New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/110154 |
Resumo: | Background: Mycoplasma hyopneumoniae, Mycoplasma flocculare and Mycoplasma hyorhinis live in swine respiratory tracts. M. flocculare, a commensal bacterium, is genetically closely related to M. hyopneumoniae, the causative agent of enzootic porcine pneumonia. M. hyorhinis is also pathogenic, causing polyserositis and arthritis. In this work, we present the genome sequences of M. flocculare and M. hyopneumoniae strain 7422, and we compare these genomes with the genomes of other M. hyoponeumoniae strain and to the a M. hyorhinis genome. These analyses were performed to identify possible characteristics that may help to explain the different behaviors of these species in swine respiratory tracts. Results: The overall genome organization of three species was analyzed, revealing that the ORF clusters (OCs) differ considerably and that inversions and rearrangements are common. Although M. flocculare and M. hyopneumoniae display a high degree of similarity with respect to the gene content, only some genomic regions display considerable synteny. Genes encoding proteins that may be involved in host-cell adhesion in M. hyopneumoniae and M. flocculare display differences in genomic structure and organization. Some genes encoding adhesins of the P97 family are absent in M. flocculare and some contain sequence differences or lack of domains that are considered to be important for adhesion to host cells. The phylogenetic relationship of the three species was confirmed by a phylogenomic approach. The set of genes involved in metabolism, especially in the uptake of precursors for nucleic acids synthesis and nucleotide metabolism, display some differences in copy number and the presence/absence in the three species. Conclusions: The comparative analyses of three mycoplasma species that inhabit the swine respiratory tract facilitated the identification of some characteristics that may be related to their different behaviors. M. hyopneumoniae and M. flocculare display many differences that may help to explain why one species is pathogenic and the other is considered to be commensal. However, it was not possible to identify specific virulence determinant factors that could explain the differences in the pathogenicity of the analyzed species. The M. hyorhinis genome contains differences in some components involved in metabolism and evasion of the host’s immune system that may contribute to its growth aggressiveness. Several horizontal gene transfer events were identified. The phylogenomic analysis places M. hyopneumoniae, M. flocculare and M. hyorhinis in the hyopneumoniae clade. |
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Siqueira, Franciele MaboniThompson, Claudia ElizabethVirginio, Veridiana GomesGonchoroski, TaylorReolon, Luciano AntonioAlmeida, Luiz Gonzaga Paula deFonseca, Marbella Maria BernardesSouza, Rangel CelsoProsdocimi, FranciscoSchrank, Irene SilveiraFerreira, Henrique BunselmeyerVasconcelos, Ana Tereza Ribeiro deZaha, Arnaldo2015-02-14T02:19:12Z2013http://hdl.handle.net/10183/110154000922240Background: Mycoplasma hyopneumoniae, Mycoplasma flocculare and Mycoplasma hyorhinis live in swine respiratory tracts. M. flocculare, a commensal bacterium, is genetically closely related to M. hyopneumoniae, the causative agent of enzootic porcine pneumonia. M. hyorhinis is also pathogenic, causing polyserositis and arthritis. In this work, we present the genome sequences of M. flocculare and M. hyopneumoniae strain 7422, and we compare these genomes with the genomes of other M. hyoponeumoniae strain and to the a M. hyorhinis genome. These analyses were performed to identify possible characteristics that may help to explain the different behaviors of these species in swine respiratory tracts. Results: The overall genome organization of three species was analyzed, revealing that the ORF clusters (OCs) differ considerably and that inversions and rearrangements are common. Although M. flocculare and M. hyopneumoniae display a high degree of similarity with respect to the gene content, only some genomic regions display considerable synteny. Genes encoding proteins that may be involved in host-cell adhesion in M. hyopneumoniae and M. flocculare display differences in genomic structure and organization. Some genes encoding adhesins of the P97 family are absent in M. flocculare and some contain sequence differences or lack of domains that are considered to be important for adhesion to host cells. The phylogenetic relationship of the three species was confirmed by a phylogenomic approach. The set of genes involved in metabolism, especially in the uptake of precursors for nucleic acids synthesis and nucleotide metabolism, display some differences in copy number and the presence/absence in the three species. Conclusions: The comparative analyses of three mycoplasma species that inhabit the swine respiratory tract facilitated the identification of some characteristics that may be related to their different behaviors. M. hyopneumoniae and M. flocculare display many differences that may help to explain why one species is pathogenic and the other is considered to be commensal. However, it was not possible to identify specific virulence determinant factors that could explain the differences in the pathogenicity of the analyzed species. The M. hyorhinis genome contains differences in some components involved in metabolism and evasion of the host’s immune system that may contribute to its growth aggressiveness. Several horizontal gene transfer events were identified. The phylogenomic analysis places M. hyopneumoniae, M. flocculare and M. hyorhinis in the hyopneumoniae clade.application/pdfengBMC Genomics. London. Vol. 14,(Mar. 2013), e175, 17 p.MicoplasmaTrato respiratórioSuínosMycoplasmaComparative genomicsAdhesinsSwine respiratory tractNew insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysisEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000922240.pdf000922240.pdfTexto completo (inglês)application/pdf553837http://www.lume.ufrgs.br/bitstream/10183/110154/1/000922240.pdf4ee93f9d4e630cbbb1380c758f158a94MD51TEXT000922240.pdf.txt000922240.pdf.txtExtracted Texttext/plain93748http://www.lume.ufrgs.br/bitstream/10183/110154/2/000922240.pdf.txtd61a3b98320006163d03f1b1570388f5MD52THUMBNAIL000922240.pdf.jpg000922240.pdf.jpgGenerated Thumbnailimage/jpeg1825http://www.lume.ufrgs.br/bitstream/10183/110154/3/000922240.pdf.jpg0304af7ceebffb8a9da0524a95bfa552MD5310183/1101542023-07-06 03:53:24.270063oai:www.lume.ufrgs.br:10183/110154Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-07-06T06:53:24Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis |
