Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices
Autor(a) principal: | |
---|---|
Data de Publicação: | 2001 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/21133 |
Resumo: | It has been suggested that glucocorticoids released during stress might impair neuronal function by decreasing glucose uptake by hippocampal neurons. Previous work has demonstrated that glucose uptake is reduced in hippocampal and cerebral cortex slices 24 h after exposure to acute stress, while no effect was observed after repeated stress. Here, we report the effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices and on plasma glucose and corticosterone levels. Male adult Wistar rats were exposed to restraint 1 h/day for 50 days in the chronic model. In the acute model there was a single exposure. Immediately or 24 h after stress, the animals were sacrificed and the hippocampus and cerebral cortex were dissected, sliced, and incubated with Krebs buffer, pH 7.4, containing 5 mM glucose and 0.2 µCi D-[U-14C] glucose. CO2 production from glucose was estimated. Trunk blood was also collected, and both corticosterone and glucose were measured. The results showed that corticosterone levels after exposure to acute restraint were increased, but the increase was smaller when the animals were submitted to repeated stress. Blood glucose levels increased after both acute and repeated stress. However, glucose utilization, measured as CO2 production in hippocampal and cerebral cortex slices, was the same in stressed and control groups under conditions of both acute and chronic stress. We conclude that, although stress may induce a decrease in glucose uptake, this effect is not sufficient to affect the energy metabolism of these cells. |
id |
UFRGS-2_75b2ef9394d544abd8e125e255b20e4f |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/21133 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Torres, Iraci Lucena da SilvaGamaro, Giovana DuzzoCucco, Simone Nascimento SilveiraMichalowski, Mariana BohnsCorrea, Jaqueline Betina BroenstrupPerry, Marcos Luiz SantosDalmaz, Carla2010-04-24T04:15:27Z20010100-879Xhttp://hdl.handle.net/10183/21133000326907It has been suggested that glucocorticoids released during stress might impair neuronal function by decreasing glucose uptake by hippocampal neurons. Previous work has demonstrated that glucose uptake is reduced in hippocampal and cerebral cortex slices 24 h after exposure to acute stress, while no effect was observed after repeated stress. Here, we report the effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices and on plasma glucose and corticosterone levels. Male adult Wistar rats were exposed to restraint 1 h/day for 50 days in the chronic model. In the acute model there was a single exposure. Immediately or 24 h after stress, the animals were sacrificed and the hippocampus and cerebral cortex were dissected, sliced, and incubated with Krebs buffer, pH 7.4, containing 5 mM glucose and 0.2 µCi D-[U-14C] glucose. CO2 production from glucose was estimated. Trunk blood was also collected, and both corticosterone and glucose were measured. The results showed that corticosterone levels after exposure to acute restraint were increased, but the increase was smaller when the animals were submitted to repeated stress. Blood glucose levels increased after both acute and repeated stress. However, glucose utilization, measured as CO2 production in hippocampal and cerebral cortex slices, was the same in stressed and control groups under conditions of both acute and chronic stress. We conclude that, although stress may induce a decrease in glucose uptake, this effect is not sufficient to affect the energy metabolism of these cells.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 1 (Jan. 2001), p. 111-116BioquímicaRestraint stressChronic stressCO2 productionGlucose oxidationHippocampusCerebral cortexEffect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slicesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000326907.pdf000326907.pdfTexto completo (inglês)application/pdf102106http://www.lume.ufrgs.br/bitstream/10183/21133/1/000326907.pdf65818a6d16fad119dce78f20b7d63d67MD51TEXT000326907.pdf.txt000326907.pdf.txtExtracted Texttext/plain21232http://www.lume.ufrgs.br/bitstream/10183/21133/2/000326907.pdf.txt3aaa22c0ff376d69bfb77f06a1c39c9bMD52THUMBNAIL000326907.pdf.jpg000326907.pdf.jpgGenerated Thumbnailimage/jpeg1664http://www.lume.ufrgs.br/bitstream/10183/21133/3/000326907.pdf.jpg0992eab26ed9d146c13e001dfa00ca08MD5310183/211332021-11-20 06:12:17.90942oai:www.lume.ufrgs.br:10183/21133Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-11-20T08:12:17Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices |
