Establishment of a pathogenicity index in salmonella enteritidis and salmonella typhimurium strains inoculated in one-day-old broiler chicks
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/150125 |
Resumo: | Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 106 CFU, was inoculated in 10 one-day-old broilers. Mortality after inoculation, time of death (TD), and the presence of six macroscopic lesions were evaluated over a period of seven days post-inoculation (dpi). A Pathogenicity Index Per Bird (IPI), ranging 0 to 10, was calculated. Liver and heart fragments were collected to reisolate the bacteria. Blood was collected from the surviving birds, and an ELISA test was carried out to detect specific antibodies. The median of the pathogenicity indices, the number of lesions and the rate of bacteria reisolation were significantly different (p<0.05) among the origins of the isolates (p<0.05). The pathogenicity index developed in this study allows the classification of Pasteurella multocida pathogenicity and may be an alternative to the pathogenicity models currently used for screening. |
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Lima, Daiane Alves deFurian, Thales QuediPilatti, Roberta MarmittSilva, G.L.Morgam, R.B.Borges, Karen ApellanisFortes, F.B.B.Moraes, Hamilton Luiz de SouzaBrito, Benito Guimarães deBrito, Karen Krystine Gonçalves deSalle, Carlos Tadeu Pippi2016-12-16T02:16:57Z20160102-0935http://hdl.handle.net/10183/150125000999610Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 106 CFU, was inoculated in 10 one-day-old broilers. Mortality after inoculation, time of death (TD), and the presence of six macroscopic lesions were evaluated over a period of seven days post-inoculation (dpi). A Pathogenicity Index Per Bird (IPI), ranging 0 to 10, was calculated. Liver and heart fragments were collected to reisolate the bacteria. Blood was collected from the surviving birds, and an ELISA test was carried out to detect specific antibodies. The median of the pathogenicity indices, the number of lesions and the rate of bacteria reisolation were significantly different (p<0.05) among the origins of the isolates (p<0.05). The pathogenicity index developed in this study allows the classification of Pasteurella multocida pathogenicity and may be an alternative to the pathogenicity models currently used for screening.application/pdfporArquivo brasileiro de medicina veterinaria e zootecnia= Brazilian journal of veterinary and animal sciences. Belo Horizonte. Vol. 68, n.2 (2016), p. 257-264Patologia aviária : SalmonellaVirulênciaPatogenicidadeAvesSalmonella typhimuriumSalmonella enteritidisFowl choleraInoculationPasteurella multocidaPathogenicity indexEstablishment of a pathogenicity index in salmonella enteritidis and salmonella typhimurium strains inoculated in one-day-old broiler chicksEstabelecimento de um índice de patogenicidade em cepas de salmonella enteritidis e salmonella typhimurium inoculadas em pintos de um dia de idade info:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000999610.pdf000999610.pdfTexto completoapplication/pdf109470http://www.lume.ufrgs.br/bitstream/10183/150125/1/000999610.pdfa08fcca12b4424651dddff2d6218a3f0MD51TEXT000999610.pdf.txt000999610.pdf.txtExtracted Texttext/plain26566http://www.lume.ufrgs.br/bitstream/10183/150125/2/000999610.pdf.txta7d3a21d91bda51d9ee5c48584d91c09MD52THUMBNAIL000999610.pdf.jpg000999610.pdf.jpgGenerated Thumbnailimage/jpeg2082http://www.lume.ufrgs.br/bitstream/10183/150125/3/000999610.pdf.jpg388043462fa89dd1e2743f392709bb54MD5310183/1501252018-10-30 07:54:22.053oai:www.lume.ufrgs.br:10183/150125Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-30T10:54:22Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Establishment of a pathogenicity index in salmonella enteritidis and salmonella typhimurium strains inoculated in one-day-old broiler chicks |
dc.title.alternative.pt_BR.fl_str_mv |
Estabelecimento de um índice de patogenicidade em cepas de salmonella enteritidis e salmonella typhimurium inoculadas em pintos de um dia de idade |
title |