title |
New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis |
spellingShingle |
New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis Siqueira, Franciele Maboni Micoplasma Trato respiratório Suínos Mycoplasma Comparative genomics Adhesins Swine respiratory tract |
title_short |
New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis |
title_full |
New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis |
title_fullStr |
New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis |
title_full_unstemmed |
New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis |
title_sort |
New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis |
author |
Siqueira, Franciele Maboni |
author_facet |
Siqueira, Franciele Maboni Thompson, Claudia Elizabeth Virginio, Veridiana Gomes Gonchoroski, Taylor Reolon, Luciano Antonio Almeida, Luiz Gonzaga Paula de Fonseca, Marbella Maria Bernardes Souza, Rangel Celso Prosdocimi, Francisco Schrank, Irene Silveira Ferreira, Henrique Bunselmeyer Vasconcelos, Ana Tereza Ribeiro de Zaha, Arnaldo |
author_role |
author |
author2 |
Thompson, Claudia Elizabeth Virginio, Veridiana Gomes Gonchoroski, Taylor Reolon, Luciano Antonio Almeida, Luiz Gonzaga Paula de Fonseca, Marbella Maria Bernardes Souza, Rangel Celso Prosdocimi, Francisco Schrank, Irene Silveira Ferreira, Henrique Bunselmeyer Vasconcelos, Ana Tereza Ribeiro de Zaha, Arnaldo |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Siqueira, Franciele Maboni Thompson, Claudia Elizabeth Virginio, Veridiana Gomes Gonchoroski, Taylor Reolon, Luciano Antonio Almeida, Luiz Gonzaga Paula de Fonseca, Marbella Maria Bernardes Souza, Rangel Celso Prosdocimi, Francisco Schrank, Irene Silveira Ferreira, Henrique Bunselmeyer Vasconcelos, Ana Tereza Ribeiro de Zaha, Arnaldo |
dc.subject.por.fl_str_mv |
Micoplasma Trato respiratório Suínos |
topic |
Micoplasma Trato respiratório Suínos Mycoplasma Comparative genomics Adhesins Swine respiratory tract |
dc.subject.eng.fl_str_mv |
Mycoplasma Comparative genomics Adhesins Swine respiratory tract |
description |
Background: Mycoplasma hyopneumoniae, Mycoplasma flocculare and Mycoplasma hyorhinis live in swine respiratory tracts. M. flocculare, a commensal bacterium, is genetically closely related to M. hyopneumoniae, the causative agent of enzootic porcine pneumonia. M. hyorhinis is also pathogenic, causing polyserositis and arthritis. In this work, we present the genome sequences of M. flocculare and M. hyopneumoniae strain 7422, and we compare these genomes with the genomes of other M. hyoponeumoniae strain and to the a M. hyorhinis genome. These analyses were performed to identify possible characteristics that may help to explain the different behaviors of these species in swine respiratory tracts. Results: The overall genome organization of three species was analyzed, revealing that the ORF clusters (OCs) differ considerably and that inversions and rearrangements are common. Although M. flocculare and M. hyopneumoniae display a high degree of similarity with respect to the gene content, only some genomic regions display considerable synteny. Genes encoding proteins that may be involved in host-cell adhesion in M. hyopneumoniae and M. flocculare display differences in genomic structure and organization. Some genes encoding adhesins of the P97 family are absent in M. flocculare and some contain sequence differences or lack of domains that are considered to be important for adhesion to host cells. The phylogenetic relationship of the three species was confirmed by a phylogenomic approach. The set of genes involved in metabolism, especially in the uptake of precursors for nucleic acids synthesis and nucleotide metabolism, display some differences in copy number and the presence/absence in the three species. Conclusions: The comparative analyses of three mycoplasma species that inhabit the swine respiratory tract facilitated the identification of some characteristics that may be related to their different behaviors. M. hyopneumoniae and M. flocculare display many differences that may help to explain why one species is pathogenic and the other is considered to be commensal. However, it was not possible to identify specific virulence determinant factors that could explain the differences in the pathogenicity of the analyzed species. The M. hyorhinis genome contains differences in some components involved in metabolism and evasion of the host’s immune system that may contribute to its growth aggressiveness. Several horizontal gene transfer events were identified. The phylogenomic analysis places M. hyopneumoniae, M. flocculare and M. hyorhinis in the hyopneumoniae clade. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2015-02-14T02:19:12Z |
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Estrangeiro info:eu-repo/semantics/article |
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http://hdl.handle.net/10183/110154 |
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BMC Genomics. London. Vol. 14,(Mar. 2013), e175, 17 p. |
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openAccess |
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