title |
Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices |
spellingShingle |
Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices Torres, Iraci Lucena da Silva Bioquímica Restraint stress Chronic stress CO2 production Glucose oxidation Hippocampus Cerebral cortex |
title_short |
Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices |
title_full |
Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices |
title_fullStr |
Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices |
title_full_unstemmed |
Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices |
title_sort |
Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices |
author |
Torres, Iraci Lucena da Silva |
author_facet |
Torres, Iraci Lucena da Silva Gamaro, Giovana Duzzo Cucco, Simone Nascimento Silveira Michalowski, Mariana Bohns Correa, Jaqueline Betina Broenstrup Perry, Marcos Luiz Santos Dalmaz, Carla |
author_role |
author |
author2 |
Gamaro, Giovana Duzzo Cucco, Simone Nascimento Silveira Michalowski, Mariana Bohns Correa, Jaqueline Betina Broenstrup Perry, Marcos Luiz Santos Dalmaz, Carla |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Torres, Iraci Lucena da Silva Gamaro, Giovana Duzzo Cucco, Simone Nascimento Silveira Michalowski, Mariana Bohns Correa, Jaqueline Betina Broenstrup Perry, Marcos Luiz Santos Dalmaz, Carla |
dc.subject.por.fl_str_mv |
Bioquímica |
topic |
Bioquímica Restraint stress Chronic stress CO2 production Glucose oxidation Hippocampus Cerebral cortex |
dc.subject.eng.fl_str_mv |
Restraint stress Chronic stress CO2 production Glucose oxidation Hippocampus Cerebral cortex |
description |
It has been suggested that glucocorticoids released during stress might impair neuronal function by decreasing glucose uptake by hippocampal neurons. Previous work has demonstrated that glucose uptake is reduced in hippocampal and cerebral cortex slices 24 h after exposure to acute stress, while no effect was observed after repeated stress. Here, we report the effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices and on plasma glucose and corticosterone levels. Male adult Wistar rats were exposed to restraint 1 h/day for 50 days in the chronic model. In the acute model there was a single exposure. Immediately or 24 h after stress, the animals were sacrificed and the hippocampus and cerebral cortex were dissected, sliced, and incubated with Krebs buffer, pH 7.4, containing 5 mM glucose and 0.2 µCi D-[U-14C] glucose. CO2 production from glucose was estimated. Trunk blood was also collected, and both corticosterone and glucose were measured. The results showed that corticosterone levels after exposure to acute restraint were increased, but the increase was smaller when the animals were submitted to repeated stress. Blood glucose levels increased after both acute and repeated stress. However, glucose utilization, measured as CO2 production in hippocampal and cerebral cortex slices, was the same in stressed and control groups under conditions of both acute and chronic stress. We conclude that, although stress may induce a decrease in glucose uptake, this effect is not sufficient to affect the energy metabolism of these cells. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001 |
dc.date.accessioned.fl_str_mv |
2010-04-24T04:15:27Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/21133 |
dc.identifier.issn.pt_BR.fl_str_mv |
0100-879X |
dc.identifier.nrb.pt_BR.fl_str_mv |
000326907 |
identifier_str_mv |
0100-879X 000326907 |
url |
http://hdl.handle.net/10183/21133 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 1 (Jan. 2001), p. 111-116 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/21133/1/000326907.pdf http://www.lume.ufrgs.br/bitstream/10183/21133/2/000326907.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/21133/3/000326907.pdf.jpg |
bitstream.checksum.fl_str_mv |
65818a6d16fad119dce78f20b7d63d67 3aaa22c0ff376d69bfb77f06a1c39c9b 0992eab26ed9d146c13e001dfa00ca08 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1792790152991473664 |