Evaluation of antibiotics as a methodological procedure to inhibit free-living and biofilm bacteria in marine zooplankton culture
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/164197 |
Resumo: | There is a problem with keeping culture medium completely or partially free from bacteria. The use of prokaryotic metabolic inhibitors, such as antibiotics, is suggested as an alternative solution, although such substances should not harm non-target organisms. Thus, the aim of this study was to assess the effectiveness of antibiotic treatments in inhibiting free-living and biofilm bacteria and their half-life in artificial marine environment using the copepod Acartia tonsa as bioindicador of non-harmful antibiotic combinations. Regarding to results, the application of 0.025 g L-1 penicillin G potassium + 0.08 g L-1 streptomycin sulphate + 0.04 g L-1 neomycin sulphate showed great potential for use in marine cultures and scientific experiments without lethal effects to non-target organisms. The effect of this combination starts within the first six hours of exposure and reduces up to 93 % the bacterial density, but the half-life is short, requiring replacement. No adverse changes in water quality were observed within 168 hours of exposure. As a conclusion, we can infer that this treatment was an effective procedure for zooplankton cultures and scientific experiments with the aim of measuring the role of free-living and biofilm in the marine community. |
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Agostini, Vanessa OchiMacedo, Alexandre JoséMuxagata, Erik2017-07-21T02:43:20Z20160001-3765http://hdl.handle.net/10183/164197001015451There is a problem with keeping culture medium completely or partially free from bacteria. The use of prokaryotic metabolic inhibitors, such as antibiotics, is suggested as an alternative solution, although such substances should not harm non-target organisms. Thus, the aim of this study was to assess the effectiveness of antibiotic treatments in inhibiting free-living and biofilm bacteria and their half-life in artificial marine environment using the copepod Acartia tonsa as bioindicador of non-harmful antibiotic combinations. Regarding to results, the application of 0.025 g L-1 penicillin G potassium + 0.08 g L-1 streptomycin sulphate + 0.04 g L-1 neomycin sulphate showed great potential for use in marine cultures and scientific experiments without lethal effects to non-target organisms. The effect of this combination starts within the first six hours of exposure and reduces up to 93 % the bacterial density, but the half-life is short, requiring replacement. No adverse changes in water quality were observed within 168 hours of exposure. As a conclusion, we can infer that this treatment was an effective procedure for zooplankton cultures and scientific experiments with the aim of measuring the role of free-living and biofilm in the marine community.application/pdfengAnais da Academia Brasileira de Ciências. Vol. 88, supl. 1 (May 2016), p. 733-746Farmacologia clínicaAntibióticosAcaria tonsaAntimicrobialsExperimentMarine cultureEvaluation of antibiotics as a methodological procedure to inhibit free-living and biofilm bacteria in marine zooplankton cultureinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001015451.pdf001015451.pdfTexto completo (inglês)application/pdf6224935http://www.lume.ufrgs.br/bitstream/10183/164197/1/001015451.pdf73bd6215bc4910ed89655006428c4bb4MD51TEXT001015451.pdf.txt001015451.pdf.txtExtracted Texttext/plain49483http://www.lume.ufrgs.br/bitstream/10183/164197/2/001015451.pdf.txt311d4515366477e5c8710ff577266a79MD52THUMBNAIL001015451.pdf.jpg001015451.pdf.jpgGenerated Thumbnailimage/jpeg1876http://www.lume.ufrgs.br/bitstream/10183/164197/3/001015451.pdf.jpg35069deccfd9ec72a71a7c2983cd0517MD5310183/1641972020-11-20 05:16:18.684865oai:www.lume.ufrgs.br:10183/164197Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2020-11-20T07:16:18Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Evaluation of antibiotics as a methodological procedure to inhibit free-living and biofilm bacteria in marine zooplankton culture |
title |
Evaluation of antibiotics as a methodological procedure to inhibit free-living and biofilm bacteria in marine zooplankton culture |
spellingShingle |
Evaluation of antibiotics as a methodological procedure to inhibit free-living and biofilm bacteria in marine zooplankton culture Agostini, Vanessa Ochi Farmacologia clínica Antibióticos Acaria tonsa Antimicrobials Experiment Marine culture |
title_short |
Evaluation of antibiotics as a methodological procedure to inhibit free-living and biofilm bacteria in marine zooplankton culture |
title_full |
Evaluation of antibiotics as a methodological procedure to inhibit free-living and biofilm bacteria in marine zooplankton culture |
title_fullStr |
Evaluation of antibiotics as a methodological procedure to inhibit free-living and biofilm bacteria in marine zooplankton culture |
title_full_unstemmed |
Evaluation of antibiotics as a methodological procedure to inhibit free-living and biofilm bacteria in marine zooplankton culture |
title_sort |
Evaluation of antibiotics as a methodological procedure to inhibit free-living and biofilm bacteria in marine zooplankton culture |
author |
Agostini, Vanessa Ochi |
author_facet |
Agostini, Vanessa Ochi Macedo, Alexandre José Muxagata, Erik |
author_role |
author |
author2 |
Macedo, Alexandre José Muxagata, Erik |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Agostini, Vanessa Ochi Macedo, Alexandre José Muxagata, Erik |
dc.subject.por.fl_str_mv |
Farmacologia clínica Antibióticos |
topic |
Farmacologia clínica Antibióticos Acaria tonsa Antimicrobials Experiment Marine culture |
dc.subject.eng.fl_str_mv |
Acaria tonsa Antimicrobials Experiment Marine culture |
description |
There is a problem with keeping culture medium completely or partially free from bacteria. The use of prokaryotic metabolic inhibitors, such as antibiotics, is suggested as an alternative solution, although such substances should not harm non-target organisms. Thus, the aim of this study was to assess the effectiveness of antibiotic treatments in inhibiting free-living and biofilm bacteria and their half-life in artificial marine environment using the copepod Acartia tonsa as bioindicador of non-harmful antibiotic combinations. Regarding to results, the application of 0.025 g L-1 penicillin G potassium + 0.08 g L-1 streptomycin sulphate + 0.04 g L-1 neomycin sulphate showed great potential for use in marine cultures and scientific experiments without lethal effects to non-target organisms. The effect of this combination starts within the first six hours of exposure and reduces up to 93 % the bacterial density, but the half-life is short, requiring replacement. No adverse changes in water quality were observed within 168 hours of exposure. As a conclusion, we can infer that this treatment was an effective procedure for zooplankton cultures and scientific experiments with the aim of measuring the role of free-living and biofilm in the marine community. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2017-07-21T02:43:20Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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info:eu-repo/semantics/publishedVersion |
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article |
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http://hdl.handle.net/10183/164197 |
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0001-3765 |
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http://hdl.handle.net/10183/164197 |
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eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Anais da Academia Brasileira de Ciências. Vol. 88, supl. 1 (May 2016), p. 733-746 |
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openAccess |
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