DNA Isolation from Fresh and Cryopreserved Semen of Tambaqui (Colossoma macropomum)

Detalhes bibliográficos
Autor(a) principal: Mello, Fernanda de
Data de Publicação: 2016
Outros Autores: Garcia, Juliana Saraiva, Depincé, Alexandra, Labbé, Catherine, Streit Júnior, Danilo Pedro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/204427
Resumo: Semen cryopreservation is an import toll for reproductive management of fish species, which increasingly it is applied in the commercial production of fish. Cryopreservation need of the cryoprotectors agents (CPAs) that may be a complication factor in DNA extraction that can affect the quality of isolated DNA. Genomic DNA constitutes the total genetic information of an organism and it is used for a many finalities, such as to determine gene structure or to detect the presence or absence of genes of interest. However, simple questions to be analyzed become slow to be answered due to troubles in the execution of basic techniques like DNA isolation. This article describes a protocol for the isolation of genomic DNA from fresh and cryopreserved semen.
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spelling Mello, Fernanda deGarcia, Juliana SaraivaDepincé, AlexandraLabbé, CatherineStreit Júnior, Danilo Pedro2020-01-17T04:09:47Z20161307-234Xhttp://hdl.handle.net/10183/204427001107847Semen cryopreservation is an import toll for reproductive management of fish species, which increasingly it is applied in the commercial production of fish. Cryopreservation need of the cryoprotectors agents (CPAs) that may be a complication factor in DNA extraction that can affect the quality of isolated DNA. Genomic DNA constitutes the total genetic information of an organism and it is used for a many finalities, such as to determine gene structure or to detect the presence or absence of genes of interest. However, simple questions to be analyzed become slow to be answered due to troubles in the execution of basic techniques like DNA isolation. This article describes a protocol for the isolation of genomic DNA from fresh and cryopreserved semen.application/pdfengJournal of FisheriesSciences.com. London. Vol. 10, n. 2 (2016), p. 21-24CriopreservaçãoSêmenReprodução animalGenética animalAquacultureDNA ExtractionCryopreservationEpigenetics AlterationSperm CellsDNA Isolation from Fresh and Cryopreserved Semen of Tambaqui (Colossoma macropomum)Estrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001107847.pdf.txt001107847.pdf.txtExtracted Texttext/plain14072http://www.lume.ufrgs.br/bitstream/10183/204427/2/001107847.pdf.txtb8c2b90abe3da915c5d0988b3f1620f0MD52ORIGINAL001107847.pdfTexto completo (inglês)application/pdf570772http://www.lume.ufrgs.br/bitstream/10183/204427/1/001107847.pdfde58a2bbe653e11f0c3c53f133f939efMD5110183/2044272020-01-18 05:14:58.923278oai:www.lume.ufrgs.br:10183/204427Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2020-01-18T07:14:58Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv DNA Isolation from Fresh and Cryopreserved Semen of Tambaqui (Colossoma macropomum)
title DNA Isolation from Fresh and Cryopreserved Semen of Tambaqui (Colossoma macropomum)
spellingShingle DNA Isolation from Fresh and Cryopreserved Semen of Tambaqui (Colossoma macropomum)
Mello, Fernanda de
Criopreservação
Sêmen
Reprodução animal
Genética animal
Aquaculture
DNA Extraction
Cryopreservation
Epigenetics Alteration
Sperm Cells
title_short DNA Isolation from Fresh and Cryopreserved Semen of Tambaqui (Colossoma macropomum)
title_full DNA Isolation from Fresh and Cryopreserved Semen of Tambaqui (Colossoma macropomum)
title_fullStr DNA Isolation from Fresh and Cryopreserved Semen of Tambaqui (Colossoma macropomum)
title_full_unstemmed DNA Isolation from Fresh and Cryopreserved Semen of Tambaqui (Colossoma macropomum)
title_sort DNA Isolation from Fresh and Cryopreserved Semen of Tambaqui (Colossoma macropomum)
author Mello, Fernanda de
author_facet Mello, Fernanda de
Garcia, Juliana Saraiva
Depincé, Alexandra
Labbé, Catherine
Streit Júnior, Danilo Pedro
author_role author
author2 Garcia, Juliana Saraiva
Depincé, Alexandra
Labbé, Catherine
Streit Júnior, Danilo Pedro
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mello, Fernanda de
Garcia, Juliana Saraiva
Depincé, Alexandra
Labbé, Catherine
Streit Júnior, Danilo Pedro
dc.subject.por.fl_str_mv Criopreservação
Sêmen
Reprodução animal
Genética animal
topic Criopreservação
Sêmen
Reprodução animal
Genética animal
Aquaculture
DNA Extraction
Cryopreservation
Epigenetics Alteration
Sperm Cells
dc.subject.eng.fl_str_mv Aquaculture
DNA Extraction
Cryopreservation
Epigenetics Alteration
Sperm Cells
description Semen cryopreservation is an import toll for reproductive management of fish species, which increasingly it is applied in the commercial production of fish. Cryopreservation need of the cryoprotectors agents (CPAs) that may be a complication factor in DNA extraction that can affect the quality of isolated DNA. Genomic DNA constitutes the total genetic information of an organism and it is used for a many finalities, such as to determine gene structure or to detect the presence or absence of genes of interest. However, simple questions to be analyzed become slow to be answered due to troubles in the execution of basic techniques like DNA isolation. This article describes a protocol for the isolation of genomic DNA from fresh and cryopreserved semen.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2020-01-17T04:09:47Z
dc.type.driver.fl_str_mv Estrangeiro
info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/204427
dc.identifier.issn.pt_BR.fl_str_mv 1307-234X
dc.identifier.nrb.pt_BR.fl_str_mv 001107847
identifier_str_mv 1307-234X
001107847
url http://hdl.handle.net/10183/204427
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Journal of FisheriesSciences.com. London. Vol. 10, n. 2 (2016), p. 21-24
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
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collection Repositório Institucional da UFRGS
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