampe, Elisabeth Villar, Livia Melo |
author_role |
author |
author2 |
Santos, Betânia Rodrigues dos Spritzer, Poli Mara Villela-Nogueira, Cristiane Alves Lewis Ximenez, Lia Laura Pollo-Flores, Priscila Esberard, Eliane Bordalo Cathalá Brandão-Mello, Carlos E. Lampe, Elisabeth Villar, Livia Melo |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Scalioni, Letícia de Paula Santos, Betânia Rodrigues dos Spritzer, Poli Mara Villela-Nogueira, Cristiane Alves Lewis Ximenez, Lia Laura Pollo-Flores, Priscila Esberard, Eliane Bordalo Cathalá Brandão-Mello, Carlos E. Lampe, Elisabeth Villar, Livia Melo |
dc.subject.por.fl_str_mv |
Fibrose Hepatite C Polimorfismo genético Vitamina D |
topic |
Fibrose Hepatite C Polimorfismo genético Vitamina D Fibrosis Hepatitis C Polymorphism Vitamin D |
dc.subject.eng.fl_str_mv |
Fibrosis Hepatitis C Polymorphism Vitamin D |
description |
Potential relationship of vitamin D, vitamin D receptor (VDR), and vitamin D binding protein (DBP) have been suggested in the pathophysiology of hepatitis C virus (HCV) infection. The aim of this observational study is to determine vitamin D levels, and VDR and DBP genetic polymorphism according demographic and laboratory data in chronic HCV patients (CHC). A total of 148 CHC patients gave serum samples for testing 25-hydroxyvitamin D (25 (OH)D) level by immunochemiluminometric assay (<20ng/mL defined as deficient) and donated blood samples to allelic discrimination analysis using TaqMan assays. Analyzed single nucleotide polymorphisms (SNPs) were: VDR-rs7975232 (ApaI) C>A, rs731236 A>G (TaqI), rs1544410 C>T (BsmI), rs10735810 T>C (FokI) and carrier globulin/binding protein (GC)-rs4588 and rs7041 and the haplotype bAt [CCA]. Hepatic fibrosis was assessed using Fib-4 and Forns index. Eighty-two (54.40%) patients demonstrated deficiency of vitamin D and this was associated to AST (P=.019 [CI: 1.003–1.034]), total cholesterol (P=.038 [CI: 1.004–1.164]), fibrosis grade (P<.001 [CI: 0.000–0.844]), and FokI (P=.028) allele T presence. Association was found between VDR polymorphism and fibrosis (BsmI andTaqI), triglycerides (TaqI), and HDL (FokI). DBP polymorphism was associated to HCV genotype (GC rs7041), previous HCV treatment, and GGT (GC rs4588). In conclusion, lowfrequency of vitamin D deficiencywas found, but VDR polymorphisms were frequently associated to fibrosis grade suggesting that they could be used as disease evaluation markers to understand the mechanisms underlying the virus–host interaction. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-04-05T02:25:22Z |
dc.date.issued.fl_str_mv |
2018 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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0025-7974 |
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001063002 |
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http://hdl.handle.net/10183/174388 |
dc.language.iso.fl_str_mv |
eng |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Medicine (Baltimore). Baltimore. Vol. 97, no. 8 (Feb. 2018), e9878 [7] p. |
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