Coagulase-negative staphylococci in outpatient routines : the implications of switching from CLSI to BrCAST/EUCAST guidelines
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/217150 |
Resumo: | Coagulase-negative staphylococci (CoNS) are frequently isolated in clinical specimens and are important reservoirs of resistance genes. In 2019, the Brazilian government set the BrCAST/EUCAST (Brazilian Committee on Antimicrobial Susceptibility Testing) guidelines as the national standard, resulting in changes in the interpretation of CoNS susceptibility tests. From outpatients, disk-diffusion susceptibility of 65 CoNS cultures were evaluated and compared using classification criteria from both CLSI and BrCAST/EUCAST. The isolates were identified using matrix assisted laser desorption ionization–time of flight (MALDI-TOF), and the presence of the mecA gene was determined. The most prevalent species were Staphylococcus saprophyticus (32.3%), S. haemolyticus (18.5%), and S. epidermidis (9.2%). Almost perfect agreement was seen between the guidelines, except concerning oxacillin and gentamicin, and the prevalence of multidrug resistant isolates increased with the use of BrCAST/EUCAST. Of all, 15 (23.1%) isolates, mainly S. epidermidis and S. haemolyticus, were positive for the mecA gene, and only three were detected when using CLSI or BrCAST/EUCAST disk-diffusion screening. This, using either guideline, could reveal the difficulty of determining oxacillin resistance. Using warning zones or molecular methods might well be indicated for CoNS. In conclusion, adoption of the BrCAST/EUCAST guidelines will result in certain artificial changes in epidemiological susceptibility profiles, and clinicians and institutions should be aware of the possible implications. |
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Perez, Vinícius PietaCarvalho, Jéssica Karoliny Baptista PortoOliveira, Marianne Schrader deRossato, Adriana MedianeiraDani, CarolineCorção, GertrudesD'Azevedo, Pedro Alves2021-01-08T04:06:46Z20201517-8382http://hdl.handle.net/10183/217150001120416Coagulase-negative staphylococci (CoNS) are frequently isolated in clinical specimens and are important reservoirs of resistance genes. In 2019, the Brazilian government set the BrCAST/EUCAST (Brazilian Committee on Antimicrobial Susceptibility Testing) guidelines as the national standard, resulting in changes in the interpretation of CoNS susceptibility tests. From outpatients, disk-diffusion susceptibility of 65 CoNS cultures were evaluated and compared using classification criteria from both CLSI and BrCAST/EUCAST. The isolates were identified using matrix assisted laser desorption ionization–time of flight (MALDI-TOF), and the presence of the mecA gene was determined. The most prevalent species were Staphylococcus saprophyticus (32.3%), S. haemolyticus (18.5%), and S. epidermidis (9.2%). Almost perfect agreement was seen between the guidelines, except concerning oxacillin and gentamicin, and the prevalence of multidrug resistant isolates increased with the use of BrCAST/EUCAST. Of all, 15 (23.1%) isolates, mainly S. epidermidis and S. haemolyticus, were positive for the mecA gene, and only three were detected when using CLSI or BrCAST/EUCAST disk-diffusion screening. This, using either guideline, could reveal the difficulty of determining oxacillin resistance. Using warning zones or molecular methods might well be indicated for CoNS. In conclusion, adoption of the BrCAST/EUCAST guidelines will result in certain artificial changes in epidemiological susceptibility profiles, and clinicians and institutions should be aware of the possible implications.application/pdfengBrazilian journal of microbiology. Rio de Janeiro. Vol. 51, no. 3 (Sept. 2020), p. 1071-1078Infecções estafilocócicasGuias como assuntoResistência microbiana a medicamentosAntimicrobial susceptibility testingBrazilian Committee on Antimicrobial Susceptibility TestingEuropean Committee on Antimicrobial Susceptibility TestingClinical Laboratory Standards InstituteCoagulase-negative staphylococciOxacillin resistanceCoagulase-negative staphylococci in outpatient routines : the implications of switching from CLSI to BrCAST/EUCAST guidelinesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001120416.pdf.txt001120416.pdf.txtExtracted Texttext/plain36879http://www.lume.ufrgs.br/bitstream/10183/217150/2/001120416.pdf.txt77b7ee06adfbebd7ee7a5c3bd0b35561MD52ORIGINAL001120416.pdfTexto completo (inglês)application/pdf728718http://www.lume.ufrgs.br/bitstream/10183/217150/1/001120416.pdf4bb4722730f55b0fcb5a9f02c22404c7MD5110183/2171502021-03-09 04:42:42.017114oai:www.lume.ufrgs.br:10183/217150Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-03-09T07:42:42Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Coagulase-negative staphylococci in outpatient routines : the implications of switching from CLSI to BrCAST/EUCAST guidelines |
title |
Coagulase-negative staphylococci in outpatient routines : the implications of switching from CLSI to BrCAST/EUCAST guidelines |
spellingShingle |
