Bovine herpesvirus 4 in Parana state, Brazil : case report, viral isolation, and molecular identification

Detalhes bibliográficos
Autor(a) principal: Kruger, Ernesto Renato
Data de Publicação: 2015
Outros Autores: Penha, Tania Regina, Stoffelo, Daura Regina Eira, Roehe, Paulo Michel, Ribeiro, Magda Clara Vieira da Costa, Soccol, Vanete Thomaz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/225948
Resumo: Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinae sub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical capsid symmetry surrounded by a loose envelope, typical of a herpesvirus. A 2,571 bp PCR product after HindIII digestion generated four fragments, whose base pair composition were 403, 420, 535, and 1,125 bp. Restriction enzymes HindIII and BamHI generated the expected diagnostic bands as well as a 2,350 bp hypermolar fragment as a result of BamHI treatment to demonstrate that agent was a bovine herpesvirus 4, appertaining to DN-599 group.
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spelling Kruger, Ernesto RenatoPenha, Tania ReginaStoffelo, Daura Regina EiraRoehe, Paulo MichelRibeiro, Magda Clara Vieira da CostaSoccol, Vanete Thomaz2021-08-20T04:10:08Z20151517-8382http://hdl.handle.net/10183/225948000996374Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinae sub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical capsid symmetry surrounded by a loose envelope, typical of a herpesvirus. A 2,571 bp PCR product after HindIII digestion generated four fragments, whose base pair composition were 403, 420, 535, and 1,125 bp. Restriction enzymes HindIII and BamHI generated the expected diagnostic bands as well as a 2,350 bp hypermolar fragment as a result of BamHI treatment to demonstrate that agent was a bovine herpesvirus 4, appertaining to DN-599 group.application/pdfengBrazilian journal of microbiology. São Paulo. Vol. 46, no. 1 (Jan./Mar. 2015), p. 279-283Herpesvirus bovino 4Reação em cadeia da polimerasePolimorfismo de fragmento de restriçãoDermatoglifiaBoHV-4 virusFingerprintBovine herpesvirus 4 in Parana state, Brazil : case report, viral isolation, and molecular identificationinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000996374.pdf.txt000996374.pdf.txtExtracted Texttext/plain20606http://www.lume.ufrgs.br/bitstream/10183/225948/2/000996374.pdf.txt90916ae72ec581b0c2d07617b311f816MD52ORIGINAL000996374.pdfTexto completo (inglês)application/pdf1733062http://www.lume.ufrgs.br/bitstream/10183/225948/1/000996374.pdf98948e8ce976991238578c0f907e3a82MD5110183/2259482021-09-18 04:43:26.776421oai:www.lume.ufrgs.br:10183/225948Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-09-18T07:43:26Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Bovine herpesvirus 4 in Parana state, Brazil : case report, viral isolation, and molecular identification
title Bovine herpesvirus 4 in Parana state, Brazil : case report, viral isolation, and molecular identification
spellingShingle Bovine herpesvirus 4 in Parana state, Brazil : case report, viral isolation, and molecular identification
Kruger, Ernesto Renato
Herpesvirus bovino 4
Reação em cadeia da polimerase
Polimorfismo de fragmento de restrição
Dermatoglifia
BoHV-4 virus
Fingerprint
title_short Bovine herpesvirus 4 in Parana state, Brazil : case report, viral isolation, and molecular identification
title_full Bovine herpesvirus 4 in Parana state, Brazil : case report, viral isolation, and molecular identification
title_fullStr Bovine herpesvirus 4 in Parana state, Brazil : case report, viral isolation, and molecular identification
title_full_unstemmed Bovine herpesvirus 4 in Parana state, Brazil : case report, viral isolation, and molecular identification
title_sort Bovine herpesvirus 4 in Parana state, Brazil : case report, viral isolation, and molecular identification
author Kruger, Ernesto Renato
author_facet Kruger, Ernesto Renato
Penha, Tania Regina
Stoffelo, Daura Regina Eira
Roehe, Paulo Michel
Ribeiro, Magda Clara Vieira da Costa
Soccol, Vanete Thomaz
author_role author
author2 Penha, Tania Regina
Stoffelo, Daura Regina Eira
Roehe, Paulo Michel
Ribeiro, Magda Clara Vieira da Costa
Soccol, Vanete Thomaz
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Kruger, Ernesto Renato
Penha, Tania Regina
Stoffelo, Daura Regina Eira
Roehe, Paulo Michel
Ribeiro, Magda Clara Vieira da Costa
Soccol, Vanete Thomaz
dc.subject.por.fl_str_mv Herpesvirus bovino 4
Reação em cadeia da polimerase
Polimorfismo de fragmento de restrição
Dermatoglifia
topic Herpesvirus bovino 4
Reação em cadeia da polimerase
Polimorfismo de fragmento de restrição
Dermatoglifia
BoHV-4 virus
Fingerprint
dc.subject.eng.fl_str_mv BoHV-4 virus
Fingerprint
description Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinae sub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical capsid symmetry surrounded by a loose envelope, typical of a herpesvirus. A 2,571 bp PCR product after HindIII digestion generated four fragments, whose base pair composition were 403, 420, 535, and 1,125 bp. Restriction enzymes HindIII and BamHI generated the expected diagnostic bands as well as a 2,350 bp hypermolar fragment as a result of BamHI treatment to demonstrate that agent was a bovine herpesvirus 4, appertaining to DN-599 group.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2021-08-20T04:10:08Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/225948
dc.identifier.issn.pt_BR.fl_str_mv 1517-8382
dc.identifier.nrb.pt_BR.fl_str_mv 000996374
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dc.relation.ispartof.pt_BR.fl_str_mv Brazilian journal of microbiology. São Paulo. Vol. 46, no. 1 (Jan./Mar. 2015), p. 279-283
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reponame_str Repositório Institucional da UFRGS
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