Establishment of a pathogenicity index in salmonella enteritidis and salmonella typhimurium strains inoculated in one-day-old broiler chicks |
spellingShingle |
Establishment of a pathogenicity index in salmonella enteritidis and salmonella typhimurium strains inoculated in one-day-old broiler chicks Lima, Daiane Alves de Patologia aviária : Salmonella Virulência Patogenicidade Aves Salmonella typhimurium Salmonella enteritidis Fowl cholera Inoculation Pasteurella multocida Pathogenicity index |
title_short |
Establishment of a pathogenicity index in salmonella enteritidis and salmonella typhimurium strains inoculated in one-day-old broiler chicks |
title_full |
Establishment of a pathogenicity index in salmonella enteritidis and salmonella typhimurium strains inoculated in one-day-old broiler chicks |
title_fullStr |
Establishment of a pathogenicity index in salmonella enteritidis and salmonella typhimurium strains inoculated in one-day-old broiler chicks |
title_full_unstemmed |
Establishment of a pathogenicity index in salmonella enteritidis and salmonella typhimurium strains inoculated in one-day-old broiler chicks |
title_sort |
Establishment of a pathogenicity index in salmonella enteritidis and salmonella typhimurium strains inoculated in one-day-old broiler chicks |
author |
Lima, Daiane Alves de |
author_facet |
Lima, Daiane Alves de Furian, Thales Quedi Pilatti, Roberta Marmitt Silva, G.L. Morgam, R.B. Borges, Karen Apellanis Fortes, F.B.B. Moraes, Hamilton Luiz de Souza Brito, Benito Guimarães de Brito, Karen Krystine Gonçalves de Salle, Carlos Tadeu Pippi |
author_role |
author |
author2 |
Furian, Thales Quedi Pilatti, Roberta Marmitt Silva, G.L. Morgam, R.B. Borges, Karen Apellanis Fortes, F.B.B. Moraes, Hamilton Luiz de Souza Brito, Benito Guimarães de Brito, Karen Krystine Gonçalves de Salle, Carlos Tadeu Pippi |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lima, Daiane Alves de Furian, Thales Quedi Pilatti, Roberta Marmitt Silva, G.L. Morgam, R.B. Borges, Karen Apellanis Fortes, F.B.B. Moraes, Hamilton Luiz de Souza Brito, Benito Guimarães de Brito, Karen Krystine Gonçalves de Salle, Carlos Tadeu Pippi |
dc.subject.por.fl_str_mv |
Patologia aviária : Salmonella Virulência Patogenicidade Aves Salmonella typhimurium Salmonella enteritidis |
topic |
Patologia aviária : Salmonella Virulência Patogenicidade Aves Salmonella typhimurium Salmonella enteritidis Fowl cholera Inoculation Pasteurella multocida Pathogenicity index |
dc.subject.eng.fl_str_mv |
Fowl cholera Inoculation Pasteurella multocida Pathogenicity index |
description |
Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 106 CFU, was inoculated in 10 one-day-old broilers. Mortality after inoculation, time of death (TD), and the presence of six macroscopic lesions were evaluated over a period of seven days post-inoculation (dpi). A Pathogenicity Index Per Bird (IPI), ranging 0 to 10, was calculated. Liver and heart fragments were collected to reisolate the bacteria. Blood was collected from the surviving birds, and an ELISA test was carried out to detect specific antibodies. The median of the pathogenicity indices, the number of lesions and the rate of bacteria reisolation were significantly different (p<0.05) among the origins of the isolates (p<0.05). The pathogenicity index developed in this study allows the classification of Pasteurella multocida pathogenicity and may be an alternative to the pathogenicity models currently used for screening. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-12-16T02:16:57Z |
dc.date.issued.fl_str_mv |
2016 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/150125 |
dc.identifier.issn.pt_BR.fl_str_mv |
0102-0935 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000999610 |
identifier_str_mv |
0102-0935 000999610 |
url |
http://hdl.handle.net/10183/150125 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Arquivo brasileiro de medicina veterinaria e zootecnia= Brazilian journal of veterinary and animal sciences. Belo Horizonte. Vol. 68, n.2 (2016), p. 257-264 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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