Coagulase-negative staphylococci in outpatient routines : the implications of switching from CLSI to BrCAST/EUCAST guidelines Perez, Vinícius Pieta Infecções estafilocócicas Guias como assunto Resistência microbiana a medicamentos Antimicrobial susceptibility testing Brazilian Committee on Antimicrobial Susceptibility Testing European Committee on Antimicrobial Susceptibility Testing Clinical Laboratory Standards Institute Coagulase-negative staphylococci Oxacillin resistance |
title_short |
Coagulase-negative staphylococci in outpatient routines : the implications of switching from CLSI to BrCAST/EUCAST guidelines |
title_full |
Coagulase-negative staphylococci in outpatient routines : the implications of switching from CLSI to BrCAST/EUCAST guidelines |
title_fullStr |
Coagulase-negative staphylococci in outpatient routines : the implications of switching from CLSI to BrCAST/EUCAST guidelines |
title_full_unstemmed |
Coagulase-negative staphylococci in outpatient routines : the implications of switching from CLSI to BrCAST/EUCAST guidelines |
title_sort |
Coagulase-negative staphylococci in outpatient routines : the implications of switching from CLSI to BrCAST/EUCAST guidelines |
author |
Perez, Vinícius Pieta |
author_facet |
Perez, Vinícius Pieta Carvalho, Jéssica Karoliny Baptista Porto Oliveira, Marianne Schrader de Rossato, Adriana Medianeira Dani, Caroline Corção, Gertrudes D'Azevedo, Pedro Alves |
author_role |
author |
author2 |
Carvalho, Jéssica Karoliny Baptista Porto Oliveira, Marianne Schrader de Rossato, Adriana Medianeira Dani, Caroline Corção, Gertrudes D'Azevedo, Pedro Alves |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Perez, Vinícius Pieta Carvalho, Jéssica Karoliny Baptista Porto Oliveira, Marianne Schrader de Rossato, Adriana Medianeira Dani, Caroline Corção, Gertrudes D'Azevedo, Pedro Alves |
dc.subject.por.fl_str_mv |
Infecções estafilocócicas Guias como assunto Resistência microbiana a medicamentos |
topic |
Infecções estafilocócicas Guias como assunto Resistência microbiana a medicamentos Antimicrobial susceptibility testing Brazilian Committee on Antimicrobial Susceptibility Testing European Committee on Antimicrobial Susceptibility Testing Clinical Laboratory Standards Institute Coagulase-negative staphylococci Oxacillin resistance |
dc.subject.eng.fl_str_mv |
Antimicrobial susceptibility testing Brazilian Committee on Antimicrobial Susceptibility Testing European Committee on Antimicrobial Susceptibility Testing Clinical Laboratory Standards Institute Coagulase-negative staphylococci Oxacillin resistance |
description |
Coagulase-negative staphylococci (CoNS) are frequently isolated in clinical specimens and are important reservoirs of resistance genes. In 2019, the Brazilian government set the BrCAST/EUCAST (Brazilian Committee on Antimicrobial Susceptibility Testing) guidelines as the national standard, resulting in changes in the interpretation of CoNS susceptibility tests. From outpatients, disk-diffusion susceptibility of 65 CoNS cultures were evaluated and compared using classification criteria from both CLSI and BrCAST/EUCAST. The isolates were identified using matrix assisted laser desorption ionization–time of flight (MALDI-TOF), and the presence of the mecA gene was determined. The most prevalent species were Staphylococcus saprophyticus (32.3%), S. haemolyticus (18.5%), and S. epidermidis (9.2%). Almost perfect agreement was seen between the guidelines, except concerning oxacillin and gentamicin, and the prevalence of multidrug resistant isolates increased with the use of BrCAST/EUCAST. Of all, 15 (23.1%) isolates, mainly S. epidermidis and S. haemolyticus, were positive for the mecA gene, and only three were detected when using CLSI or BrCAST/EUCAST disk-diffusion screening. This, using either guideline, could reveal the difficulty of determining oxacillin resistance. Using warning zones or molecular methods might well be indicated for CoNS. In conclusion, adoption of the BrCAST/EUCAST guidelines will result in certain artificial changes in epidemiological susceptibility profiles, and clinicians and institutions should be aware of the possible implications. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2021-01-08T04:06:46Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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info:eu-repo/semantics/publishedVersion |
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publishedVersion |
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http://hdl.handle.net/10183/217150 |
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1517-8382 |
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001120416 |
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http://hdl.handle.net/10183/217150 |
dc.language.iso.fl_str_mv |
eng |
language |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of microbiology. Rio de Janeiro. Vol. 51, no. 3 (Sept. 2020), p. 1071-1078 |
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info:eu-repo/semantics/openAccess |
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