Bovine ringworm : detection of Trichophyton verrucosum by SYBR-Green real-time PCR

Detalhes bibliográficos
Autor(a) principal: Spanamberg, Andréia
Data de Publicação: 2023
Outros Autores: Ravazzolo, Ana Paula, Araujo, Ricardo Pinto, Franceschi, Natália Tomazi, Ferreiro, Laerte
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/261876
Resumo: Trichophyton verrucosum is the most commonly dermatophyte involved in cattle ringworm. This work reported a case of bovine dermatophytosis due to Trichophyton verrucosum detected from the clinical sample by SYBR-Green real-time PCR. The strategy was based on the DNA extraction directly from the infected hair followed by realtime PCR and melting-point analysis. A faster and differential diagnosis was observed when compared to the conventional mycological methodology for detection and identification of Trichophyton verrucosum.
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spelling Spanamberg, AndréiaRavazzolo, Ana PaulaAraujo, Ricardo PintoFranceschi, Natália TomaziFerreiro, Laerte2023-07-08T03:35:50Z20232211-7539http://hdl.handle.net/10183/261876001169483Trichophyton verrucosum is the most commonly dermatophyte involved in cattle ringworm. This work reported a case of bovine dermatophytosis due to Trichophyton verrucosum detected from the clinical sample by SYBR-Green real-time PCR. The strategy was based on the DNA extraction directly from the infected hair followed by realtime PCR and melting-point analysis. A faster and differential diagnosis was observed when compared to the conventional mycological methodology for detection and identification of Trichophyton verrucosum.application/pdfengMedical mycology case reports. Amsterdam. Vol. 39 (Mar. 2023), p. 34-37Técnicas de diagnóstico molecularReação em cadeia da polimeraseDermatofitosesTrichophytonBovinosTrichophyton verrucosumDermatophytesRingwormHairCattleBovine ringworm : detection of Trichophyton verrucosum by SYBR-Green real-time PCREstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001169483.pdf.txt001169483.pdf.txtExtracted Texttext/plain18300http://www.lume.ufrgs.br/bitstream/10183/261876/2/001169483.pdf.txt23f3ce91ce38a09c9defb15c9c15aab0MD52ORIGINAL001169483.pdfTexto completo (inglês)application/pdf1401515http://www.lume.ufrgs.br/bitstream/10183/261876/1/001169483.pdf34b13abcb596fd413f4f7f504509db23MD5110183/2618762023-07-09 03:37:02.616062oai:www.lume.ufrgs.br:10183/261876Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-07-09T06:37:02Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Bovine ringworm : detection of Trichophyton verrucosum by SYBR-Green real-time PCR
title Bovine ringworm : detection of Trichophyton verrucosum by SYBR-Green real-time PCR
spellingShingle Bovine ringworm : detection of Trichophyton verrucosum by SYBR-Green real-time PCR
Spanamberg, Andréia
Técnicas de diagnóstico molecular
Reação em cadeia da polimerase
Dermatofitoses
Trichophyton
Bovinos
Trichophyton verrucosum
Dermatophytes
Ringworm
Hair
Cattle
title_short Bovine ringworm : detection of Trichophyton verrucosum by SYBR-Green real-time PCR
title_full Bovine ringworm : detection of Trichophyton verrucosum by SYBR-Green real-time PCR
title_fullStr Bovine ringworm : detection of Trichophyton verrucosum by SYBR-Green real-time PCR
title_full_unstemmed Bovine ringworm : detection of Trichophyton verrucosum by SYBR-Green real-time PCR
title_sort Bovine ringworm : detection of Trichophyton verrucosum by SYBR-Green real-time PCR
author Spanamberg, Andréia
author_facet Spanamberg, Andréia
Ravazzolo, Ana Paula
Araujo, Ricardo Pinto
Franceschi, Natália Tomazi
Ferreiro, Laerte
author_role author
author2 Ravazzolo, Ana Paula
Araujo, Ricardo Pinto
Franceschi, Natália Tomazi
Ferreiro, Laerte
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Spanamberg, Andréia
Ravazzolo, Ana Paula
Araujo, Ricardo Pinto
Franceschi, Natália Tomazi
Ferreiro, Laerte
dc.subject.por.fl_str_mv Técnicas de diagnóstico molecular
Reação em cadeia da polimerase
Dermatofitoses
Trichophyton
Bovinos
topic Técnicas de diagnóstico molecular
Reação em cadeia da polimerase
Dermatofitoses
Trichophyton
Bovinos
Trichophyton verrucosum
Dermatophytes
Ringworm
Hair
Cattle
dc.subject.eng.fl_str_mv Trichophyton verrucosum
Dermatophytes
Ringworm
Hair
Cattle
description Trichophyton verrucosum is the most commonly dermatophyte involved in cattle ringworm. This work reported a case of bovine dermatophytosis due to Trichophyton verrucosum detected from the clinical sample by SYBR-Green real-time PCR. The strategy was based on the DNA extraction directly from the infected hair followed by realtime PCR and melting-point analysis. A faster and differential diagnosis was observed when compared to the conventional mycological methodology for detection and identification of Trichophyton verrucosum.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-07-08T03:35:50Z
dc.date.issued.fl_str_mv 2023
dc.type.driver.fl_str_mv Estrangeiro
info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/261876
dc.identifier.issn.pt_BR.fl_str_mv 2211-7539
dc.identifier.nrb.pt_BR.fl_str_mv 001169483
identifier_str_mv 2211-7539
001169483
url http://hdl.handle.net/10183/261876
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Medical mycology case reports. Amsterdam. Vol. 39 (Mar. 2023), p. 34-37
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
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reponame_str Repositório Institucional da UFRGS
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